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Product Info Summary
| SKU: | RP1105 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Mouse |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-CD33 Antibody Picoband®
View all Siglec-3/CD33 Antibodies
SKU/Catalog Number
RP1105
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-CD33 Antibody catalog # RP1105. Tested in IHC, WB applications. This antibody reacts with Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CD33 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # RP1105)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2mg Na2HPO4, 0.01 mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived mouse CD33 recombinant protein (Position: D18-E240). Mouse CD33 shares 61.6% amino acid (aa) sequence identity with human CD33.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
RP1105 is reactive to Cd33 in Mouse
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
26 kDa
Calculated molecular weight
44824 MW
Background of Siglec-3/CD33
CD33, also known as Siglec-3 (sialic acid binding Ig-like lectin 3, SIGLEC3, SIGLEC-3, gp67, p67), is a transmembrane receptor expressed on cells of myeloid lineage. It is usually considered myeloid-specific, but it can also be found on some lymphoid cells. CD33 binds sialic acids, therefore is a member of the SIGLEC family of lectins. By fluorescence in situ hybridization, CD33 is mapped to 19q13.3-q13.4.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
RP1105 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Mouse, By Heat
Western blot, 0.1-0.5μg/ml, Mouse
Positive Control
WB: mosue lung extract (mouse spleen tissue
Validation Images & Assay Conditions
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CD33 was detected in paraffin-embedded sections of mouse spleen tissues using rabbit anti-CD33 Antigen Affinity purified polyclonal antibody (Catalog # RP1105) at 1 μg/mL. The immunohistochemical section was developed using SABC method (Catalog # SA1022).
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Western blot analysis of CD33 expression in mosue lung extract (lane 1). CD33 at 45KD was detected using rabbit anti-CD33 Antigen Affinity purified polyclonal antibody (Catalog # RP1105) at 0.5 μg/mL. The blot was developed using chemiluminescence (ECL) method (Catalog # EK1002).
Protein Target Info & Infographic
Gene/Protein Information For Cd33 (Source: Uniprot.org, NCBI)
Gene Name
Cd33
Full Name
Myeloid cell surface antigen CD33
Weight
44824 MW
Superfamily
immunoglobulin superfamily
Alternative Names
Myeloid cell surface antigen CD33;Sialic acid-binding Ig-like lectin 3;Siglec-3;CD33;Cd33;Siglec3; CD33 SIGLEC-3, SIGLEC3, p67 CD33 molecule myeloid cell surface CD33|CD33 (gp67)|CD33 molecule transcript|gp67|sialic acid-binding Ig-like lectin 3
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Cd33, check out the Cd33 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Cd33: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CD33 Antibody Picoband® (RP1105)
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No publications found for RP1105
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Customer Reviews
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Customer Q&As
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15 Customer Q&As for Anti-CD33 Antibody Picoband®
Question
I see that the anti-CD33 antibody RP1105 works with IHC, what is the protocol used to produce the result images on the product page?
B. Edwards
Verified customer
Asked: 2020-03-30
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2020-03-30
Question
I was wanting to use to test anti-CD33 antibody RP1105 on mouse premonocytic lymphoma for research purposes, then I may be interested in using anti-CD33 antibody RP1105 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-02-21
Answer
The products we sell, including anti-CD33 antibody RP1105, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-02-21
Question
Please see the WB image, lot number and protocol we used for premonocytic lymphoma using anti-CD33 antibody RP1105. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-11-29
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-11-29
Question
We have tried in the past anti-CD33 antibody for IHC on leukocyte in a previous experiment. I am using mouse, and We are going to use the antibody for WB next. I am looking for examining leukocyte as well as premonocytic lymphoma in our next experiment. Could you please give me some suggestion on which antibody would work the best for WB?
Verified Customer
Verified customer
Asked: 2019-11-26
Answer
I took a look at the website and datasheets of our anti-CD33 antibody and it seems that RP1105 has been validated on mouse in both IHC and WB. Thus RP1105 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-11-26
Question
Will anti-CD33 antibody RP1105 work for IHC with premonocytic lymphoma?
Verified Customer
Verified customer
Asked: 2019-10-29
Answer
According to the expression profile of premonocytic lymphoma, CD33 is highly expressed in premonocytic lymphoma. So, it is likely that anti-CD33 antibody RP1105 will work for IHC with premonocytic lymphoma.
Boster Scientific Support
Answered: 2019-10-29
Question
Is a blocking peptide available for product anti-CD33 antibody (RP1105)?
Verified Customer
Verified customer
Asked: 2019-07-19
Answer
We do provide the blocking peptide for product anti-CD33 antibody (RP1105). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-07-19
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for premonocytic lymphoma using anti-CD33 antibody RP1105. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-05-13
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-05-13
Question
My colleagues were satisfied with the WB result of your anti-CD33 antibody. However we have been able to see positive staining in leukocyte isoform cd33m: cell membrane using this antibody. Is that expected? Could you tell me where is CD33 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-04-22
Answer
From literature, leukocyte does express CD33. Generally CD33 expresses in isoform cd33m: cell membrane, isoform cd33m: peroxisome. Regarding which tissues have CD33 expression, here are a few articles citing expression in various tissues:
Leukocyte, Pubmed ID: 15489334
Premonocytic lymphoma, Pubmed ID: 3139766, 11943481
Uterus, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2019-04-22
Question
My question regarding product RP1105, anti-CD33 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2017-10-26
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free RP1105 anti-CD33 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-10-26
Question
Is this RP1105 anti-CD33 antibody reactive to the isotypes of CD33?
C. Anderson
Verified customer
Asked: 2016-12-08
Answer
The immunogen of RP1105 anti-CD33 antibody is E. coli-derived mouse CD33 recombinant protein (Position: D18-E240). Mouse CD33 shares 61.6% amino acid (aa) sequence identity with human CD33. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2016-12-08
Question
Would RP1105 anti-CD33 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
J. Kulkarni
Verified customer
Asked: 2016-09-14
Answer
It shows on the product datasheet, RP1105 anti-CD33 antibody as been tested on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2016-09-14
Question
We are currently using anti-CD33 antibody RP1105 for mouse tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says mouse. Is it likely that the antibody can work on zebrafish tissues as well?
G. Zhang
Verified customer
Asked: 2016-04-25
Answer
The anti-CD33 antibody (RP1105) has not been validated for cross reactivity specifically with zebrafish tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2016-04-25
Question
We have seen staining in mouse uterus. What should we do? Is anti-CD33 antibody supposed to stain uterus positively?
G. Jackson
Verified customer
Asked: 2015-10-15
Answer
From literature uterus does express CD33. From Uniprot.org, CD33 is expressed in leukocyte, premonocytic lymphoma, uterus, among other tissues. Regarding which tissues have CD33 expression, here are a few articles citing expression in various tissues:
Leukocyte, Pubmed ID: 15489334
Premonocytic lymphoma, Pubmed ID: 3139766, 11943481
Uterus, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2015-10-15
Question
Do you have a BSA free version of anti-CD33 antibody RP1105 available?
P. Li
Verified customer
Asked: 2015-05-05
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-CD33 antibody RP1105 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2015-05-05
Question
I was wanting to use your anti-CD33 antibody for IHC for mouse premonocytic lymphoma on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse premonocytic lymphoma identification?
K. Miller
Verified customer
Asked: 2013-04-09
Answer
It shows on the product datasheet, RP1105 anti-CD33 antibody has been validated for IHC, WB on mouse tissues. We have an innovator award program that if you test this antibody and show it works in mouse premonocytic lymphoma in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2013-04-09
