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Product Info Summary
| SKU: | PB9555 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-Parvin alpha/PARVA Antibody Picoband®
View all Alpha-parvin/Actopaxin Antibodies
SKU/Catalog Number
PB9555
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Parvin alpha/PARVA Antibody Picoband® catalog # PB9555. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Parvin alpha/PARVA Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9555)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human Parvin alpha, identical to the related mouse sequence, and different from the related rat sequence by one amino acid.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9555 is reactive to PARVA in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
42 kDa
Calculated molecular weight
42244 MW
Background of Alpha-parvin/Actopaxin
Parvin alpha is a protein that in humans is encoded by the PARVA gene. It is located on 11p15.3. PARVA belongs to the parvin family of actin-binding proteins. Parvins are associated with focal contacts and contain calponin homology domains that bind to actin filaments. The encoded protein is part of the integrin-linked kinase signaling complex and plays a role in cell adhesion, motility and survival.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9555 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Positive Control
WB: Rat Liver Tissue, HELA Whole Cell, SW620 Whole Cell, HEPA Whole Cell
IHC: human mammary cancer tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of Parvin alpha using anti-Parvin alpha antibody (PB9555).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.
Lane 1: Rat Liver Tissue Lysate at 50ug,
Lane 2: HELA Whole Cell Lysate at 40ug,
Lane 3: SW620 Whole Cell Lysate at 40ug,
Lane 4: HEPA Whole Cell Lysate at 40ug.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Parvin alpha antigen affinity purified polyclonal antibody (Catalog # PB9555) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Parvin alpha at approximately 42 kDa. The expected band size for Parvin alpha is at 42 kDa.
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Figure 2. IHC analysis of Parvin alpha using anti-Parvin alpha antibody (PB9555).
Parvin alpha was detected in a paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-Parvin alpha Antibody (PB9555) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For PARVA (Source: Uniprot.org, NCBI)
Gene Name
PARVA
Full Name
Alpha-parvin
Weight
42244 MW
Superfamily
parvin family
Alternative Names
Alpha-parvin;Actopaxin;CH-ILKBP;Calponin-like integrin-linked kinase-binding protein;Matrix-remodeling-associated protein 2;PARVA;MXRA2; PARVA CH-ILKBP, MXRA2 parvin alpha alpha-parvin|actopaxin|calponin-like integrin-linked kinase-binding protein|matrix-remodeling-associated protein 2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on PARVA, check out the PARVA Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for PARVA: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Parvin alpha/PARVA Antibody Picoband® (PB9555)
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Customer Q&As
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1 Customer Q&As for Anti-Parvin alpha/PARVA Antibody Picoband®
Question
We are currently using anti-Parvin alpha/PARVA antibody PB9555 for human tissue, and we are content with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on zebrafish tissues as well?
Verified Customer
Verified customer
Asked: 2019-08-07
Answer
The anti-Parvin alpha/PARVA antibody (PB9555) has not been validated for cross reactivity specifically with zebrafish tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-08-07
