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Product Info Summary
| SKU: | M03651-1 |
|---|---|
| Size: | 100ug/vial |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | Flow Cytometry, IF, IHC, WB |
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Product info
Product Name
Anti-CD50 / ICAM-3 Monoclonal Antibody
View all ICAM-3/CD50 Antibodies
SKU/Catalog Number
M03651-1
Size
100ug/vial
Form
Liquid
Description
Boster Bio Anti-CD50 / ICAM-3 Monoclonal Antibody (Catalog # M03651-1). Tested in Flow Cytometry, IF, WB, IHC applications. This antibody reacts with Human.
Storage & Handling
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Cite This Product
Anti-CD50 / ICAM-3 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03651-1)
Host
Mouse
Contents
Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Clonality
Monoclonal
Clone Number
Clone: ICAM3/1019
Isotype
IgG2b, kappa
Immunogen
Recombinant human ICAM3 protein
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
Does not cross-react with primate, avian or amphibian GR.
Reactive Species
M03651-1 is reactive to ICAM3 in Human
Applications
M03651-1 is guaranteed for Flow Cytometry, IF, IHC, WB Boster Guarantee
Observed Molecular Weight
68 kDa
Calculated molecular weight
59541 MW
Background of ICAM-3/CD50
Recognizes an N-glycosylated glycoprotein of 120kDa with intra-chain disulfide bonds, identified as CD50 or ICAM-3. CD50 is the major ligand for LFA-1 (CD11a/CD18) and may have signaling role to increase adhesion. It is expressed on thymocytes and T lymphocytes and is resistant to treatment with phosphatidylinositol (PI) phospholipase C. This monoclonal antibody is excellent for staining of formalin/paraffin tissues.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Reconstitution
Reconstitute with distilled water.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.
Validation Images & Assay Conditions
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Western Blot analysis of HL-60 cell lysate using Anti-CD50 Monoclonal Antibody (ICAM3/1019)
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Formalin-fixed, paraffin-embedded human Tonsil stained with Anti-CD50 Monoclonal Antibody (ICAM3/1019)
Protein Target Info & Infographic
Gene/Protein Information For ICAM3 (Source: Uniprot.org, NCBI)
Gene Name
ICAM3
Full Name
Intercellular adhesion molecule 3
Weight
59541 MW
Superfamily
immunoglobulin superfamily
Alternative Names
CD50 antigen; CD50; CDW50CDw50; ICAM3; ICAM-3; ICAM-Rintercellular adhesion molecule-3; intercellular adhesion molecule 3 ICAM3 CD50, CDW50, ICAM-R intercellular adhesion molecule 3 intercellular adhesion molecule 3|ICAM-3
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ICAM3, check out the ICAM3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ICAM3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CD50 / ICAM-3 Monoclonal Antibody (M03651-1)
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Customer Q&As
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4 Customer Q&As for Anti-CD50 / ICAM-3 Monoclonal Antibody
Question
Is this M03651-1 anti-CD50 / ICAM-3 Monoclonal antibody reactive to the isotypes of ICAM3?
Verified Customer
Verified customer
Asked: 2020-05-04
Answer
The immunogen of M03651-1 anti-CD50 / ICAM-3 Monoclonal antibody is Recombinant human ICAM3 protein. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-05-04
Question
My question regarding product M03651-1, anti-CD50 / ICAM-3 Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-03-25
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M03651-1 anti-CD50 / ICAM-3 Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-03-25
Question
We are currently using anti-CD50 / ICAM-3 Monoclonal antibody M03651-1 for human tissue, and we are satisfied with the IHC results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on primate tissues as well?
Verified Customer
Verified customer
Asked: 2020-02-27
Answer
The anti-CD50 / ICAM-3 Monoclonal antibody (M03651-1) has not been validated for cross reactivity specifically with primate tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-02-27
Question
Is there a BSA free version of anti-CD50 / ICAM-3 Monoclonal antibody M03651-1 available?
Verified Customer
Verified customer
Asked: 2018-06-29
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-CD50 / ICAM-3 Monoclonal antibody M03651-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-06-29
