Product Info Summary
SKU: | A02982 |
---|---|
Size: | 500ug |
Reactive Species: | Human, Chimpanzee |
Host: | Rabbit |
Application: | IF, WB |
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Product info
Product Name
Anti-CD97 antigen CD97 Antibody
SKU/Catalog Number
A02982
Size
500ug
Form
Lyophilized
Description
Boster Bio Anti-CD97 antigen CD97 Antibody (Catalog # A02982). Tested in IF, WB applications. This antibody reacts with Human, Chimpanzee.
Storage & Handling
Store vial at 4°C prior to restoration. For extended storage aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.?Expiration date is one (1) year from date of opening. (Ship on dry ice.)
Cite This Product
Anti-CD97 antigen CD97 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02982)
Host
Rabbit
Contents
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide
Clonality
Polyclonal
Isotype
IgG
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein corresponding to amino acids 1-512 (extracellular domain) of mouse CD97 protein.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
beta-Actin antibody is human, mouse, rat, rabbit, chicken, zebrafish and drosophila reactive.
Reactive Species
A02982 is reactive to CD97 in Human, Chimpanzee
Applications
A02982 is guaranteed for IF, WB Boster Guarantee
Observed Molecular Weight
68 kDa
Calculated molecular weight
117849 MW
Background of Cd97
This antibody is suitable for Cancer, Immunology and Nuclear Signaling research. CD97 is a membrane antigen that is either constitutively expressed or induced by activation on cells of the immune system including colon, thyroid, stomach, and esophagus. One high affinity ligand that has been identified for CD97 is the integrin alpha 5 beta 1. CD97 engagement of endothelial alpha5 beta 1 results in increased angiogenesis. Signals generated through CD97 are thought to result in Rho activation and the regulation of motility. Anti-CD97 is ideal for researchers interested in cancer, angiogenesis, and inflammation.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 100 µL
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ELISA: 1:10,000 - 1:50,000
IF Microscopy: 1:1,000 - 1:5,000
WB: 1:1,000 - 1:5,000
Validation Images & Assay Conditions
Click image to see more details
Immunofluorescence Microscopy using Protein A purified anti-CD97 antibody shows staining of Fc-CD97-(5EGF) (panel D) in transfected COS cells.Panel A and C shows similar staining using pre-immune serum.Panel A and B show staining of mock transfected COS cells (no vector).A 1:2,500 dilution of the primary antibody was used.Personal Communication.Yvona Ward.NIH, NCI, CCR, Bethesda, MD.
Click image to see more details
Figure 1. Western blot analysis of CD97 using anti-CD97 antibody (A02982).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD97 antigen affinity purified polyclonal antibody (Catalog # A02982) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system.
Protein Target Info & Infographic
Gene/Protein Information For CD97 (Source: Uniprot.org, NCBI)
Gene Name
CD97
Full Name
CD97 antigen
Weight
117849 MW
Superfamily
G-protein coupled receptor 2 family
Alternative Names
CD97
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CD97, check out the CD97 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CD97: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CD97 antigen CD97 Antibody (A02982)
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