Key=store_id
Key=entity_id, value=42910
Key=attribute_set_id, value=4
Key=type_id, value=simple
Key=sku, value=A02982
Key=has_options, value=1
Key=required_options, value=1
Key=created_at, value=2018-01-31 04:47:49
Key=updated_at, value=2024-10-30 05:01:42
Key=mst_search_weight, value=0
Key=status, value=Enabled
Key=name, value=Anti-CD97 antigen CD97 Antibody
Key=cj_search_index
Key=publication_count, value=0
Key=qty_in_warehouses
Key=is_recurring, value=No
Key=visibility, value=Catalog, Search
Key=tax_class_id, value=Taxable Goods
Key=conjugate, value=Biotin
Key=featured, value=No
Key=featured_image, value=Yes
Key=fb_product, value=
Key=custom_product_page, value=No
Key=free_shipping, value=No
Key=template, value=antibodies
Key=host, value=Rabbit
Key=googleshopping_exclude, value=No
Key=yoast_keyword_score, value=-1369
Key=yoast_content_score, value=90
Key=price, value=699.0000
Key=weight, value=1.0000
Key=meta_title, value=Anti-CD97 antigen CD97 Antibody
Key=meta_description, value=Boster Bio Anti-CD97 antigen CD97 Antibody (Catalog # A02982). Tested in IF, WB applications. This antibody reacts with Human, Chimpanzee. Supplied as 500ug in Lyophilized form antibody.
Key=image, value=/A/0/A02982-CD97-WB-test-1.jpg
Key=small_image, value=/A/0/A02982-CD97-WB-test-1.jpg
Key=thumbnail, value=/A/0/A02982-CD97-WB-test-1.jpg
Key=url_key, value=anti-cd97-antibody-a02982-boster
Key=url_path, value=anti-cd97-antibody-a02982-boster
Key=options_container, value=Product Info Column
Key=image_label, value=Figure 1. Western blot analysis of CD97 using anti-CD97 antibody (A02982).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample unde
Key=small_image_label, value=Figure 1. Western blot analysis of CD97 using anti-CD97 antibody (A02982).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample unde
Key=thumbnail_label, value=Figure 1. Western blot analysis of CD97 using anti-CD97 antibody (A02982).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample unde
Key=msrp_display_actual_price_type, value=
Key=gift_message_available, value=No
Key=size, value=500ug
Key=protein_name, value=Gamma-aminobutyric acid type B receptor subunit 1
Key=gene_full_name, value=Ubiquitin-like modifier-activating enzyme 1
Key=uniprot_id, value=Q9Z0M6
Key=reconstitution, value=Restore with deionized water (or equivalent) for reconstitution volume of 100 µL
Key=sensitivity
Key=applications, value=IF, WB
Key=reactivity, value=Human, Chimpanzee
Key=form, value=Lyophilized
Key=concentration, value=5.0 mg/mL by UV absorbance at 280 nm
Key=cross_reactivity, value=beta-Actin antibody is human, mouse, rat, rabbit, chicken, zebrafish and drosophila reactive.
Key=clone_number
Key=predicted_reactivity, value=Mouse, Rat
Key=source_company, value=Rockland 200-401-908; Cost: 387.8
Key=isotype, value=IgG
Key=molecular_weight, value=117849 MW
Key=sample_size_available, value=25ug for $99, contact us for details
Key=related_product_skus, value=M02810
Key=product_category, value=Primary Antibodies
Key=research_category, value=Cell Type Markers, G Protein Signaling, Hematopoietic Progenitors, Immunology, More Gpcr, Neuroscience, Neurotransmission, Receptors / Channels, Signal Transduction, Signaling Pathway, Stem Cells
Key=clonality, value=Polyclonal
Key=swatch_image, value=no_selection
Key=rating_value, value=100
Key=review_count, value=3
Key=mp_exclude_sitemap, value=No
Key=yoast_facebook_image, value=no_selection
Key=yoast_twitter_image, value=no_selection
Key=cj_related_products, value=Anaplasia:A01493-1^Clonic Convulsion:A15261|A15261-1|PROTO75564^Long-term Memory:EK0307-PO|EZ0308
Key=description, value=Boster Bio Anti-CD97 antigen CD97 Antibody (Catalog # A02982). Tested in IF, WB applications. This antibody reacts with Human, Chimpanzee.
Key=short_description, value=Boster Bio Anti-CD97 antigen CD97 Antibody (Catalog # A02982). Tested in IF, WB applications. This antibody reacts with Human, Chimpanzee.
Key=meta_keyword, value=Anti-CD97 antigen CD97 Antibody
Key=contents, value=0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide
Key=background, value=This antibody is suitable for Cancer, Immunology and Nuclear Signaling research. CD97 is a membrane antigen that is either constitutively expressed or induced by activation on cells of the immune system including colon, thyroid, stomach, and esophagus. One high affinity ligand that has been identified for CD97 is the integrin alpha 5 beta 1. CD97 engagement of endothelial alpha5 beta 1 results in increased angiogenesis. Signals generated through CD97 are thought to result in Rho activation and the regulation of motility. Anti-CD97 is ideal for researchers interested in cancer, angiogenesis, and inflammation.
Key=gene_name, value=CD97
Key=synonyms, value=CD97, CD97 antigen, CD97 molecule, Leukocyte antigen CD97, CD97 antigen subunit alpha, CD97 antigen subunit beta
Key=application_details, value=ELISA: 1:10,000 - 1:50,000
IF Microscopy: 1:1,000 - 1:5,000
WB: 1:1,000 - 1:5,000
Key=subcellular_localization, value= Cytoplasm. Mitochondrion. Nucleus.
Key=tissue_specificity, value= Detected in erythrocytes (at protein level). Ubiquitous.
Key=protein_function, value= Catalyzes the first step in ubiquitin conjugation to mark cellular proteins for degradation through the ubiquitin- proteasome system (PubMed:1606621, PubMed:1447181). Activates ubiquitin by first adenylating its C-terminal glycine residue with ATP, and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding a ubiquitin-E1 thioester and free AMP (PubMed:1447181). Essential for the formation of radiation- induced foci, timely DNA repair and for response to replication stress. Promotes the recruitment of TP53BP1 and BRCA1 at DNA damage sites (PubMed:22456334).
Key=application_notes, value=This Protein A purified antibody has been tested for use in immunofluorescence microscopy and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 81 kDa in size corresponding to isoform 1 of CD97 protein by western blotting in the appropriate cell lysate or extract.
Key=competitor_equivalent_skus, value=sc 47555|sc 47554|sc 292773|sc 47552|sc 271821|sc 53555
Key=immunogen, value=This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein corresponding to amino acids 1-512 (extracellular domain) of mouse CD97 protein.
Key=purification, value=This Protein A purified antibody is directed against the mouse CD97 protein. The product was protein A purified from monospecific antiserum.  A BLAST analysis was used to suggest cross-reactivity with CD97 proteins from mouse (100%) and rat (74%).  Only 49% homology is noted for the human homologue.  Reactivity against CD97 from other sources is not known.
Key=storage, value=Store vial at 4°C prior to restoration. For extended storage aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.?Expiration date is one (1) year from date of opening. (Ship on dry ice.)
Key=custom_attribute_1, value=68 kDa
Key=description_before_attributes, value=

Introduction

CD3ε is a 20kD chain, which together with CD3λ, CD3δ, and CD3ζ, and a T cell receptor (α/β or γ/) form the CD3/T-cell receptor complex. It is a specific marker for T lymphocytes, NK T cells, and some thymocytes. Crosslinking of TCR initiates an intracellular signaling cascade resulting in cellular activation and proliferation. The OKT3 antibody has been reported to have potent immunosuppressive properties in vivo and has been proved effective in the treatment of renal, heart, and liver allograft rejection.

This antibody is routinely tested by flow cytometric analysis. Flow cytometry and other applications were tested during antibody development or are reported in the literature.

Application Information

Each lot of this antibody has been quality control tested by flow cytometric analysis of human PBMCs. For flow cytometric staining, the recommended use of this antibody is ≤ 0.5µg per 1x106 cells in 100µl of staining volume followed by a secondary florescent conjugated anti-mouse antibody. However, it is strongly suggested that the antibody reactivity be empirically titrated for optimal performance in the application of interest.

Key=options
Key=media_gallery
Key=extension_attributes
Key=tier_price
Key=tier_price_changed
Key=category_ids
Key=quantity_and_stock_status
Key=is_salable
Key=website_ids
Key=request_path
Key=rating_summary
Key=_cache_instance_store_filter
Key=media_gallery_images