Anti-ZBTB7B Antibody (C-term)

Product Name

Anti-ZBTB7B Antibody (C-term)

View all Th-POK Antibodies

SKU/Catalog Number

A06831

Size

80 µl

Description

Boster Bio Anti-ZBTB7B Antibody (C-term) (Catalog # A06831). Tested in IF, WB application(s). This antibody reacts with Human.

Storage & Handling

Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Cite This Product

Anti-ZBTB7B Antibody (C-term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06831)

Host

Rabbit

Contents

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

This ZBTB7B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 440-469 amino acids from the C-terminal region of human ZBTB7B.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A06831 is reactive to Zbtb7b in Human

Applications

A06831 is guaranteed for IF, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

58027 Da

Background of Th-POK

ZBTB7B is a transcription regulator that acts as a key regulator of lineage commitment of immature T-cell precursors. It is necessary and sufficient for commitment of CD4 lineage, while its absence causes CD8 commitment. Development of immature T-cell precursors (thymocytes) to either the CD4 helper or CD8 killer T-cell lineages correlates precisely with their T-cell receptor specificity for major histocompatibility complex class II or class I molecules, respectively. ZBTB7B is a transcriptional repressor of the collagen COL1A1 and COL1A2 genes. It may also function as a repressor of fibronectin and possibly other extracellular matrix genes.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

IF: 1:10-1:50
WB: 1:1000

Validation Images & Assay Conditions

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Fluorescent confocal image of Hela cell stained with ZBTB7B Antibody (C-term). Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0. 1%, 10 min), then incubated with ZBTB7B primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃). Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin at 37℃). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). ZBTB7B immunoreactivity is localized to Nucleus significantly.

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All lanes : Anti-ZBTB7B Antibody (C-term) at 1:1000 dilution
Lane 1: Hela whole cell lysate
Lane 2: HepG2 whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 58 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.

Gene/Protein Information For Zbtb7b (Source: Uniprot.org, NCBI)

Alternative Names

c-Krox; hcKroxc-Krox; Krueppel-related zinc finger protein cKrox; kruppel-related zinc finger protein hcKrox; T-helper-inducing POZ/Krueppel-like factor; ThPOK; Th-POK; ZBTB15; ZBTB15zinc finger protein 67 homolog (mouse); ZBTB7B; ZFP67; zfp-67; zinc finger and BTB domain containing 15; zinc finger and BTB domain containing 7B; Zinc finger and BTB domain-containing protein 15; zinc finger and BTB domain-containing protein 7B; Zinc finger protein 67 homolog; Zinc finger protein 857B; Zinc finger protein Th-POK; ZNF857B; ZNF857BDKFZp686G01254 Zbtb7b|Thp, Thpok, Zfp6, Zfp67, c-Kr, c-Krox|zinc finger and BTB domain containing 7B|zinc finger and BTB domain-containing protein 7B|T-helper-inducing POZ/Krueppel-like factor|Vzinc finger and BTB domain containing 7B|krueppel-related zinc finger protein cKrox|zinc finger protein 67|zinc finger protein Th-POK

For more info on Zbtb7b, check out the Zbtb7b Infographic

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In this infographic, you will see the following information for Zbtb7b: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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