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Product Info Summary
| SKU: | M01399-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Rat |
| Host: | Mouse |
| Application: | IHC, WB |
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Product info
Product Name
Anti-CD79b Antibody Picoband®(monoclonal, 6H11)
SKU/Catalog Number
M01399-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-CD79b Antibody Picoband® (monoclonal, 6H11) catalog # M01399-1. Tested in IHC, WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CD79b Antibody Picoband®(monoclonal, 6H11) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01399-1)
Host
Mouse
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Monoclonal
Clone Number
6H11
Isotype
Mouse IgG1
Immunogen
E.coli-derived human CD79b recombinant protein (Position: A29-E229). Human CD79b shares 70% amino acid (aa) sequence identity with mouse CD79b.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
M01399-1 is reactive to CD79B in Human, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500μg/ml.
Observed Molecular Weight
35 kDa
Calculated molecular weight
26.048kDa
Background of CD79B
CD79b molecule, immunoglobulin-associated beta, also known as CD79B (Cluster of Differentiation 79B), is a human gene. By fluorescence in situ hybridization, It is mapped to 17q23.3. The CD79B protein together with the related CD79A protein, forms a dimer associated with membrane bound immunoglobulin in B-cells, thus forming the B-cell antigen receptor (BCR) which is a multimeric complex that includes the antigen-specific component, surface immunoglobulin (Ig). CD79b also can enhances phosphorylation of CD79A, possibly by recruiting kinases which phosphorylate CD79A or by recruiting proteins which bind to CD79A and protect it from dephosphorylation.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01399-1 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat
Positive Control
WB: human Raji whole cell,, human Raji whole cell
IHC: human tonsil tissue, rat spleen tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of CD79b using anti-CD79b antibody (M01399-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human Raji whole cell lysates;
Lane 2: human Raji whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-CD79b antigen affinity purified monoclonal antibody (Catalog # M01399-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for CD79b at approximately 35KD. The expected band size for CD79b is at 26KD.
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Figure 2. IHC analysis of CD79b using anti-CD79b antibody (M01399-1).
CD79b was detected in paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-CD79b Antibody (M01399-1) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.
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Figure 3. IHC analysis of CD79b using anti-CD79b antibody (M01399-1).
CD79b was detected in paraffin-embedded section of rat spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-CD79b Antibody (M01399-1) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For CD79B (Source: Uniprot.org, NCBI)
Gene Name
CD79B
Full Name
B-cell antigen receptor complex-associated protein beta chain
Weight
26.048kDa
Alternative Names
B-cell antigen receptor complex-associated protein beta chain; B-cell-specific glycoprotein B29; Ig-beta; Immunoglobulin-associated B29 protein; CD79b; CD79B; B29, IGB CD79B AGM6, B29, IGB CD79b molecule B-cell receptor complex-associated protein beta chain|B-cell-specific glycoprotein B29|CD79b (immunoglobulin-associated beta)|CD79b molecule, immunoglobulin-associated beta|Ig-beta|immunoglobulin-associated B29 protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CD79B, check out the CD79B Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CD79B: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CD79b Antibody Picoband®(monoclonal, 6H11) (M01399-1)
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