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Product Info Summary
| SKU: | A01794-3 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, WB |
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Product info
Product Name
Anti-CD98/Slc3a2 Antibody Picoband®
SKU/Catalog Number
A01794-3
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-CD98/Slc3a2 Antibody Picoband® catalog # A01794-3. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-CD98/Slc3a2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01794-3)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived mouse CD98/Slc3a2 recombinant protein (Position: K35-Q497).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A01794-3 is reactive to Slc3a2 in Mouse, Rat
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
80-90 kDa
Calculated molecular weight
67.994kDa
Background of CD98
4F2 cell-surface antigen heavy chain is a protein that in humans is encoded by the SLC3A2 (solute carrier family 3 member 2) gene. Predicted to enable amino acid transmembrane transporter activity and double-stranded RNA binding activity. Predicted to be involved in carboxylic acid transport and viral entry into host cell. Predicted to act upstream of or within response to exogenous dsRNA. Located in apical pole of neuron. Is active in synapse. Is expressed in several structures, including alimentary system; brain; genitourinary system; hemolymphoid system gland; and spinal cord. Orthologous to human SLC3A2 (solute carrier family 3 member 2).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01794-3 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml/ml, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/ml/1x10^6 cells, Mouse
ELISA, 0.1-0.5 μg/ml/ml, Mouse
Positive Control
WB: rat pancreas tissue, rat kidney tissue, mouse pancreas tissue, mouse kidney tissue, mouse testis tissue, mouse NIH/3T3 whole cell
FCM: RAW2647 cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of CD98/Slc3a2 using anti-CD98/Slc3a2 antibody (A01794-3).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat pancreas tissue lysates,
Lane 2: rat kidney tissue lysates,
Lane 3: mouse pancreas tissue lysates,
Lane 4: mouse kidney tissue lysates,
Lane 5: mouse testis tissue lysates,
Lane 6: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD98/Slc3a2 antigen affinity purified polyclonal antibody (Catalog # A01794-3) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD98/Slc3a2 at approximately 80-90 kDa. The expected band size for CD98/Slc3a2 is at 68 kDa.
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Figure 2. Flow Cytometry analysis of RAW264.7 cells using anti-CD98/Slc3a2 antibody (A01794-3).
Overlay histogram showing RAW264.7 cells stained with A01794-3 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-CD98/Slc3a2 Antibody (A01794-3, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For Slc3a2 (Source: Uniprot.org, NCBI)
Gene Name
Slc3a2
Full Name
4F2 cell-surface antigen heavy chain
Weight
67.994kDa
Superfamily
SLC3A transporter family
Alternative Names
Protein CBFA2T1; Cyclin-D-related protein; Eight twenty one protein; Protein ETO; Protein MTG8; Zinc finger MYND domain-containing protein 2; RUNX1T1; AML1T1; CBFA2T1; CDR; ETO; MTG8; ZMYND2 SLC3A2 4F2, 4F2HC, 4T2HC, CD98, CD98HC, MDU1, NACAE solute carrier family 3 member 2 4F2 cell-surface heavy chain|CD98 heavy chain| defined by 4F2, heavy chain| by antibodies 4F2, TRA1.10, TROP4, and T43|lymphocyte activation 4F2 large subunit| 44D7|solute carrier family 3 (activators of dibasic and neutral amino acid transport), member 2|solute carrier family 3 (amino acid transporter heavy chain), member 2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Slc3a2, check out the Slc3a2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Slc3a2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CD98/Slc3a2 Antibody Picoband® (A01794-3)
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