Anti-CDNF Antibody Picoband™

Product Name

Anti-CDNF Antibody Picoband™

View all Cdnf Antibodies

SKU/Catalog Number

A10069-2

Size

100 µg/vial

Form

Lyophilized

Description

Boster Bio Anti-CDNF Antibody Picoband™ catalog # A10069-2. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Mouse, Rat.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-CDNF Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A10069-2)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived mouse Cdnf recombinant protein (Position: Q25-L187).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

A10069-2 is reactive to Cdnf in Mouse, Rat

Applications

A10069-2 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee

Observed Molecular Weight

19-20 kDa

Calculated molecular weight

41409 MW

Background of Cdnf

Cerebral dopamine neurotrophic factor also known as ARMET-like protein 1 is a protein that in humans is encoded by the CDNF gene. Predicted to enable growth factor activity. Predicted to be involved in dopaminergic neuron differentiation and neuron projection development. Predicted to be located in extracellular region. Predicted to be active in endoplasmic reticulum and extracellular space. Is expressed in several structures, including brain; genitourinary system; hemolymphoid system gland; salivary gland; and skeletal muscle tissue. Orthologous to human CDNF (cerebral dopamine neurotrophic factor).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconstitution

Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 µg/ml, Mouse, Rat
Flow Cytometry (Fixed), 1-3 µg/1x10⁶ cells, , Mouse
Direct ELISA, 0.1-0.5 µg/ml, , Mouse

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of Cdnf using anti-Cdnf antibody (A10069-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat heart tissue lysates,
Lane 2: mouse heart tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cdnf antigen affinity purified polyclonal antibody (Catalog # A10069-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cdnf at approximately 19-20 kDa. The expected band size for Cdnf is at 21 kDa.

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Figure 2. Flow Cytometry analysis of HEPA1-6 cells using anti-Cdnf antibody (A10069-2).
Overlay histogram showing HEPA1-6 cells stained with A10069-2 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-Cdnf Antibody (A10069-2, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

Gene/Protein Information For Cdnf (Source: Uniprot.org, NCBI)

Alternative Names

arginine-rich, mutated in early stage tumors-like 1; ARMETL1; ARMET-like protein 1; CDNF; cerebral dopamine neurotrophic factor; Conserved dopamine neurotrophic factor Cdnf|9330140G23, Arme, Armetl1|cerebral dopamine neurotrophic factor|cerebral dopamine neurotrophic factor|ARMET-like protein 1|arginine-rich, mutated in early stage tumors-like 1|conserved dopamine neurotrophic factor

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