Product Info Summary
SKU: | M04237 |
---|---|
Size: | 100 μl |
Reactive Species: | Human |
Host: | Rabbit |
Application: | Flow Cytometry, IHC, WB |
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Product info
Product Name
Anti-CD23 FCER2 Monoclonal Antibody
View all CD23/Fc epsilon RII Antibodies
SKU/Catalog Number
M04237
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-CD23 FCER2 Monoclonal Antibody catalog # M04237. Tested in WB, IHC, Flow Cytometry applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CD23 FCER2 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M04237)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
ADIC-6
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human CD23 This receptor has essential roles in the regulation of IgE production and in the differentiation of B-cells (it is a B-cell-specific antigen) .
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M04237 is reactive to FCER2 in Human
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
48 kDa
Calculated molecular weight
366649 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M04237 is guaranteed for Flow Cytometry, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:1000
IHC 1:50-1:200
FC 1:30
Positive Control
WB: Raji cell
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of CD23 expression in Raji cell lysate.
Protein Target Info & Infographic
Gene/Protein Information For FCER2 (Source: Uniprot.org, NCBI)
Gene Name
FCER2
Full Name
Low affinity immunoglobulin epsilon Fc receptor
Weight
366649 MW
Alternative Names
Laminin subunit alpha-3;Epiligrin 170 kDa subunit;E170;Epiligrin subunit alpha;Kalinin subunit alpha;Laminin-5 subunit alpha;Laminin-6 subunit alpha;Laminin-7 subunit alpha;Nicein subunit alpha;LAMA3;LAMNA; FCER2 BLAST-2, CD23, CD23A, CLEC4J, FCE2, IGEBF Fc fragment of IgE receptor II low affinity immunoglobulin epsilon Fc receptor|C-type lectin domain family 4, member J|CD23 |Fc epsilon receptor II|Fc fragment of IgE, low affinity II, receptor for (CD23)|fc-epsilon-RII|immunoglobulin E-binding factor|immunoglobulin epsilon-chain|lymphocyte IgE receptor
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on FCER2, check out the FCER2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for FCER2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CD23 FCER2 Monoclonal Antibody (M04237)
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No publications found for M04237
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Customer Q&As
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4 Customer Q&As for Anti-CD23 FCER2 Monoclonal Antibody
Question
See attached the WB image, lot number and protocol we used for b-cell using anti-CD23 Monoclonal antibody M04237. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-10-24
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-10-24
Question
I have a question about product M04237, anti-CD23 Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-05-28
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M04237 anti-CD23 Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-05-28
Question
Is a blocking peptide available for product anti-CD23 Monoclonal antibody (M04237)?
M. Moore
Verified customer
Asked: 2018-05-24
Answer
We do provide the blocking peptide for product anti-CD23 Monoclonal antibody (M04237). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2018-05-24
Question
I am looking for to test anti-CD23 Monoclonal antibody M04237 on human b-cell for research purposes, then I may be interested in using anti-CD23 Monoclonal antibody M04237 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2018-03-22
Answer
The products we sell, including anti-CD23 Monoclonal antibody M04237, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-03-22