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Product Info Summary
| SKU: | M02982 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-CD97 Monoclonal Antibody
SKU/Catalog Number
M02982
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-CD97 Monoclonal Antibody catalog # M02982. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CD97 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02982)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
ADEF-3
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human CD97 Receptor potentially involved in both adhesion and signaling processes early after leukocyte activation. Plays an essential role in leukocyte migration.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M02982 is reactive to CD97 in Human, Mouse
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
28 kDa
Calculated molecular weight
45333 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M02982 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:50
FC 1:50
Positive Control
WB: U937 cell
Validation Images & Assay Conditions
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Western blot analysis of CD97 expression in U937 cell lysate.
Protein Target Info & Infographic
Gene/Protein Information For CD97 (Source: Uniprot.org, NCBI)
Gene Name
CD97
Full Name
CD97 antigen
Weight
45333 MW
Superfamily
G-protein coupled receptor 2 family
Alternative Names
Golgi membrane protein 1;Golgi membrane protein GP73;Golgi phosphoprotein 2;GOLM1;C9orf155, GOLPH2;PSEC0242, UNQ686/PRO1326;
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CD97, check out the CD97 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CD97: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CD97 Monoclonal Antibody (M02982)
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No publications found for M02982
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Customer Reviews
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Customer Q&As
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5 Customer Q&As for Anti-CD97 Monoclonal Antibody
Question
Would M02982 anti-CD97 Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-03-26
Answer
It shows on the product datasheet, M02982 anti-CD97 Monoclonal antibody as been tested on IF. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-03-26
Question
We are currently using anti-CD97 Monoclonal antibody M02982 for mouse tissue, and we are well pleased with the IF results. The species of reactivity given in the datasheet says human, mouse. Is it likely that the antibody can work on primate tissues as well?
Verified Customer
Verified customer
Asked: 2020-02-21
Answer
The anti-CD97 Monoclonal antibody (M02982) has not been tested for cross reactivity specifically with primate tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-02-21
Question
you antibody to test anti-CD97 Monoclonal antibody M02982 on mouse liver for research purposes, then I may be interested in using anti-CD97 Monoclonal antibody M02982 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-11-28
Answer
The products we sell, including anti-CD97 Monoclonal antibody M02982, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-11-28
Question
Will anti-CD97 Monoclonal antibody M02982 work for IF with liver?
Verified Customer
Verified customer
Asked: 2019-06-27
Answer
According to the expression profile of liver, CD97 is highly expressed in liver. So, it is likely that anti-CD97 Monoclonal antibody M02982 will work for IF with liver.
Boster Scientific Support
Answered: 2019-06-27
Question
Is a blocking peptide available for product anti-CD97 Monoclonal antibody (M02982)?
Verified Customer
Verified customer
Asked: 2019-06-05
Answer
We do provide the blocking peptide for product anti-CD97 Monoclonal antibody (M02982). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-06-05
