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Product Info Summary
| SKU: | A01952-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-IGFBP5 Antibody Picoband®
SKU/Catalog Number
A01952-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-IGFBP5 Antibody Picoband® catalog # A01952-1. Tested in ELISA, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-IGFBP5 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01952-1)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human IGFBP5 recombinant protein (Position: A60-E272). Human IGFBP5 shares 96.7% and 96.2% amino acid (aa) sequence identity with mouse and rat IGFBP5, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A01952-1 is reactive to IGFBP5 in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
31 kDa
Calculated molecular weight
30570 MW
Background of IGFBP-5
Insulin-like growth factor-binding protein 5 is a protein that in humans is encoded by the IGFBP5 gene. The expression of IGFBP5 by stable transfection and adenovirus-mediated infection is inhibitory to growth in 2 human breast cancer cell lines. IGFBP5 expression leads to G2/M cell cycle arrest and apoptosis. Stable expression of IGFBP5 in the breast cancer cell lines also inhibits the formation and growth of tumors following injection in athymic mice. It is concluded that IGFBP5 is a growth inhibitor and proapoptotic agent in breast cancer cells. Additionally, IGFBP-5 is expressed by fibroblasts, myoblasts and osteoblasts, making it the predominant IGFBP found in bone extracts. It has a strong affinity for hydroxyapatite, allowing it to bind to bone cells. When bound to extracellular matrix, IGFBP-5 is protected from proteolysis and potentiates IGF activity, but when it is soluble, IGFBP-5 is cleaved to a biologically inactive 21 kDa fragment (1, 2).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01952-1 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
ELISA, 0.1-0.5μg/ml, -
Positive Control
WB: human PC-3 whole cell, human Hela whole cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of IGFBP5 using anti-IGFBP5 antibody (A01952-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human PC-3 whole cell lysates,
Lane 2: human Hela whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IGFBP5 antigen affinity purified polyclonal antibody (Catalog # A01952-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IGFBP5 at approximately 31KD. The expected band size for IGFBP5 is at 31KD.
Protein Target Info & Infographic
Gene/Protein Information For IGFBP5 (Source: Uniprot.org, NCBI)
Gene Name
IGFBP5
Full Name
Insulin-like growth factor-binding protein 5
Weight
30570 MW
Alternative Names
Insulin-like growth factor-binding protein 5;IBP-5;IGF-binding protein 5;IGFBP-5;IGFBP5;IBP5; IGFBP5 IBP5 insulin like growth factor binding protein 5 insulin-like growth factor-binding protein 5|IBP-5|IGF-binding protein 5|IGFBP-5
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on IGFBP5, check out the IGFBP5 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for IGFBP5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-IGFBP5 Antibody Picoband® (A01952-1)
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