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Product Info Summary
| SKU: | A08478 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-CHP2 Antibody
SKU/Catalog Number
A08478
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-CHP2 Antibody (Catalog # A08478). Tested in WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CHP2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A08478)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from internal of humanCHP2.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A08478 is reactive to CHP2 in Human, Mouse, Rat
Reconstitution
Calculated molecular weight
22452 MW
Background of CHP2
Functions as an integral cofactor in cell pH regulation by controlling plasma membrane-type Na+/H+ exchange activity. Binds to and activates SLC9A1/NHE1 in a serum-independent manner, thus increasing pH and protecting cells from serum deprivation-induced death. Also plays a role in the regulation of cell proliferation and tumor growth by increasing the phosphatase activity of PPP3CA in a calcium-dependent manner. Activator of the calcineurin/NFAT signaling pathway. Involved in the cytoplasmic translocation of the transcription factor NFATC3 to the nucleus.
Loftus B.J., Genomics 60:295-308(1999).
Wang Y., J. Immunol. 169:1102-1109(2002).
Pang T., J. Biol. Chem. 277:43771-43777(2002).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A08478 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Validation Images & Assay Conditions
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Western blot analysis of extracts from Jurkat cells, K562 cells and HT-29 cells, using CHP2 antibody A08478.
Protein Target Info & Infographic
Gene/Protein Information For CHP2 (Source: Uniprot.org, NCBI)
Gene Name
CHP2
Full Name
Calcineurin B homologous protein 2
Weight
22452 MW
Superfamily
calcineurin regulatory subunit family
Alternative Names
Calcineurin B homologous protein 2;Hepatocellular carcinoma-associated antigen 520;CHP2;HCA520; CHP2 calcineurin like EF-hand protein 2 calcineurin B homologous protein 2|hepatocellular carcinoma gene 520|hepatocellular carcinoma-associated 520
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CHP2, check out the CHP2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CHP2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CHP2 Antibody (A08478)
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No publications found for A08478
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Customer Reviews
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Customer Q&As
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3 Customer Q&As for Anti-CHP2 Antibody
Question
My question regarding product A08478, anti-CHP2 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-11-28
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A08478 anti-CHP2 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-11-28
Question
I see that the anti-CHP2 antibody A08478 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2017-07-25
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2017-07-25
Question
We are currently using anti-CHP2 antibody A08478 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on goat tissues as well?
D. Kulkarni
Verified customer
Asked: 2014-03-18
Answer
The anti-CHP2 antibody (A08478) has not been validated for cross reactivity specifically with goat tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2014-03-18
