Product Info Summary
SKU: | M01899 |
---|---|
Size: | 100 μl |
Reactive Species: | Human |
Host: | Rabbit |
Application: | IP, WB |
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Product info
Product Name
Anti-Cdc25B Rabbit Monoclonal Antibody
SKU/Catalog Number
M01899
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Cdc25B Rabbit Monoclonal Antibody catalog # M01899. Tested in WB, IP applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Cdc25B Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01899)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
HIH-3
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Cdc25B
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M01899 is reactive to CDC25B in Human
Applications
M01899 is guaranteed for IP, WB Boster Guarantee
Observed Molecular Weight
270 kDa
Calculated molecular weight
64987 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IP 1:50
Validation Images & Assay Conditions
![m01899 wb7 m01899 wb7](https://www.bosterbio.com/media/catalog/product/m/0/m01899-wb7.jpg)
Click image to see more details
All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
![m01899 wb8 m01899 wb8](https://www.bosterbio.com/media/catalog/product/m/0/m01899-wb8.jpg)
Click image to see more details
All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
![m01899 wb m01899 wb](https://www.bosterbio.com/media/catalog/product/m/0/m01899-wb.jpg)
Click image to see more details
Western blot analysis of Cdc25B expression in U937 cell lysate.
Protein Target Info & Infographic
Gene/Protein Information For CDC25B (Source: Uniprot.org, NCBI)
Gene Name
CDC25B
Full Name
M-phase inducer phosphatase 2
Weight
64987 MW
Superfamily
MPI phosphatase family
Alternative Names
CDC25B; CDC25HU2; cell division cycle 25 homolog B (S. cerevisiae); cell division cycle 25 homolog B (S. pombe); cell division cycle 25B; Dual specificity phosphatase Cdc25B; EC 3.1.3.48; M-phase inducer phosphatase 2 CDC25B cell division cycle 25B M-phase inducer phosphatase 2|CDC25 homolog B|dual specificity phosphatase Cdc25B
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CDC25B, check out the CDC25B Infographic
![CDC25B infographic](/media/images/gene-infographic-example.jpg)
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CDC25B: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Cdc25B Rabbit Monoclonal Antibody (M01899)
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