Product Info Summary
SKU: | A03945 |
---|---|
Size: | 0.1 mg |
Reactive Species: | Human, Mouse |
Host: | Rabbit |
Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-Protein AATF AATF Antibody
SKU/Catalog Number
A03945
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Protein AATF AATF Antibody (Catalog # A03945). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse.
Storage & Handling
AATF antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-Protein AATF AATF Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03945)
Host
Rabbit
Contents
AATF Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
AATF antibody was raised against a 12 amino acid synthetic peptide from near the carboxy terminus of human AATF. The immunogen is located within the last 50 amino acids of AATF.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
At least four isoforms of RABEX5 are known to exist; this antibody will recognize all four. RABEX5 antibody is predicted to not cross-react with other LRRFIP family members.
Reactive Species
A03945 is reactive to AATF in Human, Mouse
Reconstitution
Observed Molecular Weight
68 kDa
Calculated molecular weight
63133 MW
Background of AATF
AATF (apoptosis antagonizing transcription factor) was initially discovered as an interaction partner of ZIP kinase (ZIPK), a member of death-associated protein (DAP) kinase family of pro-apoptotic serine/threonine kinases. AATF is a phosphoprotein containing an acidic region and a putative leucine zipper domain and nuclear localization signal, features which are typical of transcription factors. AATF inhibits the ZIPK-mediated pro-apoptotic pathway and may activate other anti-apoptotic pathways. Recently, it has also been shown to protect neural cells against oxidative damage induced by amyloid b-peptide and to inhibit aberrant production of the beta-peptide by interacting with Par-4 (prostate apoptosis response-4), another pro-apoptotic leucine zipper protein that is associated with neuronal degeneration in Alzheimer's disease (AD), suggesting that AATF may have potential therapeutic applications in both familial and sporadic forms of AD.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A03945 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
AATF antibody can be used for the detection of AATF by Western blot at 0.5 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of AATF in human small intestine cell lysate with AATF antibody at (A) 0.5
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Immunohistochemistry of AATF in human small intestine tissue with AATF antibody at 10 μg/mL.
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Immunofluorescence of AATF in Human Small Intestine cells with AATF antibody at 20 μg/mL.
Protein Target Info & Infographic
Gene/Protein Information For AATF (Source: Uniprot.org, NCBI)
Gene Name
AATF
Full Name
Protein AATF
Weight
63133 MW
Superfamily
AATF family
Alternative Names
DED, BFR2, CHE1, CHE-1, DED, HSPC277, Protein AATF, Apoptosis-antagonizing transcription factor AATF BFR2, CHE-1, CHE1, DED apoptosis antagonizing transcription factor protein AATF|rb-binding protein Che-1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on AATF, check out the AATF Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for AATF: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Protein AATF AATF Antibody (A03945)
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