Anti-COX4I2 Monoclonal Antibody

Product Name

Anti-COX4I2 Monoclonal Antibody

View all Cox4i2 Antibodies

SKU/Catalog Number

M10078

Size

100ul

Form

Liquid

Description

Boster Bio Anti-COX4I2 Monoclonal Antibody catalog # M10078. Tested in Flow Cytometry, IF, WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-COX4I2 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M10078)

Host

Mouse

Contents

Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.2% sodium azide, 50% glycerol.

Clonality

Monoclonal

Clone Number

2E8

Isotype

IgG

Immunogen

Purified recombinant human COX4I2 (C-terminal) protein fragments expressed in E.coli.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M10078 is reactive to Cox4i2 in Human

Applications

M10078 is guaranteed for Flow Cytometry, IF, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

20010 MW

Background of Cox4i2

Epithelial splicing regulatory protein 2 (Esrp-2) is an mRNA splicing factor that regulates the formation of epithelial cell-specific isoforms. It specifically regulates the expression of FGFR2-IIIb, an epithelial cell-specific isoform of FGFR2, and also regulates the splicing of CD44, CTNND1, ENAH, 3 transcripts that undergo changes in splicing during the epithelial-to-mesenchymal transition (EMT). Esrp-2 acts by directly binding specific sequences in mRNAs. It binds the GU-rich sequence motifs in the ISE/ISS-3, a cis-element regulatory region present in the mRNA of FGFR2.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:1000-1:2000
FC 1:100-1:200
IF 1:100-1:500

Validation Images & Assay Conditions

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Figure 3. Flow Cytometry validation of COX4I2 using Anti-COX4I2 Monoclonal Antibody (M10078).

Flow cytometric (FCM) analysis of K562 cells stained with COX4I2 Monoclonal Antibody (red)

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Figure 2. Immunofluorescent staining data of COX4I2 using Anti-COX4I2 Monoclonal Antibody (M10078).

Immunofluorescence (IF) analysis of HeLa cells using COX4I2 Monoclonal Antibody.

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Figure 1. Western blotting validation for Anti-COX4I2 Monoclonal Antibody M10078

Western Blot (WB) analysis using COX4I2 Monoclonal Antibody against mouse skeletal muscel

Gene/Protein Information For Cox4i2 (Source: Uniprot.org, NCBI)

Alternative Names

COX IV-2; COX4; COX4-2; COX4B; COX4-I2; COX4L2; COXIV-2; cytochrome c oxidase subunit 4 isoform 2, mitochondrial; cytochrome c oxidase subunit IV isoform 2 (lung); Cytochrome c oxidase subunit IV isoform 2; cytochrome c oxidase subunit IV-like 2; dJ857M17.2 Cox4i2|Cox4, Cox4b, CoxIV-2|cytochrome c oxidase subunit 4I2|cytochrome c oxidase subunit 4 isoform 2, mitochondrial|cytochrome c oxidase, subunit IVb

For more info on Cox4i2, check out the Cox4i2 Infographic

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In this infographic, you will see the following information for Cox4i2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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