Click image to see more details
Product Info Summary
| SKU: | A14160 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, ICC, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-Cadherin-22 CDH22 Antibody
View all Cadherin-22 Antibodies
SKU/Catalog Number
A14160
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Cadherin-22 CDH22 Antibody (Catalog# A14160). Tested in WB, IHC-P, IF, ICC, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Cadherin-22 CDH22 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A14160)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human CDH22. Immunogen sequence location: 111-160
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A14160 is reactive to CDH22 in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
72 kDa
Calculated molecular weight
89091 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A14160 is guaranteed for ELISA, IF, IHC-P, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western Blot, 1:500 - 1:2000
Immunohistochemistry, 1:100 - 1:300
Immunofluorescence, 1:200 - 1:1000
ELISA, 1:10000
Not yet tested in other applications.
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of lysates from rat brain cells, using CDH22 Antibody. The lane on the right is blocked with the synthesized peptide.
Click image to see more details
Immunohistochemistry analysis of paraffin-embedded human brain tissue, using CDH22 Antibody. The picture on the right is blocked with the synthesized peptide.
Click image to see more details
Immunofluorescence analysis of NIH/3T3 cells, using CDH22 Antibody. The picture on the right is blocked with the synthesized peptide.
Protein Target Info & Infographic
Gene/Protein Information For CDH22 (Source: Uniprot.org, NCBI)
Gene Name
CDH22
Full Name
Cadherin-22
Weight
89091 MW
Alternative Names
CDH22;C20orf25;Cadherin-22;Pituitary and brain cadherin;PB-cadherin Cdh22|cadherin 22|cadherin-22|PB-cadherin|pituitary and brain cadherin
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CDH22, check out the CDH22 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CDH22: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Cadherin-22 CDH22 Antibody (A14160)
Loading publications
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-Cadherin-22 CDH22 Antibody?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Anti-Cadherin-22 CDH22 Antibody
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
7 Customer Q&As for Anti-Cadherin-22 CDH22 Antibody
Question
We are currently using anti-CDH22 antibody A14160 for rat tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on zebrafish tissues as well?
Verified Customer
Verified customer
Asked: 2019-10-31
Answer
The anti-CDH22 antibody (A14160) has not been validated for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-10-31
Question
My question regarding product A14160, anti-CDH22 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-09-10
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A14160 anti-CDH22 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-09-10
Question
Would anti-CDH22 antibody A14160 work on monkey WB with brain?
Verified Customer
Verified customer
Asked: 2019-08-07
Answer
Our lab technicians have not validated anti-CDH22 antibody A14160 on monkey. You can run a BLAST between monkey and the immunogen sequence of anti-CDH22 antibody A14160 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated monkey samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in monkey brain in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-08-07
Question
Would anti-CDH22 antibody A14160 work for IF with cerebellar vermis?
Verified Customer
Verified customer
Asked: 2018-08-16
Answer
According to the expression profile of cerebellar vermis, CDH22 is highly expressed in cerebellar vermis. So, it is likely that anti-CDH22 antibody A14160 will work for IF with cerebellar vermis.
Boster Scientific Support
Answered: 2018-08-16
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for cerebellar vermis using anti-CDH22 antibody A14160. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2018-03-14
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-03-14
Question
I see that the anti-CDH22 antibody A14160 works with IF, what is the protocol used to produce the result images on the product page?
A. Mitchell
Verified customer
Asked: 2015-01-07
Answer
You can find protocols for IF on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2015-01-07
Question
I was wanting to use your anti-CDH22 antibody for IF for human cerebellar vermis on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human cerebellar vermis identification?
E. Parker
Verified customer
Asked: 2014-07-31
Answer
You can see on the product datasheet, A14160 anti-CDH22 antibody has been validated for IF, IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human cerebellar vermis in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2014-07-31
