Product Info Summary
SKU: | A30541 |
---|---|
Size: | 100ul |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | ELISA, IHC-P, WB, IF (paraffin) |
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Product info
Product Name
Anti-CELF-1 Antibody
View all CUGBP1/CELF1 Antibodies
SKU/Catalog Number
A30541
Size
100ul
Form
Liquid
Description
Boster Bio Anti-CELF-1 Antibody (Catalog# A30541). Tested in WB, IHC-P, IF (paraffin), ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CELF-1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A30541)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human CELF-1. Immunogen sequence location: 71-120
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A30541 is reactive to CELF1 in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
72 kDa
Calculated molecular weight
52063 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A30541 is guaranteed for ELISA, IHC-P, WB, IF (paraffin) Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western Blot, 1:500 - 1:2000
Immunohistochemistry, 1:100 - 1:300
ELISA, 1:40000
Not yet tested in other applications.
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of lysates from HeLa and HepG2 cells, using CELF-1 Antibody. The lane on the right is blocked with the synthesized peptide.
Protein Target Info & Infographic
Gene/Protein Information For CELF1 (Source: Uniprot.org, NCBI)
Gene Name
CELF1
Full Name
CUGBP Elav-like family member 1
Weight
52063 MW
Superfamily
CELF/BRUNOL family
Alternative Names
CELF1;BRUNOL2;CUGBP;CUGBP1;NAB50;CUGBP Elav-like family member 1;CELF-1;50 kDa nuclear polyadenylated RNA-binding protein;Bruno-like protein 2;CUG triplet repeat RNA-binding protein 1;CUG-BP1;CUG-BP- and ETR-3-like factor 1;Dead CELF1 BRUNOL2, CUG-BP, CUGBP, CUGBP1, EDEN-BP, NAB50, NAPOR, hNab50 CUGBP Elav-like family member 1 CUGBP Elav-like family member 1|50 kDa nuclear polyadenylated RNA-binding protein|CUG RNA-binding protein|CUG triplet repeat RNA-binding protein 1|CUG-BP- and ETR-3-like factor 1|EDEN-BP homolog|RNA-binding protein BRUNOL-2|bruno-like 2|bruno-like protein 2|deadenylation factor CUG-BP|embryo deadenylation element binding protein|embryo deadenylation element-binding protein homolog|nuclear polyadenylated RNA-binding protein, 50-kD
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CELF1, check out the CELF1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CELF1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CELF-1 Antibody (A30541)
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No publications found for A30541
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Customer Q&As
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5 Customer Q&As for Anti-CELF-1 Antibody
Question
We are currently using anti-CELF-1 antibody A30541 for mouse tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on primate tissues as well?
Verified Customer
Verified customer
Asked: 2019-12-20
Answer
The anti-CELF-1 antibody (A30541) has not been tested for cross reactivity specifically with primate tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-12-20
Question
Is a blocking peptide available for product anti-CELF-1 antibody (A30541)?
Verified Customer
Verified customer
Asked: 2019-12-03
Answer
We do provide the blocking peptide for product anti-CELF-1 antibody (A30541). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-12-03
Question
I have a question about product A30541, anti-CELF-1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-10-14
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A30541 anti-CELF-1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-10-14
Question
I was wanting to use to test anti-CELF-1 antibody A30541 on mouse brain for research purposes, then I may be interested in using anti-CELF-1 antibody A30541 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-03-26
Answer
The products we sell, including anti-CELF-1 antibody A30541, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-03-26
Question
Does anti-CELF-1 antibody A30541 work for WB with brain?
S. Taylor
Verified customer
Asked: 2015-08-28
Answer
According to the expression profile of brain, CELF1 is highly expressed in brain. So, it is likely that anti-CELF-1 antibody A30541 will work for WB with brain.
Boster Scientific Support
Answered: 2015-08-28