Anti-CDR1 Antibody Picoband™

Product Name

Anti-CDR1 Antibody Picoband™

View all CDR1 Antibodies

SKU/Catalog Number

A08472-1

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-CDR1 Antibody Picoband™ catalog # A08472-1. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-CDR1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A08472-1)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human CDR1 recombinant protein (Position: M1-R152).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A08472-1 is reactive to CDR1 in Human, Mouse

Applications

A08472-1 is guaranteed for ELISA, WB Boster Guarantee

Observed Molecular Weight

31 kDa

Calculated molecular weight

Background of CDR1

Cerebellar degeneration-related protein 1 is a protein that in humans is encoded by the CDR1 gene. Autoantibodies directed against the protein encoded by this intronless gene have been found in some patients with paraneoplastic cerebellar degeneration. The encoded protein contains several hexapeptide repeats.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 μg/ml, Human, Mouse
Direct ELISA, 0.1-0.5 μg/ml, Human

Validation Images & Assay Conditions

Click image to see more details

Figure 1. Western blot analysis of CDR1 using anti-CDR1 antibody (A08472-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human U-87MG whole cell lysates,
Lane 2: mouse RAW264.7 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CDR1 antigen affinity purified polyclonal antibody (Catalog # A08472-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CDR1 at approximately 31 kDa. The expected band size for CDR1 is at 31 kDa.

Gene/Protein Information For CDR1 (Source: Uniprot.org, NCBI)

Alternative Names

Cerebellar degeneration-related antigen 1 CDR1 CDR, CDR34, CDR62A cerebellar degeneration related protein 1 cerebellar degeneration-related antigen 1|cerebellar degeneration-related protein 1, 34kDa

For more info on CDR1, check out the CDR1 Infographic

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In this infographic, you will see the following information for CDR1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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