Click image to see more details
Product Info Summary
| SKU: | A10069-2 |
|---|---|
| Size: | 100 µg/vial |
| Reactive Species: | Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-CDNF Antibody Picoband®
SKU/Catalog Number
A10069-2
Size
100 µg/vial
Form
Lyophilized
Description
Boster Bio Anti-CDNF Antibody Picoband® catalog # A10069-2. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-CDNF Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A10069-2)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived mouse Cdnf recombinant protein (Position: Q25-L187).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
A10069-2 is reactive to CDNF in Mouse, Rat
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Observed Molecular Weight
19-20 kDa
Calculated molecular weight
41409 MW
Background of Cdnf
Cerebral dopamine neurotrophic factor also known as ARMET-like protein 1 is a protein that in humans is encoded by the CDNF gene. Predicted to enable growth factor activity. Predicted to be involved in dopaminergic neuron differentiation and neuron projection development. Predicted to be located in extracellular region. Predicted to be active in endoplasmic reticulum and extracellular space. Is expressed in several structures, including brain; genitourinary system; hemolymphoid system gland; salivary gland; and skeletal muscle tissue. Orthologous to human CDNF (cerebral dopamine neurotrophic factor).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A10069-2 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, , Mouse
ELISA, 0.1-0.5 μg/ml, , Mouse
Positive Control
WB: rat heart tissue, mouse heart tissue
FCM: HEPA1-6 cell
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of Cdnf using anti-Cdnf antibody (A10069-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat heart tissue lysates,
Lane 2: mouse heart tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cdnf antigen affinity purified polyclonal antibody (Catalog # A10069-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cdnf at approximately 19-20 kDa. The expected band size for Cdnf is at 21 kDa.
Click image to see more details
Figure 2. Flow Cytometry analysis of HEPA1-6 cells using anti-Cdnf antibody (A10069-2).
Overlay histogram showing HEPA1-6 cells stained with A10069-2 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-Cdnf Antibody (A10069-2, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For CDNF (Source: Uniprot.org, NCBI)
Gene Name
CDNF
Full Name
Cerebral dopamine neurotrophic factor
Weight
41409 MW
Superfamily
ARMET family
Alternative Names
Protein NDRG3;N-myc downstream-regulated gene 3 protein;NDRG3; Cdnf|9330140G23, Arme, Armetl1|cerebral dopamine neurotrophic factor|cerebral dopamine neurotrophic factor|ARMET-like protein 1|arginine-rich, mutated in early stage tumors-like 1|conserved dopamine neurotrophic factor
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CDNF, check out the CDNF Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CDNF: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CDNF Antibody Picoband® (A10069-2)
Hello CJ!
No publications found for A10069-2
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-CDNF Antibody Picoband®?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Anti-CDNF Antibody Picoband®
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
