Anti-CD50 / ICAM-3 Monoclonal Antibody

Product Name

Anti-CD50 / ICAM-3 Monoclonal Antibody

View all ICAM-3/CD50 Antibodies

SKU/Catalog Number

M03651-1

Size

100ug/vial

Form

Liquid

Description

Boster Bio Anti-CD50 / ICAM-3 Monoclonal Antibody (Catalog # M03651-1). Tested in Flow Cytometry, IF, WB, IHC applications. This antibody reacts with Human.

Storage & Handling

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Cite This Product

Anti-CD50 / ICAM-3 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03651-1)

Host

Mouse

Contents

Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Clonality

Monoclonal

Clone Number

Clone: ICAM3/1019

Isotype

IgG2b, kappa

Immunogen

Recombinant human ICAM3 protein

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Does not cross-react with primate, avian or amphibian GR.

Reactive Species

M03651-1 is reactive to ICAM3 in Human

Reconstitution

Reconstitute with distilled water.

Observed Molecular Weight

68 kDa

Calculated molecular weight

59541 MW

Background of ICAM-3/CD50

Recognizes an N-glycosylated glycoprotein of 120kDa with intra-chain disulfide bonds, identified as CD50 or ICAM-3. CD50 is the major ligand for LFA-1 (CD11a/CD18) and may have signaling role to increase adhesion. It is expressed on thymocytes and T lymphocytes and is resistant to treatment with phosphatidylinositol (PI) phospholipase C. This monoclonal antibody is excellent for staining of formalin/paraffin tissues.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

M03651-1 is guaranteed for Flow Cytometry, IF, IHC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Validation Images & Assay Conditions

Gene/Protein Information For ICAM3 (Source: Uniprot.org, NCBI)

Alternative Names

Intercellular adhesion molecule 3;ICAM-3;CDw50;ICAM-R;CD50;ICAM3; ICAM3 CD50, CDW50, ICAM-R intercellular adhesion molecule 3 intercellular adhesion molecule 3|ICAM-3

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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