Key=store_id
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Key=sku, value=EK0404
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Key=created_at, value=2014-07-20 01:17:40
Key=updated_at, value=2024-09-26 00:43:52
Key=mst_search_weight, value=0
Key=status, value=Enabled
Key=name, value=Human IL-4/Interleukin-4 ELISA Kit PicoKine®
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Key=template, value=elisa-kits
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Key=price, value=499.0000
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Key=meta_title, value=Human IL-4/Interleukin-4 ELISA Kit Price PicoKine® | BosterBio
Key=meta_description, value=Human IL-4/Interleukin-4 ELISA Kit PicoKine® (96 Tests). Quantitate Human IL4 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).. Sensitivity: 1.5pg/ml.
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Key=url_key, value=human-il-4-picokine-trade-elisa-kit-ek0404-boster
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Key=image_label, value=Human IL-4 PicoKine ELISA Kit standard curve
Key=small_image_label, value=Human IL-4 PicoKine ELISA Kit standard curve
Key=thumbnail_label, value=Human IL-4 PicoKine ELISA Kit standard curve
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Key=size, value=96 wells/kit, with removable strips.
Key=protein_name, value=Interleukin-4
Key=gene_full_name, value=Interleukin-4
Key=uniprot_id, value=P05112
Key=assay_range, value=15.6 pg/ml - 1,000 pg/ml
Key=sensitivity, value=<1.5 pg/ml
Key=applications, value=ELISA
Key=reactivity, value=Human
Key=cross_reactivity, value=There is no detectable cross-reactivity with other relevant proteins.
Key=principle, value=monoclonal antibody from mouse|polyclonal antibody from goat
Key=anticoagulant, value=heparin or EDTA
Key=assay_procedure_step_1, value=1,000 pg/ml, 500 pg/ml, 250 pg/ml, 125 pg/ml, 62.5 pg/ml, 31.2 pg/ml, 15.6 pg/ml
Key=tmb_incubation_time, value=15-25min
Key=normal_range_citations, value=24808641
Key=predicted_reactivity
Key=source_company, value=Boster EK0404
Key=molecular_weight, value=17492 MW
Key=related_product_skus, value=M00230,PROTP05112,PROTP51492,PROTP05112-1,PROTP05112-2,PROTP07750,PROTP20096,PROTP05112-3,PROTP05112-4
Key=product_category, value=Picokine ELISA Kits
Key=research_category, value=Adaptive Immunity, Cancer, Cytokines, Immunology, Innate Immunity, Interleukins, Tumor Immunology
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Key=rating_value, value=100
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Key=cj_related_products, value=Allergy:EK0650|EK2111|PROTQ8BG84|A00490-2|A00490-3|M00490
Key=description, value=Human IL-4/Interleukin-4 ELISA Kit PicoKine® (96 Tests). Quantitate Human IL4 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 1.5pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Key=short_description, value=Human IL-4/Interleukin-4 ELISA Kit PicoKine® (96 Tests). Quantitate Human IL4 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 1.5pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Key=meta_keyword, value=Human IL-4 ELISA Kit, IL4/interleukin 4 ELISA, Bosterbio High Sensitivity
Key=background, value=Interleukin-4 (IL-4), also knowns as a B-cell stimulatory factor1 (BSF1), is an immunomodulatory cytokine, which can inhibit the growth of tumour cells.The human cDNA contains a single open reading frame encoding a protein of 153 amino acids, including a putative signal peptide. IL-4 may act as an autocrine growth factor in pancreatic cancer cells and also give rise to the possibility that cancer-derived IL-4 may suppress cancer-directed immunosurveillance in vivo in addition to its growth-promoting effects, thereby facilitating pancreatic tumor growth and metastasis. The mouse and human genes and their protein products show structural and functional similarities. The human IL-4 gene, which occurs as a single copy in the haploid genome, is mapped on chromosome 5. The standard product used in this kit is recombinant human IL-4, consisting of 130 amino acids with the molecular mass of 14KDa.
Key=gene_name, value=IL4
Key=synonyms, value=Interleukin-4 ;IL-4 ;B-cell stimulatory factor 1 ;Lymphocyte stimulatory factor 1 ;IL4 ;hCG_24113 ;
Key=sample_type, value=cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Key=kit_components, value=10 ng/tube
Key=componentsneeded, value=

• Microplate reader in standard size.
• Automated plate washer.
• Incubator.
• Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
• Clean tubes and Eppendorf tubes.
• Washing buffer (neutral PBS or TBS).
• Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2
• Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.

Key=intra_inter_assay_cv, value=

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	35	216	508	33	195	532
Standard deviation	2.03	12.52	21.33	2.04	11.89	29.26
CV (%)	5.8%	5.8%	4.2%	6.2%	6.1%	5.5%

Key=application_details, value=Dilution ratio of 1:1, concentration in serum and plasma is less than the lowest standard, 12.5 pg/ml.
Key=subcellular_localization, value= Secreted .
Key=protein_function, value= Participates in at least several B-cell activation processes as well as of other cell types. It is a costimulator of DNA-synthesis. It induces the expression of class II MHC molecules on resting B-cells. It enhances both secretion and cell surface expression of IgE and IgG1. It also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. .
Key=sample_data, value=

Concentration (pg/ml)	0	15.6	31.2	62.5	125	250	500	1000
O.D.	0.019	0.030	0.062	0.127	0.229	0.539	0.980	1.851

Key=competitor_equivalent_skus, value=
Key=immunogen, value=Expression system for standard: E.coli; Immunogen sequence: H25-S153
Key=storage, value=Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Key=reproducibility, value=

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	35	31	34	34	33	1.5	4.5%
Sample 2	216	213	203	197	207	7.62	3.6%
Sample 3	508	515	448	502	493	26.52	5.3%

*number of samples for each test n=16.
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