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Product Info Summary
| SKU: | A04972 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-Protein NDRG4 NDRG4 Antibody
SKU/Catalog Number
A04972
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-Protein NDRG4 NDRG4 Antibody (Catalog # A04972). Tested in WB, IHC applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Protein NDRG4 NDRG4 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04972)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from internal of human NDRG4.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A04972 is reactive to NDRG4 in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
111 kDa
Calculated molecular weight
38459 MW
Background of Ndrg4
Contributes to the maintenance of intracerebral BDNF levels within the normal range, which is necessary for the preservation of spatial learning and the resistance to neuronal cell death caused by ischemic stress By similarity. May enhance growth factor-induced ERK1 and ERK2 phosphorylation, including that induced by PDGF and FGF. May attenuate NGF-promoted ELK1 phosphorylation in a microtubule-dependent manner.
Zhou R.-H., Genomics 73:86-97(2001).
Nishimoto S., Submitted (DEC-1998) to the EMBL/GenBank/DDBJ databases.
Qu X., Mol. Cell. Biochem. 229:35-44(2002).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A04972 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Immunohistochemistry: 1:50~1:100
Validation Images & Assay Conditions
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Western blot analysis of extracts from HepG2 cells and HT-29 cells, using NDRG4 antibody A04972.
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Immunohistochemistry analysis of paraffin-embedded human heart tissue using NDRG4 antibody A04972.
Protein Target Info & Infographic
Gene/Protein Information For NDRG4 (Source: Uniprot.org, NCBI)
Gene Name
NDRG4
Full Name
Protein NDRG4
Weight
38459 MW
Superfamily
NDRG family
Alternative Names
Protein NDRG4;Brain development-related molecule 1;N-myc downstream-regulated gene 4 protein;Vascular smooth muscle cell-associated protein 8;SMAP-8;NDRG4;BDM1, KIAA1180; Ndrg4|Bdm1, Ndr4, smap8|NDRG family member 4|protein NDRG4|N-myc downstream regulated 4|N-myc downstream regulated gene 4|brain development-related molecule 1|development-related protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on NDRG4, check out the NDRG4 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for NDRG4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Protein NDRG4 NDRG4 Antibody (A04972)
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No publications found for A04972
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Customer Reviews
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Customer Q&As
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13 Customer Q&As for Anti-Protein NDRG4 NDRG4 Antibody
Question
Does A04972 anti-NDRG4 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-03-02
Answer
As indicated on the product datasheet, A04972 anti-NDRG4 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-03-02
Question
We are currently using anti-NDRG4 antibody A04972 for mouse tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on primate tissues as well?
Verified Customer
Verified customer
Asked: 2020-01-24
Answer
The anti-NDRG4 antibody (A04972) has not been validated for cross reactivity specifically with primate tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-01-24
Question
Is a blocking peptide available for product anti-NDRG4 antibody (A04972)?
Verified Customer
Verified customer
Asked: 2019-12-24
Answer
We do provide the blocking peptide for product anti-NDRG4 antibody (A04972). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-12-24
Question
I was wanting to use your anti-NDRG4 antibody for WB for mouse hippocampus thalamus on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse hippocampus thalamus identification?
V. Mitchell
Verified customer
Asked: 2019-10-23
Answer
It shows on the product datasheet, A04972 anti-NDRG4 antibody has been validated for IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse hippocampus thalamus in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-10-23
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for hippocampus thalamus using anti-NDRG4 antibody A04972. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-10-03
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-10-03
Question
Is this A04972 anti-NDRG4 antibody reactive to the isotypes of NDRG4?
Verified Customer
Verified customer
Asked: 2018-06-15
Answer
The immunogen of A04972 anti-NDRG4 antibody is Synthesized peptide derived from internal of human NDRG4. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-06-15
Question
Would anti-NDRG4 antibody A04972 work for WB with hippocampus thalamus?
C. Williams
Verified customer
Asked: 2018-04-16
Answer
According to the expression profile of hippocampus thalamus, NDRG4 is highly expressed in hippocampus thalamus. So, it is likely that anti-NDRG4 antibody A04972 will work for WB with hippocampus thalamus.
Boster Scientific Support
Answered: 2018-04-16
Question
Would anti-NDRG4 antibody A04972 work on monkey IHC with hippocampus thalamus?
Verified Customer
Verified customer
Asked: 2018-02-01
Answer
Our lab technicians have not validated anti-NDRG4 antibody A04972 on monkey. You can run a BLAST between monkey and the immunogen sequence of anti-NDRG4 antibody A04972 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated monkey samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in monkey hippocampus thalamus in IHC, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-02-01
Question
Is there a BSA free version of anti-NDRG4 antibody A04972 available?
Verified Customer
Verified customer
Asked: 2018-01-05
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-NDRG4 antibody A04972 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-01-05
Question
See below the WB image, lot number and protocol we used for hippocampus thalamus using anti-NDRG4 antibody A04972. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2017-11-27
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-11-27
Question
I have a question about product A04972, anti-NDRG4 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2017-09-06
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A04972 anti-NDRG4 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-09-06
Question
you antibody to test anti-NDRG4 antibody A04972 on mouse hippocampus thalamus for research purposes, then I may be interested in using anti-NDRG4 antibody A04972 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
N. Wu
Verified customer
Asked: 2015-08-06
Answer
The products we sell, including anti-NDRG4 antibody A04972, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2015-08-06
Question
I see that the anti-NDRG4 antibody A04972 works with WB, what is the protocol used to produce the result images on the product page?
J. Evans
Verified customer
Asked: 2013-10-18
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2013-10-18
