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Product Info Summary
| SKU: | A15261 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-JRK Antibody Picoband®
SKU/Catalog Number
A15261
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-JRK Antibody Picoband® catalog # A15261. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-JRK Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A15261)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human JRK, which shares 93.8% and 87.5% amino acid (aa) sequence identity with mouse and rat JRK, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A15261 is reactive to JRK in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
65 kDa
Calculated molecular weight
24145 MW
Background of JRK
This gene encodes a conserved protein that is similar to DNA-binding proteins, such as major centromere autoantigen B (CENPB). Inactivation of the related gene in mice resulted in epileptic seizures. Childhood Absence Epilepsy (CAE) has been mapped to the same chromosomal location (8q24.3) as this gene. Alternative splicing results in multiple transcript variants.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A15261 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5μg/ml, Human
Positive Control
WB: human A375 whole cell, human A431 whole cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of JRK using anti-JRK antibody (A15261).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions.
Lane 1: human A375 whole cell lysates,
Lane 2: human A431 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-JRK antigen affinity purified polyclonal antibody (Catalog # A15261) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for JRK at approximately 65KD. The expected band size for JRK is at 65KD.
Protein Target Info & Infographic
Gene/Protein Information For JRK (Source: Uniprot.org, NCBI)
Gene Name
JRK
Full Name
Jerky protein homolog
Weight
24145 MW
Superfamily
tigger transposable element derived protein family
Alternative Names
RING finger protein 186;RNF186; JRK JH8, jerky Jrk helix-turn-helix protein jerky protein homolog|Jrk homolog|jerky homolog
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on JRK, check out the JRK Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for JRK: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-JRK Antibody Picoband® (A15261)
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