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Product Info Summary
| SKU: | M04073 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Chromogranin B CHGB Monoclonal Antibody
View all Chromogranin B Antibodies
SKU/Catalog Number
M04073
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Chromogranin B CHGB Monoclonal Antibody catalog # M04073. Tested in WB application. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Chromogranin B CHGB Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M04073)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
ACFG-3
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Chromogranin B Secretogranin-1 is a neuroendocrine secretory granule protein, which may be the precursor for other biologically active peptides.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M04073 is reactive to CHGB in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
190 kDa
Calculated molecular weight
112035 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M04073 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
Positive Control
WB: HeLa cell, Raw 2647 cell
Validation Images & Assay Conditions
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Western blot analysis of Chromogranin B expression in (1) HeLa cell lysate; (2) Raw 264.7 cell lysate.
Protein Target Info & Infographic
Gene/Protein Information For CHGB (Source: Uniprot.org, NCBI)
Gene Name
CHGB
Full Name
Secretogranin-1
Weight
112035 MW
Superfamily
chromogranin/secretogranin protein family
Alternative Names
Serine/threonine-protein kinase N2;2.7.11.13;PKN gamma;Protein kinase C-like 2;Protein-kinase C-related kinase 2;PKN2;PRK2, PRKCL2; CHGB SCG1 chromogranin B secretogranin-1|cgB|secretogranin B|secretogranin I|sgI
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CHGB, check out the CHGB Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CHGB: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Chromogranin B CHGB Monoclonal Antibody (M04073)
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No publications found for M04073
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Customer Q&As
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4 Customer Q&As for Anti-Chromogranin B CHGB Monoclonal Antibody
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for liver using anti-Chromogranin B Monoclonal antibody M04073. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-10-16
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-10-16
Question
We are currently using anti-Chromogranin B Monoclonal antibody M04073 for rat tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on primate tissues as well?
Verified Customer
Verified customer
Asked: 2018-12-31
Answer
The anti-Chromogranin B Monoclonal antibody (M04073) has not been validated for cross reactivity specifically with primate tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-12-31
Question
I am looking for to test anti-Chromogranin B Monoclonal antibody M04073 on human liver for research purposes, then I may be interested in using anti-Chromogranin B Monoclonal antibody M04073 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
S. Miller
Verified customer
Asked: 2015-10-09
Answer
The products we sell, including anti-Chromogranin B Monoclonal antibody M04073, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2015-10-09
Question
I was wanting to use your anti-Chromogranin B Monoclonal antibody for WB for human liver on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human liver identification?
J. Gonzalez
Verified customer
Asked: 2014-12-16
Answer
As indicated on the product datasheet, M04073 anti-Chromogranin B Monoclonal antibody has been tested for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human liver in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2014-12-16
