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Product Info Summary
| SKU: | M00052-2 |
|---|---|
| Size: | 100uL |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-CD44 Rabbit Monoclonal Antibody, Clone#RM264
SKU/Catalog Number
M00052-2
Size
100uL
Form
Liquid
Description
Boster Bio Anti-CD44 Rabbit Monoclonal Antibody, Clone#RM264 (Catalog # M00052-2). Tested in IHC, WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CD44 Rabbit Monoclonal Antibody, Clone#RM264 (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00052-2)
Host
Rabbit
Contents
50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Clonality
Monoclonal
Clone Number
RM264
Isotype
Rabbit IgG
Immunogen
A peptide corresponding to the extracellular domain of human CD44.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
This antibody reacts to the extracellular domain of human CD44.
Reactive Species
M00052-2 is reactive to CD44 in Human
Reconstitution
Calculated molecular weight
81538 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00052-2 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (IHC): 1:1000-1:5000 dilution
WB: 1:1000-1:2000 dilution.
Validation Images & Assay Conditions
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Figure 1. Western Blotting result
Western Blot of HeLa cells lysates using Anti-CD44 Rabbit Monoclonal Antibody (Clone RM264) at a 1:1000 dilution.
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Figure 2. IHC result
Immunohistochemical staining of formalin fixed and paraffin embedded human breast cancer tissue sections using Anti-CD44 Rabbit Monoclonal Antibody (Clone RM264) at a 1:5000 dilution.
Protein Target Info & Infographic
Gene/Protein Information For CD44 (Source: Uniprot.org, NCBI)
Gene Name
CD44
Full Name
CD44 antigen
Weight
81538 MW
Alternative Names
CD44 antigen;CDw44;Epican;Extracellular matrix receptor III;ECMR-III;GP90 lymphocyte homing/adhesion receptor;HUTCH-I;Heparan sulfate proteoglycan;Hermes antigen;Hyaluronate receptor;Phagocytic glycoprotein 1;PGP-1;Phagocytic glycoprotein I;PGP-I;CD44;CD44;LHR, MDU2, MDU3, MIC4; CD44 CDW44, CSPG8, ECMR-III, HCELL, HUTCH-I, IN, LHR, MC56, MDU2, MDU3, MIC4, Pgp1 CD44 molecule (Indian blood group) CD44 |GP90 lymphocyte homing/adhesion receptor|Hermes |Indian blood group |cell surface glycoprotein CD44|chondroitin sulfate proteoglycan 8|epican|extracellular matrix receptor III|hematopoietic cell E- and L-selectin ligand|heparan sulfate proteoglycan|homing function and Indian blood group system|hyaluronate receptor|phagocytic glycoprotein 1|soluble CD44
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CD44, check out the CD44 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CD44: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CD44 Rabbit Monoclonal Antibody, Clone#RM264 (M00052-2)
Hello CJ!
M00052-2 has been cited in 4 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Zhang GF,Wu JC,Wang HY,Jiang WD,Qiu L. Overexpression of microRNA-205-5p exerts suppressive effects on stem cell drug resistance in gallbladder cancer by down-regulating PRKCE. Biosci Rep.2020 Sep 30;40(9): BSR20194509.doi:10.1042/BSR20194509.PMID: 328698
Species: Human,Mouse
M00052-2 usage in article: APP:FLOW CYTOMETRY, SAMPLE:GBC STEM CELLS, DILUTION:NA
Kubatka P,Kello M,Kajo K,Samec M,Liskova A,Jasek K,Koklesova L,Kuruc T,Adamkov M,Smejkal K,Svajdlenka E,Solar P,Pec M,Büsselberg D,Sadlonova V,Mojzis J.Rhus coriaria L.(Sumac) Demonstrates Oncostatic Activity in the Therapeutic and Preventive Model of Bre
Species: Human,Mouse,Rat
M00052-2 usage in article: APP:IHC, SAMPLE:MAMMARY TISSUE, DILUTION:NA
Antineoplastic effects of clove buds (Syzygium aromaticum L.) in the model of breast carcinoma
Rat nasal respiratory mucosa-derived ectomesenchymal stem cells differentiate into Schwann-like cells promoting the differentiation of PC12 cells and %u2026
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Customer Reviews
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Customer Q&As
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16 Customer Q&As for Anti-CD44 Rabbit Monoclonal Antibody, Clone#RM264
Question
Does M00052-2 anti-CD44 Rabbit Monoclonal antibody, Clone#RM264 work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-03-04
Answer
You can see on the product datasheet, M00052-2 anti-CD44 Rabbit Monoclonal antibody, Clone#RM264 as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-03-04
Question
We were satisfied with the WB result of your anti-CD44 Rabbit Monoclonal antibody, Clone
A. Zhang
Verified customer
Asked: 2020-02-24
Answer
Based on literature, mammary carcinoma does express CD44. Generally CD44 expresses in cell membrane. Regarding which tissues have CD44 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 17081983, 18669648, 18691976, 20068231, 23186163
Foreskin, Pubmed ID: 2007624
Glial tumor, Pubmed ID: 7527301
Keratinocyte, Pubmed ID: 1281868
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 19159218, 24275569
Lung, Pubmed ID: 1717145, 7528188
Lymphoblast, Pubmed ID: 1465456, 1922057
Mammary carcinoma, Pubmed ID: 8352881
Myeloid leukemia cell, Pubmed ID: 2056274
Pancreas, and Retinal pigment epithelium, Pubmed ID: 15489334
Peripheral blood, Pubmed ID: 7508992
Plasma, Pubmed ID: 16335952
Reticulocyte, Pubmed ID: 1840487
Spinal cord, Pubmed ID: 17974005
T-cell, Pubmed ID: 19367720
Boster Scientific Support
Answered: 2020-02-24
Question
We ordered your anti-CD44 Rabbit Monoclonal antibody, Clone
Verified Customer
Verified customer
Asked: 2020-02-03
Answer
I have checked the website and datasheets of our anti-CD44 Rabbit Monoclonal antibody, Clone
Boster Scientific Support
Answered: 2020-02-03
Question
Please see the WB image, lot number and protocol we used for plasma using anti-CD44 Rabbit Monoclonal antibody, Clone#RM264 M00052-2. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-12-23
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-12-23
Question
Is a blocking peptide available for product anti-CD44 Rabbit Monoclonal antibody, Clone#RM264 (M00052-2)?
Verified Customer
Verified customer
Asked: 2019-09-17
Answer
We do provide the blocking peptide for product anti-CD44 Rabbit Monoclonal antibody, Clone#RM264 (M00052-2). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-09-17
Question
I was wanting to use your anti-CD44 Rabbit Monoclonal antibody, Clone#RM264 for WB for human plasma on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human plasma identification?
Verified Customer
Verified customer
Asked: 2019-09-11
Answer
You can see on the product datasheet, M00052-2 anti-CD44 Rabbit Monoclonal antibody, Clone#RM264 has been validated for IHC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human plasma in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-09-11
Question
I see that the anti-CD44 Rabbit Monoclonal antibody, Clone#RM264 M00052-2 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-08-16
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-08-16
Question
Is this M00052-2 anti-CD44 Rabbit Monoclonal antibody, Clone#RM264 reactive to the isotypes of CD44?
Verified Customer
Verified customer
Asked: 2019-05-31
Answer
The immunogen of M00052-2 anti-CD44 Rabbit Monoclonal antibody, Clone#RM264 is A peptide corresponding to the extracellular domain of human CD44. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-05-31
Question
We need using your anti-CD44 Rabbit Monoclonal antibody, Clone#RM264 for hyaluronan catabolic process studies. Has this antibody been tested with western blotting on breast cancer tissue? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2018-12-03
Answer
Thanks for your inquiry. This M00052-2 anti-CD44 Rabbit Monoclonal antibody, Clone#RM264 is validated on hela cells, breast cancer tissue. It is guaranteed to work for IHC, WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2018-12-03
Question
We have seen staining in human keratinocyte. Do you have any suggestions? Is anti-CD44 Rabbit Monoclonal antibody, Clone#RM264 supposed to stain keratinocyte positively?
Verified Customer
Verified customer
Asked: 2018-09-12
Answer
From what I have seen in literature keratinocyte does express CD44. From what I have seen in Uniprot.org, CD44 is expressed in parotid gland, reticulocyte, myeloid leukemia cell, keratinocyte, lymphoblast, mammary carcinoma, articular cartilage, colon adenocarcinoma retinal pigment epithelium, spinal cord, pancreas retinal pigment epithelium, glial tumor, peripheral blood, foreskin, lung, plasma, cervix carcinoma, t-cell, liver, leukemic t-cell, among other tissues. Regarding which tissues have CD44 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 17081983, 18669648, 18691976, 20068231, 23186163
Foreskin, Pubmed ID: 2007624
Glial tumor, Pubmed ID: 7527301
Keratinocyte, Pubmed ID: 1281868
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 19159218, 24275569
Lung, Pubmed ID: 1717145, 7528188
Lymphoblast, Pubmed ID: 1465456, 1922057
Mammary carcinoma, Pubmed ID: 8352881
Myeloid leukemia cell, Pubmed ID: 2056274
Pancreas, and Retinal pigment epithelium, Pubmed ID: 15489334
Peripheral blood, Pubmed ID: 7508992
Plasma, Pubmed ID: 16335952
Reticulocyte, Pubmed ID: 1840487
Spinal cord, Pubmed ID: 17974005
T-cell, Pubmed ID: 19367720
Boster Scientific Support
Answered: 2018-09-12
Question
Is there a BSA free version of anti-CD44 Rabbit Monoclonal antibody, Clone#RM264 M00052-2 available?
G. Gonzalez
Verified customer
Asked: 2017-10-10
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-CD44 Rabbit Monoclonal antibody, Clone#RM264 M00052-2 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2017-10-10
Question
Does anti-CD44 Rabbit Monoclonal antibody, Clone#RM264 M00052-2 work for WB with plasma?
K. Krishna
Verified customer
Asked: 2017-02-08
Answer
According to the expression profile of plasma, CD44 is highly expressed in plasma. So, it is likely that anti-CD44 Rabbit Monoclonal antibody, Clone#RM264 M00052-2 will work for WB with plasma.
Boster Scientific Support
Answered: 2017-02-08
Question
My question regarding product M00052-2, anti-CD44 Rabbit Monoclonal antibody, Clone#RM264. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
P. Li
Verified customer
Asked: 2016-03-03
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00052-2 anti-CD44 Rabbit Monoclonal antibody, Clone#RM264, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2016-03-03
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for plasma using anti-CD44 Rabbit Monoclonal antibody, Clone#RM264 M00052-2. Let me know if you need anything else.
C. Dhar
Verified customer
Asked: 2014-12-22
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2014-12-22
Question
We need to test anti-CD44 Rabbit Monoclonal antibody, Clone#RM264 M00052-2 on human plasma for research purposes, then I may be interested in using anti-CD44 Rabbit Monoclonal antibody, Clone#RM264 M00052-2 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
A. Jones
Verified customer
Asked: 2014-01-03
Answer
The products we sell, including anti-CD44 Rabbit Monoclonal antibody, Clone#RM264 M00052-2, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2014-01-03
Question
We are currently using anti-CD44 Rabbit Monoclonal antibody, Clone#RM264 M00052-2 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on bovine tissues as well?
P. Lewis
Verified customer
Asked: 2013-03-14
Answer
The anti-CD44 Rabbit Monoclonal antibody, Clone#RM264 (M00052-2) has not been tested for cross reactivity specifically with bovine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2013-03-14
