Product Info Summary
SKU: | M01054 |
---|---|
Size: | 100 μl |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | Flow Cytometry, IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-YB1 YBX1 Rabbit Monoclonal Antibody
SKU/Catalog Number
M01054
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-YB1 YBX1 Rabbit Monoclonal Antibody catalog # M01054. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-YB1 YBX1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01054)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
DDB-25
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human YB1
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M01054 is reactive to YBX1 in Human, Mouse, Rat
Applications
M01054 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
45 kDa
Calculated molecular weight
35924 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:50
FC 1:50
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of YB1 using anti-YB1 antibody (M01054).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human MCF-7 whole cell lysates,
Lane 3: human Jurkat whole cell lysates,
Lane 4: human T-47D whole cell lysates,
Lane 5: rat spleen tissue lysates,
Lane 6: rat PC-12 whole cell lysates,
Lane 7: mouse spleen tissue lysates,
Lane 8: mouse RAW264.7 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-YB1 antigen affinity purified monoclonal antibody (Catalog # M01054) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:1000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for YB1 at approximately 48 kDa. The expected band size for YB1 is at 36 kDa.
Click image to see more details
Immunohistochemical analysis of paraffin-embedded human kidney, using YB1 Antibody.
Click image to see more details
Immunofluorescent analysis of MCF-7 cells, using YB1 Antibody.
Protein Target Info & Infographic
Gene/Protein Information For YBX1 (Source: Uniprot.org, NCBI)
Gene Name
YBX1
Full Name
Y-box-binding protein 1
Weight
35924 MW
Superfamily
YBX1 family
Alternative Names
BP-8; CBF-A; class II, Y box-binding protein I; CSDA2; CSDB; DBPB CCAAT-binding transcription factor I subunit A; EFI-A; Enhancer factor I subunit A; MDR-NF1; MGC104858; MGC110976; MGC117250; NSEP1 Y-box-binding protein 1; nuclease-sensitive element-binding protein 1; Y box binding protein 1; YB1 DNA-binding protein B; YB-1 Y-box transcription factor; YBX1 YBX1 BP-8, CBF-A, CSDA2, CSDB, DBPB, EFI-A, MDR-NF1, NSEP-1, NSEP1, YB-1, YB1 Y-box binding protein 1 Y-box-binding protein 1|CCAAT-binding transcription factor I subunit A|DNA-binding protein B|Y-box transcription factor|enhancer factor I subunit A|major histocompatibility complex, class II, Y box-binding protein I|nuclease-sensitive element-binding protein 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on YBX1, check out the YBX1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for YBX1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-YB1 YBX1 Rabbit Monoclonal Antibody (M01054)
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