FCER2 Antibodies

Low affinity immunoglobulin epsilon Fc receptor (FCER2)

Low-affinity receptor for immunoglobulin E (IgE) and CR2/CD21. Has essential functions in the regulation of IgE production and in the distinction of B-cells (it is a B-cell-specific antigen).

.

Gene Information

Human
Gene Name:	FCER2
Uniprot:	P06734
Entrez:	2208

Family

Belongs to:
No superfamily

Alternative Names

BLAST-2; CD23; CD23A; CD23CD23 antigen; CLEC4J; CLEC4JC-type lectin domain family 4 member J; C-type lectin domain family 4, member J; Fc epsilon RII; Fc fragment of IgE, low affinity II, receptor for (CD23); FCE2Fc fragment of IgE, low affinity II, receptor for (CD23A); fc-epsilon-RII; FCER2; Fcer2a; FceRII; IGEBF; Immunoglobulin E-binding factor; low affinity immunoglobulin epsilon Fc receptor; Ly-42; Lymphocyte IgE receptor

Molecular Weight

Mass (kDA):
36.469 kDA

Genomic Context

Human
Location:	19p13.2
Sequence:	19; NC_000019.10 (7688757..7702755, complement)

Subcellular Localizations

Cell membrane; Single-pass type II membrane protein. Cell membrane; Lipid-anchor. Secreted. Also exists as a soluble excreted form, sCD23.

Diseases

• Asthma
• Neoplasms
• Lymphoma
• Severe Acute Respiratory Syndrome
• Sclerosis
• Inflammatory Bowel Diseases
• Malignant Neoplasm Of Lung
• Inflammatory Response
• Leukemia, Myelocytic, Acute
• Kimura Disease
• Immunologic Deficiency Syndromes
• Variable Number Tandem Repeat
• Malignant Neoplasms

• Severe Combined Immunodeficiency
• Parotid Gland Warthin Tumor
• Chronic Lymphocytic Leukemia
• Celiac Disease
• Bronchial Hyperreactivity
• Hereditary Diseases
• Lung Neoplasms

Interacting Proteins

• ATF7

Pathways

• Immune Response
• Cell Activation
• T Cell Activation
• Pathogenesis
• Inflammatory Response

PTMs

• Disulfide bond
• Glycoprotein

• Lipoprotein
• Palmitate

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/FCER2

Best Uses Of The FCER2 Marker With Boster Bio

Optimizing your experiments with Boster Bio can be a daunting task. Fortunately, a comprehensive Boster Bio optimization guide can provide answers to many of the most common questions, guiding you towards more accurate results. Even though all researchers face trials and errors during experiments, proper controls and troubleshooting can eliminate many of the sources of error. These guides will help you find the sources of error and eliminate them from your experiments.

High-affinity primary antibodies

In order to develop high-affinity primary antibodies that specifically recognize the FCER2 marker, we first cloned a panel of incrementally affinity TCRs. We then used lentiviral transduced 293 T cells to infect primary CD8 T cells. After transduction, cells were stimulated with a mixture of CD3/CD28 beads and PHA/A2neg feeder cells and sorted using a PE-labeled A2/NY-ESO-1157-165 multimer. We then performed flow cytometry to analyze these cells and identify their phenotypic properties.

These antibody molecules recognize the FCER2 receptor via binding to the sCD23 integrin. These antibodies have been developed to mimic the cytokine-like properties of CD23 by inhibiting its binding to monocytes. The affinity-based approaches are also useful in establishing the funlls. of the FCER2 receptor . monocytic cells. High-affinity primary antibodies using the FCER2 marker have been developed in recent years to detect FCER2 in a variety of cancer cells.

These studies have revealed that affinity-increased TCRs are directly tuned to the funlls. al potential of CD8 T cells. Chronic interalls. s between these antibodies and self-A2 molecules can drive T cell allsvation. Therefore, affinity-mediated hyporesponsiveness is a novel and potentially useful strategy for developing high-affinity primary antibodies. These antibodies target and kill the tumor cells by reprogramming the TCRs of neoantigen-redirected CD8 T cells.

A2pos primary T cells have a reduced surface TCR/CD3e. These T cells exhibit high affinity for HLA-A2/NY-ESO-1 tumor antigens. However, they have a significantly reduced surface TCR/CD3e-restricted FCER2 T cells. However, they retain their HLA-A2 phenotype, thereby facilitating the development of effective immunotherapy.

ELISA kits

ELISA kits use sandwich enzyme-linked immuno-sorbent assay technology to detect molecules and proteins. They include a pre-coated plate, capture/detects. antibodies, buffers, standards, and accessory reagents. ELISA kits using the FCER2 marker have been developed to detect up to 300 different proteins and molecules. They are accurate and convenient and can detect targets of interest in samples in a matter of hours. They also include pre-coated plates, capture/detects. antibodies, and buffers for each stage. And because they undergo stability testing, they are guaranteed to work accurately and reliably.

ELISA kits using the FCER2 protein marker are designed to detect this protein by binding to the marker. ELISA plate preparals. s should be made from antigen-tagged antibody that is specific for the marker. Antibody pair kits contain optimized matched antibodies that are specifically designed for sandwich ELISA development. Antibody-coupled ELISA kits use the FCER2 antibody, and are available as uncoated and coated formats.

The FCER2 ELISA kit is designed for the in vitro quantitative measurement of the concentrals. of Mouse Low Affinity Immunoglobulin Epsilon Fc Receptor/CD23 (FCER2) in biological fluids. Its diluls. s are based on the end-user's requirements. ELISA kits using the FCER2 marker should be stored at 4 degC and are valid for 6 months.

ELISA is useful for measuring the concentrals. of this protein in crude samples. Because the kit uses high-affinity antibodies, it allows nonspecific bound material to be washed away. Consequently, ELISA is a useful tool for measuring specific analytes in crude preparals. s. It has several advantages. So, it is recommended to use the FCER2 marker ELISA kit to analyze a variety of biological samples.

FCER2 marker

The FCER2 marker is a member of the Caspase family. The enzymes are allsvated by pro-apoptotic stimuli such as tumor necrosis factor and growth factor receptor-bound proteins. Other pro-apoptotic factors include Granzyme-B, calcium channels, and DNA damage. Boster Bio offers high-affinity, reagent-grade FCER2 antibodies and a variety of other convenient soluls. s for PCR.