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Product Info Summary
| SKU: | A00166 |
|---|---|
| Size: | 100uL |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-Cyclin-dependent kinase 2 cdk2 Antibody
SKU/Catalog Number
A00166
Size
100uL
Form
Liquid (sterile filtered)
Description
Boster Bio Anti-Cyclin-dependent kinase 2 cdk2 Antibody (Catalog # A00166). Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. (Ship on dry ice.)
Cite This Product
Anti-Cyclin-dependent kinase 2 cdk2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00166)
Host
Rabbit
Contents
0.01% (w/v) Sodium Azide
Clonality
Polyclonal
Isotype
Antiserum
Immunogen
Anti-cdk2 antibody was prepared from whole rabbit serum by repeated immunizations with a synthetic peptide corresponding to the C-terminus of the human cdk-2 protein conjugated to Keyhole Limpet Hemocyanin (KLH).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A00166 is reactive to CDK2 in Human, Mouse, Rat
Reconstitution
Calculated molecular weight
33930 MW
Background of CDK2
Anti cdk2 antibody recognizes a protein encoded by a gene member of the Ser/Thr protein kinase family. This protein kinase is highly similar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2. It is a catalytic subunit of the cyclin-dependent protein kinase complex, whose activity is restricted to the G1-S phase, and essential for cell cycle G1/S phase transition. This protein associates with and regulated by the regulatory subunits of the complex including cyclin A or E, CDK inhibitor p21Cip1 (CDKN1A) and p27Kip1 (CDKN1B). Its activity is also regulated by its protein phosphorylation. Two alternatively spliced variants and multiple transcription initiation sites of this gene have been reported.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00166 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA: 1:1,000 - 1:5,000
IHC: 1:200 - 1:1,000
IF Microscopy: User optimized
IP: 1:100
WB: 1:200 - 1:1,000
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of cdk2 expression in HELA whole cell lysates (run in duplicate). cdk2 at 32-33KD was detected using rabbit anti-cdk2 Antigen Affinity purified polyclonal antibody (Catalog # A00166) at 1:200. The blot was developed using chemiluminescence (ECL) method (Catalog # EK1002).
Click image to see more details
cdk2 was detected in paraffin-embedded sections of human skin tissues using rabbit anti-cdk2 Antigen Affinity purified polyclonal antibody (Catalog # A00166) at 1:500. The immunohistochemical section was developed using SABC method (Catalog # SA1022).
Protein Target Info & Infographic
Gene/Protein Information For CDK2 (Source: Uniprot.org, NCBI)
Gene Name
CDK2
Full Name
Cyclin-dependent kinase 2
Weight
33930 MW
Superfamily
protein kinase superfamily
Alternative Names
Cdk2, Cdc2 related protein kinase, Cdk 2, CDKN2, Cell division kinase 2, Cell division protein kinase 2, Cyclin dependent kinase 2, p33 protein kinase, cdk CDK2 CDKN2, p33(CDK2) cyclin dependent kinase 2 cyclin-dependent kinase 2|cdc2-related protein kinase|cell division protein kinase 2|p33 protein kinase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CDK2, check out the CDK2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CDK2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Cyclin-dependent kinase 2 cdk2 Antibody (A00166)
Hello CJ!
A00166 has been cited in 5 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
IL-2R%u03B1 up-regulation is mediated by latent membrane protein 1 and promotes lymphomagenesis and chemotherapy resistance in natural killer/T-cell %u2026
Platycodin D, a metabolite of Platycodin grandiflorum, inhibits highly metastatic MDA-MB-231 breast cancer growth in vitro and in vivo by targeting the MDM2 %u2026
Enterovirus 71 mediates cell cycle arrest in S phase through non-structural protein 3D
Rheum emodin inhibits enterovirus 71 viral replication and affects the host cell cycle environment
TALENs-directed?knockout?of the?full-length?transcription?factor?Nrf1? that?represses?malignantbehaviour?of?human?hepatocellular?carcinoma?(HepG2)?cells
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Customer Reviews
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Customer Q&As
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15 Customer Q&As for Anti-Cyclin-dependent kinase 2 cdk2 Antibody
Question
Here is the WB image, lot number and protocol we used for leukemic t-cell using anti-cdk2 antibody A00166. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-03-06
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-03-06
Question
Does anti-cdk2 antibody A00166 work for IHC with leukemic t-cell?
Verified Customer
Verified customer
Asked: 2019-11-15
Answer
According to the expression profile of leukemic t-cell, CDK2 is highly expressed in leukemic t-cell. So, it is likely that anti-cdk2 antibody A00166 will work for IHC with leukemic t-cell.
Boster Scientific Support
Answered: 2019-11-15
Question
My question regarding product A00166, anti-cdk2 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-10-07
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00166 anti-cdk2 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-10-07
Question
Is a blocking peptide available for product anti-cdk2 antibody (A00166)?
Verified Customer
Verified customer
Asked: 2019-05-15
Answer
We do provide the blocking peptide for product anti-cdk2 antibody (A00166). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-05-15
Question
Will A00166 anti-cdk2 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-05-03
Answer
You can see on the product datasheet, A00166 anti-cdk2 antibody as been validated on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-05-03
Question
I was wanting to use your anti-cdk2 antibody for IHC for human leukemic t-cell on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human leukemic t-cell identification?
L. Anderson
Verified customer
Asked: 2018-10-11
Answer
You can see on the product datasheet, A00166 anti-cdk2 antibody has been validated for IP, IHC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human leukemic t-cell in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-10-11
Question
Is there a BSA free version of anti-cdk2 antibody A00166 available?
Verified Customer
Verified customer
Asked: 2018-02-13
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-cdk2 antibody A00166 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-02-13
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for leukemic t-cell using anti-cdk2 antibody A00166. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2018-01-25
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-01-25
Question
My boss were satisfied with the WB result of your anti-cdk2 antibody. However we have observed positive staining in leukemic t-cell cytoplasm using this antibody. Is that expected? Could you tell me where is CDK2 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2017-07-05
Answer
From literature, leukemic t-cell does express CDK2. Generally CDK2 expresses in cytoplasm, cytoskeleton, microtubule. Regarding which tissues have CDK2 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18691976
Leukemic T-cell, Pubmed ID: 19690332
Placenta, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2017-07-05
Question
I am looking for using your anti-cdk2 antibody for cell division studies. Has this antibody been tested with western blotting on hela whole cell lysates? We would like to see some validation images before ordering.
W. Thomas
Verified customer
Asked: 2017-03-15
Answer
We appreciate your inquiry. This A00166 anti-cdk2 antibody is tested on hela whole cell lysates. It is guaranteed to work for IP, IHC, WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2017-03-15
Question
I see that the anti-cdk2 antibody A00166 works with IHC, what is the protocol used to produce the result images on the product page?
E. Bhatt
Verified customer
Asked: 2016-03-22
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2016-03-22
Question
We ordered your anti-cdk2 antibody for IP on left lobe of thyroid gland a few months ago. I am using human, and We intend to use the antibody for WB next. I would like examining left lobe of thyroid gland as well as leukemic t-cell in our next experiment. Do you have any suggestion on which antibody would work the best for WB?
G. Johnson
Verified customer
Asked: 2015-02-09
Answer
I have checked the website and datasheets of our anti-cdk2 antibody and I see that A00166 has been validated on human in both IP and WB. Thus A00166 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2015-02-09
Question
We are currently using anti-cdk2 antibody A00166 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on feline tissues as well?
S. Li
Verified customer
Asked: 2014-05-06
Answer
The anti-cdk2 antibody (A00166) has not been validated for cross reactivity specifically with feline tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2014-05-06
Question
We have been able to see staining in human leukemic t-cell. What should we do? Is anti-cdk2 antibody supposed to stain leukemic t-cell positively?
J. Collins
Verified customer
Asked: 2013-03-20
Answer
According to literature leukemic t-cell does express CDK2. According to Uniprot.org, CDK2 is expressed in left lobe of thyroid gland, placenta, cervix carcinoma, leukemic t-cell, among other tissues. Regarding which tissues have CDK2 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18691976
Leukemic T-cell, Pubmed ID: 19690332
Placenta, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2013-03-20
Question
Our lab want to know about to test anti-cdk2 antibody A00166 on human leukemic t-cell for research purposes, then I may be interested in using anti-cdk2 antibody A00166 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
E. Moore
Verified customer
Asked: 2013-03-14
Answer
The products we sell, including anti-cdk2 antibody A00166, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2013-03-14
