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Product Info Summary
| SKU: | EK0924 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Mouse |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). |
Product info
Product Name
Mouse CD23/FCER2 ELISA Kit PicoKine®
View all CD23/Fc epsilon RII ELISA kits
SKU/Catalog Number
EK0924
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Mouse CD23/FCER2 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Fcer2 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 15pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Mouse CD23/FCER2 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0924)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: NS0; Immunogen sequence: E50-P331
Sensitivity
<15 pg/ml
Assay Range
156 pg/ml - 10,000 pg/ml
Standard Dilution Instructions
See datasheet of EK0924 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK0924 is reactive to Fcer2 in Mouse samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Application Guarantee
EK0924 is guaranteed for ELISA in Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of CD23/Fc epsilon RII
CD23, also known as Fc epsilon RII, or FcεRII, is the "low-affinity" receptor for IgE, an antibody isotype involved in allergy and resistance to parasites, and is important in regulation of IgE levels. There are two forms of CD23: CD23a and CD23b. CD23a is present on follicular B cells, whereas CD23b requires IL-4 to be expressed on T-cells, monocytes, Langerhans cells, eosinophils, and macrophages. As part of a mapping of multiple probes to specific bands on chromosome 19 by fluorescence in situ hybridization, the CD23 gene was assigned to 19p13.3. And CD23 (FCE2) is a key molecule for B-cell activation and growth. It is the low-affinity receptor for IgE. The truncated molecule can be secreted, then functioning as a potent mitogenic growth factor.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0924-CAP | Anti-Mouse Fcer2 Pre-coated 96-well strip microplate | 1 |
| EK0924-ST | Mouse Fcer2 Standard | 2 vials, 10 ng/tube |
| EK0924-DA | Mouse Fcer2 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
| O.D. | 0.021 | 0.045 | 0.137 | 0.279 | 0.509 | 1.009 | 1.503 | 2.041 |
Data Example Images
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Mouse CD23/FCER2 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect CD23/Fc epsilon RII, Dilution ratio of 1:1, concentration in serum and plasma is around 400 pg/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Mouse CD23/FCER2 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 256 | 1831 | 5853 | 234 | 1881 | 5493 |
| Standard deviation | 11 | 137.32 | 345.32 | 12.87 | 156.12 | 373.52 |
| CV (%) | 4.3% | 7.5% | 5.9% | 5.5% | 8.3% | 6.8% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of CD23/Fc epsilon RII in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 256 | 296 | 293 | 254 | 274 | 19.79 | 7.2% |
| Sample 2 | 1831 | 1672 | 1609 | 1822 | 1733 | 95.68 | 5.5% |
| Sample 3 | 5853 | 5387 | 5221 | 5268 | 5432 | 250.34 | 4.6% |
Protein Target Info & Infographic
Gene/Protein Information For Fcer2 (Source: Uniprot.Org, NCBI)
Gene Name
Fcer2
Full Name
Low affinity immunoglobulin epsilon Fc receptor
Weight
37648 MW
Alternative Names
Low affinity immunoglobulin epsilon Fc receptor;Fc-epsilon-RII;Lymphocyte IgE receptor;CD23;Fcer2;Fcer2a; FCER2 BLAST-2, CD23, CD23A, CLEC4J, FCE2, IGEBF Fc fragment of IgE receptor II low affinity immunoglobulin epsilon Fc receptor|C-type lectin domain family 4, member J|CD23 |Fc epsilon receptor II|Fc fragment of IgE, low affinity II, receptor for (CD23)|fc-epsilon-RII|immunoglobulin E-binding factor|immunoglobulin epsilon-chain|lymphocyte IgE receptor
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on Fcer2, check out the Fcer2 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for Fcer2: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Mouse CD23/FCER2 ELISA Kit PicoKine® (EK0924)
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Customer Q&As
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7 Customer Q&As for Mouse CD23/FCER2 ELISA Kit PicoKine®
Question
Q: we need your suggestion regarding the dilution ratio of serum samples for detection of CD23 in Mouse cell lysates? I am trying to measure a few parameters and it requires 100ul of diluted samples for each well. We have limited sample volumes so we like to dilute as much as possible.
Verified Customer
Verified customer
Asked: 2020-11-07
Answer
A: having little idea about the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the CD23 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2020-11-07
Question
Q: how to analyze ELISA data? I have obtained CD23 level in cell lysates.
Verified Customer
Verified customer
Asked: 2019-11-19
Answer
A: we recommend you this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2019-11-19
Question
Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for measurement CD23 using the EK0924 Mouse CD23 Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2019-10-02
Answer
A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may change the stabilityof samples. It is recommend to assay sample right after collection when possible, or aliquot into single use volumes and store samples frozen. limit repetitive freeze-thaw cycles with the stored samples to avoid protein degradation.
Boster Scientific Support
Answered: 2019-10-02
Question
Q: how to thaw whole blood sample for CD23 ELISA after freezing?
Verified Customer
Verified customer
Asked: 2019-09-28
Answer
A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test CD23 for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.
Boster Scientific Support
Answered: 2019-09-28
Question
Q: can you tell me how to prepare cell lysates prepared for use in Picokine® ELISA kits?
Verified Customer
Verified customer
Asked: 2019-09-16
Answer
A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are present in the product insert. Components in lysate and lysis buffer may affect immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2019-09-16
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
Verified Customer
Verified customer
Asked: 2019-08-14
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is a must for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2019-08-14
Question
Q: how much samples can be assayed in a Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2017-03-15
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may might differ by kit so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2017-03-15
