Anti-CFTR (Cystic Fibrosis Transmembrane Conductance Regulator) Monoclonal Antibody

Product Name

Anti-CFTR (Cystic Fibrosis Transmembrane Conductance Regulator) Monoclonal Antibody

View all CFTR Antibodies

SKU/Catalog Number

M00028

Size

100ug/vial

Form

Liquid

Description

Boster Bio Anti-CFTR (Cystic Fibrosis Transmembrane Conductance Regulator) Monoclonal Antibody (Catalog # M00028). Tested in IF, WB, IHC applications. This antibody reacts with Human, Mouse.

Storage & Handling

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Cite This Product

Anti-CFTR (Cystic Fibrosis Transmembrane Conductance Regulator) Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00028)

Host

Mouse

Contents

Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Clonality

Monoclonal

Clone Number

Clone: SPM176

Isotype

IgG2a, kappa

Immunogen

Recombinant human CFTR fragment

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M00028 is reactive to CFTR in Human, Mouse

Reconstitution

Restore with deionized water (or equivalent) for reconstitution volume of 100 µL

Calculated molecular weight

168142 MW

Background of CFTR

Recognizes a protein of 165-170kDa, identified as cystic fibrosis transmembrane conductance regulator (CFTR). CFTR is composed of two membrane-spanning domains (MSD), two nucleotide-binding domains (NBD), and an R domain. It is structurally similar to multidrug resistance (Mdr1) protein and both are members of the superfamily of ATP-binding cassette (ABC) transporters, also known as traffic ATPases, which are implicated in the movement of various substrates. The CFTR protein is a small conductance adenosine 3',5'-cyclic monophosphate (cAMP)-activated chloride ion channel found in the apical membranes of epithelia within the pancreas, airway, intestine, bile duct, sweat gland, and male genital ducts. CFTR is a valuable marker of human pancreatic duct cell development and differentiation.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

M00028 is guaranteed for IF, IHC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunofluorescence (1-2ug/ml)
Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues is enhanced by heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0 for 45 min at 95°C followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

Validation Images & Assay Conditions

Gene/Protein Information For CFTR (Source: Uniprot.org, NCBI)

Alternative Names

Cystic fibrosis transmembrane conductance regulator;CFTR;ATP-binding cassette sub-family C member 7;Channel conductance-controlling ATPase;3.6.3.49;cAMP-dependent chloride channel;CFTR;ABCC7; CFTR ABC35, ABCC7, CF/MRP, MRP7, TNR-CFTR, dJ760C5.1, CFTR CF transmembrane conductance regulator cystic fibrosis transmembrane conductance regulator|cAMP-dependent chloride channel|channel conductance-controlling ATPase|cystic fibrosis transmembrane conductance regulating|cystic fibrosis transmembrane conductance regulator (ATP-binding cassette sub-family C, member 7)

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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