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Product Info Summary
| SKU: | M01450-1 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | IP, IF, IHC, WB |
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Product info
Product Name
Anti-CD32A (FCGR2A) Mouse Monoclonal Antibody [Clone ID: OTI13D7]
SKU/Catalog Number
M01450-1
Size
100 µl
Description
Boster Bio FCGR2A (CD32) mouse monoclonal antibody, clone OTI13D7 (formerly 13D7). Catalog# M01450-1. Tested in IF, IHC, IP, WB. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CD32A (FCGR2A) Mouse Monoclonal Antibody [Clone ID: OTI13D7] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01450-1)
Host
Mouse
Contents
PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Clonality
Monoclonal
Clone Number
OTI13D7
Isotype
IgG2b
Immunogen
Full length human recombinant protein of human FCGR2A (NP_067674) produced in HEK293T cell.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M01450-1 is reactive to FCGR2A in Human
Reconstitution
Calculated molecular weight
35.001kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01450-1 is guaranteed for IP, IF, IHC, WB Boster Guarantee
Validation Images & Assay Conditions
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Anti-FCGR2A mouse monoclonal antibody (M01450-1) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY FCGR2A.
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Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-FCGR2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-FCGR2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-FCGR2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-FCGR2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-FCGR2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-FCGR2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-FCGR2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Human thyroid tissue within the normal limits using anti-FCGR2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-FCGR2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-FCGR2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FCGR2A (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FCGR2A.
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Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-FCGR2A monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human) (1:200).
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Western blot analysis of extracts (10ug) from 1 cell line by using anti-FCGR2A monoclonal antibody at 1:200.
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Western blot analysis of extracts (35ug) from 1 cell line lysate by using anti-FCGR2A monoclonal antibody (1:500).
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Western blot analysis of extracts (35ug) from 6 tissue lysates by using anti-FCGR2A monoclonal antibody (1:500).
Protein Target Info & Infographic
Gene/Protein Information For FCGR2A (Source: Uniprot.org, NCBI)
Gene Name
FCGR2A
Full Name
Low affinity immunoglobulin gamma Fc region receptor II-a
Weight
35.001kDa
Alternative Names
CD32 antigen; CD32a; CD32MGC23887; CDw32fc-gamma-RIIa; Fc fragment of IgG, low affinity IIa, receptor (CD32); Fc fragment of IgG, low affinity IIa, receptor for (CD32); Fc gamma RIIA; FCG2; Fc-gamma RII-a; Fc-gamma-RIIa; FcGR; FCGR2; FCGR2A; FCGR2A1; FcgRIIA; FCRIIA; fcRII-a; IGFR2MGC30032; IgG Fc receptor II-a; Immunoglobulin G Fc receptor II; low affinity immunoglobulin gamma Fc region receptor II-a FCGR2A CD32, CD32A, CDw32, FCG2, FCGR21, FcGR, IGFR2, FCGR2A Fc fragment of IgG receptor IIa low affinity immunoglobulin gamma Fc region receptor II-a|Fc fragment of IgG, low affinity IIa, receptor (CD32)|Fc gamma receptor RIIa3|Immunoglobulin G Fc receptor II|fc-gamma-RIIa|fcRII-a|igG Fc receptor II-a
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on FCGR2A, check out the FCGR2A Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for FCGR2A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CD32A (FCGR2A) Mouse Monoclonal Antibody [Clone ID: OTI13D7] (M01450-1)
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