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Product Info Summary
| SKU: | A11481 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-CKLF3 CMTM3 Antibody
SKU/Catalog Number
A11481
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-CKLF3 CMTM3 Antibody (Catalog # A11481). Tested in WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CKLF3 CMTM3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A11481)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from N-terminal of human CKLF3.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A11481 is reactive to CMTM3 in Human
Reconstitution
Calculated molecular weight
19714 MW
Background of Cmtm3
This gene belongs to the chemokine-like factor gene superfamily, a novel family that is similar to the chemokine and the transmembrane 4 superfamilies of signaling molecules. This gene is one of several chemokine-like factor genes located in a cluster on chromosome 16. Alternatively spliced transcript variants containing different 5' UTRs, but encoding the same protein, have been identified.
Han W., Genomics 81:609-617(2003).
Liu Y., Submitted (OCT-2002) to the EMBL/GenBank/DDBJ databases.
Ota T., Nat. Genet. 36:40-45(2004).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A11481 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Validation Images & Assay Conditions
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Western blot analysis of extracts from Jurkat cells and COS cells, using CKLF3 antibody A11481.
Protein Target Info & Infographic
Gene/Protein Information For CMTM3 (Source: Uniprot.org, NCBI)
Gene Name
CMTM3
Full Name
CKLF-like MARVEL transmembrane domain-containing protein 3
Weight
19714 MW
Superfamily
chemokine-like factor family
Alternative Names
CKLF-like MARVEL transmembrane domain-containing protein 3;Chemokine-like factor superfamily member 3;CMTM3;CKLFSF3;
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CMTM3, check out the CMTM3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CMTM3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CKLF3 CMTM3 Antibody (A11481)
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No publications found for A11481
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Customer Reviews
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Customer Q&As
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7 Customer Q&As for Anti-CKLF3 CMTM3 Antibody
Question
Do you have a BSA free version of anti-CKLF3 antibody A11481 available?
Verified Customer
Verified customer
Asked: 2020-04-28
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-CKLF3 antibody A11481 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-04-28
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for lung placenta using anti-CKLF3 antibody A11481. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-10-28
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-10-28
Question
Would anti-CKLF3 antibody A11481 work on canine WB with right testis?
Verified Customer
Verified customer
Asked: 2019-07-05
Answer
Our lab technicians have not validated anti-CKLF3 antibody A11481 on canine. You can run a BLAST between canine and the immunogen sequence of anti-CKLF3 antibody A11481 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated canine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in canine right testis in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-07-05
Question
I have a question about product A11481, anti-CKLF3 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-05-31
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A11481 anti-CKLF3 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-05-31
Question
I see that the anti-CKLF3 antibody A11481 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2018-01-11
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2018-01-11
Question
I was wanting to use your anti-CKLF3 antibody for WB for human lung placenta on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human lung placenta identification?
P. Li
Verified customer
Asked: 2017-04-27
Answer
As indicated on the product datasheet, A11481 anti-CKLF3 antibody has been tested for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human lung placenta in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-04-27
Question
Will A11481 anti-CKLF3 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
B. Huang
Verified customer
Asked: 2014-10-28
Answer
As indicated on the product datasheet, A11481 anti-CKLF3 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2014-10-28
