Product Info Summary
SKU: | A01794-1 |
---|---|
Size: | 100μl |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | ELISA, WB |
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Product info
Product Name
Anti-CD98 SLC3A2 Antibody
SKU/Catalog Number
A01794-1
Size
100μl
Form
Liquid
Description
Boster Bio Anti-CD98 SLC3A2 Antibody catalog # A01794-1. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CD98 SLC3A2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01794-1)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from the C-terminal region of human SLC3A2 at amino acid range 491-540
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A01794-1 is reactive to SLC3A2 in Human, Mouse, Rat
Applications
A01794-1 is guaranteed for ELISA, WB Boster Guarantee
Observed Molecular Weight
39 kDa
Calculated molecular weight
67994 MW
Background of CD98
Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3') and activates the promoters of the genes for some small nuclear RNAs (snRNA) and of genes such as those for histone H2B and immunoglobulins. Modulates transcription transactivation by NR3C1, AR and PGR By similarity. In case of human herpes simplex virus (HSV) infection, POU2F1 forms a multiprotein-DNA complex with the viral transactivator protein VP16 and HCFC1 thereby enabling the transcription of the viral immediate early genes.
Prefontaine G.G., Walther R., Giffin W. J. Biol. Chem. 274:26713-26719(1999)
Mahajan S.S., Wilson A.C. Mol. Cell. Biol. 20:919-928(2000)
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
ELISA 1:10000
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of HEB cells using CD98 Polyclonal Antibody. Secondary antibody was diluted at 1:20000
Protein Target Info & Infographic
Gene/Protein Information For SLC3A2 (Source: Uniprot.org, NCBI)
Gene Name
SLC3A2
Full Name
4F2 cell-surface antigen heavy chain
Weight
67994 MW
Superfamily
SLC3A transporter family
Alternative Names
4F2; 4F2hc; 4T2HC; antigen identified by monoclonal antibodies 4F2, TRA1.10, TROP4, and T43,4F2HC; CD98 antigen; CD98 heavy chain; CD98; CD98HC; CH98hc; FRP-1; heavy chain; Lymphocyte activation antigen 4F2 large subunit; MDU1antigen defined by monoclonal 4F2, heavy chain; monoclonal 44D7; NACAE4F2 cell-surface antigen heavy chain; SLC3A2; solute carrier family 3 (activators of dibasic and neutral amino acidtransport), member 2,4F2 heavy chain antigen SLC3A2 4F2, 4F2HC, 4T2HC, CD98, CD98HC, MDU1, NACAE solute carrier family 3 member 2 4F2 cell-surface antigen heavy chain|CD98 heavy chain|antigen defined by monoclonal antibody 4F2, heavy chain|antigen identified by monoclonal antibodies 4F2, TRA1.10, TROP4, and T43|lymphocyte activation antigen 4F2 large subunit|monoclonal antibody 44D7|solute carrier family 3 (activators of dibasic and neutral amino acid transport), member 2|solute carrier family 3 (amino acid transporter heavy chain), member 2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SLC3A2, check out the SLC3A2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SLC3A2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CD98 SLC3A2 Antibody (A01794-1)
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Customer Q&As
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4 Customer Q&As for Anti-CD98 SLC3A2 Antibody
Question
We are currently using anti-CD98 antibody A01794-1 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on pig tissues as well?
Verified Customer
Verified customer
Asked: 2020-01-29
Answer
The anti-CD98 antibody (A01794-1) has not been tested for cross reactivity specifically with pig tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-01-29
Question
Would anti-CD98 antibody A01794-1 work on zebrafish WB with melanoma?
Verified Customer
Verified customer
Asked: 2020-01-13
Answer
Our lab technicians have not tested anti-CD98 antibody A01794-1 on zebrafish. You can run a BLAST between zebrafish and the immunogen sequence of anti-CD98 antibody A01794-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated zebrafish samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in zebrafish melanoma in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-01-13
Question
Would A01794-1 anti-CD98 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-09-09
Answer
You can see on the product datasheet, A01794-1 anti-CD98 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-09-09
Question
My question regards to test anti-CD98 antibody A01794-1 on mouse plasma for research purposes, then I may be interested in using anti-CD98 antibody A01794-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-05-13
Answer
The products we sell, including anti-CD98 antibody A01794-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-05-13