Product Info Summary
SKU: | P03945 |
---|---|
Size: | 100 µl |
Reactive Species: | Human |
Host: | Rabbit |
Application: | WB |
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Product info
Product Name
Anti-Phospho-Che-1 (AATF) (Ser477) Antibody
SKU/Catalog Number
P03945
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-Phospho-Che-1 (AATF) (Ser477) Antibody (Catalog # P03945). Tested in WB applications. This antibody reacts with Human.
Storage & Handling
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. After date of receipt, stable for at least 1 year at -20°C.
Cite This Product
Anti-Phospho-Che-1 (AATF) (Ser477) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P03945)
Host
Rabbit
Contents
10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser477 of human Che-1, conjugated to keyhole limpet hemocyanin (KLH). Immunogen species is Human.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
P03945 is reactive to AATF in Human
Reconstitution
Observed Molecular Weight
52 kDa
Calculated molecular weight
63133 MW
Background of AATF
Che-1, also known as AATF (apoptosis-antagonizing transcription factor), is a RNA polymerase II-binding protein involved in regulating the transcription factor E2F and promoting cell cycle progression (Burgdorf et al., 2004). It has been suggested that Che-1 may act as a neuroprotective factor against Abeta-induced apoptosis by suppressing the production of reactive oxidative species (Xie et al., 2004). The checkpoint kinase Chk2 has been shown to phosphorylate Che-1 at Ser-477 contributing to the maintenance of the G2/M checkpoint induced by DNA damage (Bruno et al., 2006).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
P03945 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:1000
Validation Images & Assay Conditions
Click image to see more details
Western blot of HeLa cell lysate showing specific immunolabeling of the ~66 kDa Che-1 protein phosphorylated at Ser477 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is blocked by preadsorption of the phosphopeptide used as the antigen, but not by the corresponding non-phosphopeptide (not shown).
Protein Target Info & Infographic
Gene/Protein Information For AATF (Source: Uniprot.org, NCBI)
Gene Name
AATF
Full Name
Protein AATF
Weight
63133 MW
Superfamily
AATF family
Alternative Names
AATF antibody, AATF_HUMAN antibody, Apoptosis antagonizing transcription factor antibody, Apoptosis-antagonizing transcription factor antibody, BFR2 antibody, CHE 1 antibody, CHE1 antibody, DED antibody, Protein AATF antibody, Rb binding protein Che 1 antibody, Rb-binding protein Che-1 antibody AATF BFR2, CHE-1, CHE1, DED apoptosis antagonizing transcription factor protein AATF|rb-binding protein Che-1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on AATF, check out the AATF Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for AATF: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Phospho-Che-1 (AATF) (Ser477) Antibody (P03945)
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No publications found for P03945
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Customer Q&As
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1 Customer Q&As for Anti-Phospho-Che-1 (AATF) (Ser477) Antibody
Question
We are currently using anti-Phospho-Che-1 (AATF) (Ser477) antibody P03945 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on canine tissues as well?
Verified Customer
Verified customer
Asked: 2020-03-25
Answer
The anti-Phospho-Che-1 (AATF) (Ser477) antibody (P03945) has not been tested for cross reactivity specifically with canine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-03-25