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Product Info Summary
| SKU: | M00028 |
|---|---|
| Size: | 100ug/vial |
| Reactive Species: | Human, Mouse |
| Host: | Mouse |
| Application: | IF, IHC, WB |
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Product info
Product Name
Anti-CFTR (Cystic Fibrosis Transmembrane Conductance Regulator) Monoclonal Antibody
SKU/Catalog Number
M00028
Size
100ug/vial
Form
Liquid
Description
Boster Bio Anti-CFTR (Cystic Fibrosis Transmembrane Conductance Regulator) Monoclonal Antibody (Catalog # M00028). Tested in IF, WB, IHC applications. This antibody reacts with Human, Mouse.
Storage & Handling
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Cite This Product
Anti-CFTR (Cystic Fibrosis Transmembrane Conductance Regulator) Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00028)
Host
Mouse
Contents
Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Clonality
Monoclonal
Clone Number
Clone: SPM176
Isotype
IgG2a, kappa
Immunogen
Recombinant human CFTR fragment
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00028 is reactive to CFTR in Human, Mouse
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 100 µL
Calculated molecular weight
168142 MW
Background of CFTR
Recognizes a protein of 165-170kDa, identified as cystic fibrosis transmembrane conductance regulator (CFTR). CFTR is composed of two membrane-spanning domains (MSD), two nucleotide-binding domains (NBD), and an R domain. It is structurally similar to multidrug resistance (Mdr1) protein and both are members of the superfamily of ATP-binding cassette (ABC) transporters, also known as traffic ATPases, which are implicated in the movement of various substrates. The CFTR protein is a small conductance adenosine 3',5'-cyclic monophosphate (cAMP)-activated chloride ion channel found in the apical membranes of epithelia within the pancreas, airway, intestine, bile duct, sweat gland, and male genital ducts. CFTR is a valuable marker of human pancreatic duct cell development and differentiation.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00028 is guaranteed for IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunofluorescence (1-2ug/ml)
Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues is enhanced by heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0 for 45 min at 95°C followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.
Validation Images & Assay Conditions
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Formalin-fixed, paraffin-embedded Human Pancreas stained with Anti-CFTR Monoclonal Antibody (SPM176).
Protein Target Info & Infographic
Gene/Protein Information For CFTR (Source: Uniprot.org, NCBI)
Gene Name
CFTR
Full Name
Cystic fibrosis transmembrane conductance regulator
Weight
168142 MW
Superfamily
ABC transporter superfamily
Alternative Names
Cystic fibrosis transmembrane conductance regulator;CFTR;ATP-binding cassette sub-family C member 7;Channel conductance-controlling ATPase;3.6.3.49;cAMP-dependent chloride channel;CFTR;ABCC7; CFTR ABC35, ABCC7, CF/MRP, MRP7, TNR-CFTR, dJ760C5.1, CFTR CF transmembrane conductance regulator cystic fibrosis transmembrane conductance regulator|cAMP-dependent chloride channel|channel conductance-controlling ATPase|cystic fibrosis transmembrane conductance regulating|cystic fibrosis transmembrane conductance regulator (ATP-binding cassette sub-family C, member 7)
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CFTR, check out the CFTR Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CFTR: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CFTR (Cystic Fibrosis Transmembrane Conductance Regulator) Monoclonal Antibody (M00028)
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No publications found for M00028
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Customer Q&As
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5 Customer Q&As for Anti-CFTR (Cystic Fibrosis Transmembrane Conductance Regulator) Monoclonal Antibody
Question
Is this M00028 anti-CFTR (Cystic Fibrosis Transmembrane Conductance Regulator) Monoclonal antibody reactive to the isotypes of CFTR?
B. Baker
Verified customer
Asked: 2019-07-22
Answer
The immunogen of M00028 anti-CFTR (Cystic Fibrosis Transmembrane Conductance Regulator) Monoclonal antibody is Recombinant human full-length CFTR. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-07-22
Question
Is M00028 antibody raised against the extracellular part of CFTR protein?
Verified customer
Asked: 2019-07-10
Answer
The immunogen of the Anti-CFTR (Cystic Fibrosis Transmembrane Conductance Regulator) Monoclonal Antibody M00028 is last 200 amino acids at the end of the c-terminal and seems to be against the cytoplasmic portion of CFTR.
Boster Scientific Support
Answered: 2019-07-16
Question
Our lab want to know about to test anti-CFTR (Cystic Fibrosis Transmembrane Conductance Regulator) Monoclonal antibody M00028 on mouse body of pancreas for research purposes, then I may be interested in using anti-CFTR (Cystic Fibrosis Transmembrane Conductance Regulator) Monoclonal antibody M00028 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2018-11-22
Answer
The products we sell, including anti-CFTR (Cystic Fibrosis Transmembrane Conductance Regulator) Monoclonal antibody M00028, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-11-22
Question
We are currently using anti-CFTR (Cystic Fibrosis Transmembrane Conductance Regulator) Monoclonal antibody M00028 for mouse tissue, and we are happy with the IHC results. The species of reactivity given in the datasheet says human, mouse. Is it possible that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2018-10-23
Answer
The anti-CFTR (Cystic Fibrosis Transmembrane Conductance Regulator) Monoclonal antibody (M00028) has not been validated for cross reactivity specifically with bovine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-10-23
Question
I was wanting to use your anti-CFTR (Cystic Fibrosis Transmembrane Conductance Regulator) Monoclonal antibody for IHC for mouse body of pancreas on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse body of pancreas identification?
Verified Customer
Verified customer
Asked: 2018-08-13
Answer
You can see on the product datasheet, M00028 anti-CFTR (Cystic Fibrosis Transmembrane Conductance Regulator) Monoclonal antibody has been tested for IHC on human, mouse tissues. We have an innovator award program that if you test this antibody and show it works in mouse body of pancreas in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-08-13
