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Product Info Summary
| SKU: | PB9872 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-MDMX/MDM4 Antibody Picoband®
SKU/Catalog Number
PB9872
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-MDMX/MDM4 Antibody Picoband® catalog # PB9872. Tested in IHC, WB applications. This antibody reacts with Human, Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-MDMX/MDM4 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9872)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human MDMX, different from the related mouse sequence by two amino acids, and from the related rat sequence by three amino acids.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
PB9872 is reactive to MDM4 in Human, Mouse
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
75 kDa
Calculated molecular weight
54864 MW
Background of MDMX
Protein Mdm4 is a protein that in humans is encoded by the MDM4 gene. This gene encodes a nuclear protein that contains a p53 binding domain at the N-terminus and a RING finger domain at the C-terminus, and shows structural similarity to p53-binding protein MDM2. Both proteins bind the p53 tumor suppressor protein and inhibit its activity, and have been shown to be overexpressed in a variety of human cancers. However, unlike MDM2 which degrades p53, this protein inhibits p53 by binding its transcriptional activation domain. This protein also interacts with MDM2 protein via the RING finger domain, and inhibits the latter's degradation. So this protein can reverse MDM2-targeted degradation of p53, while maintaining suppression of p53 transactivation and apoptotic functions. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9872 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Western blot, 0.1-0.5μg/ml, Human, Mouse
Positive Control
WB: mouse testis tissue, 22RV1 whole cell
IHC: human lung cancer tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of MDMX using anti-MDMX antibody (PB9872).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: mouse testis tissue lysates,
Lane 2: 22RV1 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MDMX antigen affinity purified polyclonal antibody (Catalog # PB9872) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MDMX at approximately 75 kDa. The expected band size for MDMX is at 55 kDa.
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Figure 2. IHC analysis of MDMX using anti-MDMX antibody (PB9872).
MDMX was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-MDMX Antibody (PB9872) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For MDM4 (Source: Uniprot.org, NCBI)
Gene Name
MDM4
Full Name
Protein Mdm4
Weight
54864 MW
Superfamily
MDM2/MDM4 family
Alternative Names
Protein Mdm4;Double minute 4 protein;Mdm2-like p53-binding protein;Protein Mdmx;p53-binding protein Mdm4;MDM4;MDMX; MDM4 BMFS6, HDMX, MDMX, MRP1 MDM4 regulator of p53 protein Mdm4|MDM4 protein variant G|MDM4 protein variant Y|MDM4, p53 regulator|MDM4-related protein 1|Mdm4 p53 binding protein homolog|double minute 4, human homolog of; p53-binding protein|mdm2-like p53-binding protein|protein Mdmx
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MDM4, check out the MDM4 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MDM4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-MDMX/MDM4 Antibody Picoband® (PB9872)
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No publications found for PB9872
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Customer Q&As
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6 Customer Q&As for Anti-MDMX/MDM4 Antibody Picoband®
Question
I was wanting to use your anti-MDMX/MDM4 antibody for IHC for human colon tumor on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human colon tumor identification?
Verified Customer
Verified customer
Asked: 2020-03-19
Answer
You can see on the product datasheet, PB9872 anti-MDMX/MDM4 antibody has been validated for IHC, WB on human, mouse tissues. We have an innovator award program that if you test this antibody and show it works in human colon tumor in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-03-19
Question
you antibody to test anti-MDMX/MDM4 antibody PB9872 on human colon tumor for research purposes, then I may be interested in using anti-MDMX/MDM4 antibody PB9872 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-09-23
Answer
The products we sell, including anti-MDMX/MDM4 antibody PB9872, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-09-23
Question
We are currently using anti-MDMX/MDM4 antibody PB9872 for human tissue, and we are content with the IHC results. The species of reactivity given in the datasheet says human, mouse. Is it true that the antibody can work on canine tissues as well?
S. Anderson
Verified customer
Asked: 2019-09-05
Answer
The anti-MDMX/MDM4 antibody (PB9872) has not been tested for cross reactivity specifically with canine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-09-05
Question
Would anti-MDMX/MDM4 antibody PB9872 work on primate WB with gastric mucosa?
Verified Customer
Verified customer
Asked: 2019-08-07
Answer
Our lab technicians have not validated anti-MDMX/MDM4 antibody PB9872 on primate. You can run a BLAST between primate and the immunogen sequence of anti-MDMX/MDM4 antibody PB9872 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated primate samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in primate gastric mucosa in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-08-07
Question
Is a blocking peptide available for product anti-MDMX/MDM4 antibody (PB9872)?
Verified Customer
Verified customer
Asked: 2018-12-24
Answer
We do provide the blocking peptide for product anti-MDMX/MDM4 antibody (PB9872). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2018-12-24
Question
See below the WB image, lot number and protocol we used for colon tumor using anti-MDMX/MDM4 antibody PB9872. Please let me know if you require anything else.
S. Johnson
Verified customer
Asked: 2013-07-29
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2013-07-29
