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Product Info Summary
| SKU: | A04351-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-CITED1 Antibody Picoband®
SKU/Catalog Number
A04351-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-CITED1 Antibody Picoband® catalog # A04351-1. Tested in ELISA, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-CITED1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04351-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human CITED1 recombinant protein (Position: M1-A130).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A04351-1 is reactive to CITED1 in Human
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
23 kDa
Calculated molecular weight
19.896kDa
Background of CITED1
Cbp/p300-interacting transactivator 1 is a protein that in humans is encoded by the CITED1 gene. This gene encodes a member of the CREB-binding protein/p300-interacting transactivator with Asp/Glu-rich C-terminal domain (CITED) family of proteins. The encoded protein, also known as melanocyte-specific gene 1, may function as a transcriptional coactivator and may play a role in pigmentation of melanocytes. Alternatively spliced transcript variants have been described.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A04351-1 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: human 293T whole cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of CITED1 using anti-CITED1 antibody (A04351-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human 293T whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CITED1 antigen affinity purified polyclonal antibody (Catalog # A04351-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CITED1 at approximately 23 kDa. The expected band size for CITED1 is at 23 kDa.
Protein Target Info & Infographic
Gene/Protein Information For CITED1 (Source: Uniprot.org, NCBI)
Gene Name
CITED1
Full Name
Cbp/p300-interacting transactivator 1
Weight
19.896kDa
Superfamily
CITED family
Alternative Names
Vasoactive intestinal polypeptide receptor 1; VIP-R-1; Pituitary adenylate cyclase-activating polypeptide type II receptor; PACAP type II receptor; PACAP-R-2; PACAP-R2; VPAC1; VIPR1 CITED1 MSG1 Cbp/p300 interacting transactivator with Glu/Asp rich carboxy-terminal domain 1 cbp/p300-interacting transactivator 1|melanocyte-specific gene 1|melanocyte-specific protein 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CITED1, check out the CITED1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CITED1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CITED1 Antibody Picoband® (A04351-1)
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