Product Info Summary
SKU: | EKC1980 |
---|---|
Size: | 1 kit, containing two 96-well plates and all necessary reagents |
Reactive Species: | Human, Mouse, Rat |
Application: | ELISA |
Sample Types: | Cell lines |
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Product info
Product Name
CDC25B (Phospho-Ser323) Colorimetric Cell-Based ELISA Kit
SKU/Catalog Number
EKC1980
Size
1 kit, containing two 96-well plates and all necessary reagents
Description
The CDC25B (Phospho-Ser323) Cell-Based ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can monitor CDC25B (Phospho-Ser323) protein expression profile in cells. The kit can be used for measuring the relative amounts of CDC25B (Phospho-Ser323) in cultured cells as well as screening for the effects that various treatments, inhibitors (ie. siRNA or chemicals), or activators have on CDC25B (Phospho-Ser323).
Storage & Handling
Store at 4°C for up to 6 months.
Cite This Product
CDC25B (Phospho-Ser323) Colorimetric Cell-Based ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # EKC1980)
Clonality of Antibodies
See Datasheet for details
Assay Range
> 5000 cells/well
Reactive Species
EKC1980 is reactive to CDC25B in Human, Mouse, Rat samples
Validated Sample Types
Cell lines
Application Guarantee
EKC1980 is guaranteed for ELISA in Human, Mouse, Rat by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Kit Components
Reagent | Quantity | Container |
---|---|---|
96-Well Cell Culture Clear-Bottom Microplate | 2 Plates | - |
10x TBS | 24 ml (10x) | Clear |
Quenching Buffer | 24 ml (1x) | Clear |
Blocking Buffer | 50 ml (1x) | Clear |
15x Wash Buffer | 50 ml (15x) | Clear |
100x Anti-CDC25B Phospho-Ser323 Antibody (Rabbit Polyclonal) | 60 µl (100x) | Red |
100x Anti-CDC25B Antibody (Rabbit Polyclonal) | 60 µl (100x) | Purple |
100x Anti-GAPDH Antibody (Mouse Monoclonal) | 60 µl (100x) | Green |
HRP-Conjugated Anti-Rabbit IgG Antibody | 12ml (1x) | Glass |
HRP-Conjugated Anti-Mouse IgG Antibody | 12ml (1x) | Glass |
Primary Antibody Diluent | 12 ml (1x) | Clear |
Ready-to-Use Substrate | 12 ml (1x) | Brown |
Stop Solution | 12 ml (1x) | Clear |
Crystal Violet Solution | 12ml (1x) | Glass |
SDS Solution | 24 ml (1x) | Clear |
Adhesive Plate Seals | 2 Seals | - |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate reader able to measure absorbance at 450 nm and/or 595 nm for Crystal Violet Cell Staining (Optional)
- Micropipettes with capability of measuring volumes ranging from 1 ul to 1 ml
- 37% formaldehyde (Sigma Cat# F-8775) or formaldehyde from other sources
- Deionized or sterile water
- Squirt bottle, manifold dispenser, multichannel pipette reservoir or automated microplate washer
- Graph paper or computer software capable of generating or displaying logarithmic functions
- Absorbent papers or vacuum aspirator
- Test tubes or microfuge tubes capable of storing 1 ml
- Orbital shaker
- Poly-L-Lysine (Sigma Cat# P4832 for suspension cells)
Data Examples, Quality Control Data
Click image to see more details
Boster Kit Box
Click image to see more details
Western blot analysis of extracts from NIH-3T3 cells treated with PMA 125ng/ml 30'
Protein Target Info & Infographic
Gene/Protein Information For CDC25B (Source: Uniprot.Org, NCBI)
Gene Name
CDC25B
Full Name
M-phase inducer phosphatase 2
Weight
64987 MW
Superfamily
MPI phosphatase family
Alternative Names
CDC25HU2;CDC25M2;Dual specificity phosphatase Cdc25B;M-phase inducer phosphatase 2;MPIP2 CDC25B cell division cycle 25B M-phase inducer phosphatase 2|CDC25 homolog B|dual specificity phosphatase Cdc25B
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on CDC25B, check out the CDC25B Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for CDC25B: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For CDC25B (Phospho-Ser323) Colorimetric Cell-Based ELISA Kit (EKC1980)
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