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Product Info Summary
| SKU: | M05546-1 |
|---|---|
| Size: | 100uL |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-Terminal deoxynucleotidyl transferase (TdT) DNTT Rabbit Monoclonal Antibody, Clone#RM379
SKU/Catalog Number
M05546-1
Size
100uL
Form
Liquid
Description
Boster Bio Anti-Terminal deoxynucleotidyl transferase (TdT) DNTT Rabbit Monoclonal Antibody, Clone#RM379 (Catalog # M05546-1). Tested in IHC, WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Terminal deoxynucleotidyl transferase (TdT) DNTT Rabbit Monoclonal Antibody, Clone#RM379 (Boster Biological Technology, Pleasanton CA, USA, Catalog # M05546-1)
Host
Rabbit
Contents
50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Clonality
Monoclonal
Clone Number
RM379
Isotype
Rabbit IgG
Immunogen
A peptide corresponding to human Terminal deoxynucleotidyl transferase (TdT)
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
This antibody reacts to human Terminal deoxynucleotidyl transferase (TdT)
Reactive Species
M05546-1 is reactive to DNTT in Human
Reconstitution
Calculated molecular weight
58536 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M05546-1 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (IHC): 1:100-1:200 dilution
WB: 1:1000-1:2000 dilution.
Validation Images & Assay Conditions
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Figure 1. Western Blotting result
Western Blot of Jurkat cells lysate using anti-TdT rabbit monoclonal antibody (Clone RM379) at a 1:2000 dilution.
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Figure 2. IHC result
Immunohistochemical staining of formalin fixed and paraffin embedded human thymus tissue section using anti-TdT rabbit monoclonal antibody (Clone RM379) at a 1:200 dilution.
Protein Target Info & Infographic
Gene/Protein Information For DNTT (Source: Uniprot.org, NCBI)
Gene Name
DNTT
Full Name
DNA nucleotidylexotransferase
Weight
58536 MW
Superfamily
DNA polymerase type-X family
Alternative Names
DNA nucleotidylexotransferase;2.7.7.31 ;Terminal addition enzyme;Terminal deoxynucleotidyltransferase;Terminal transferase ;DNTT;TDT ; DNTT TDT DNA nucleotidylexotransferase DNA nucleotidylexotransferase|nucleosidetriphosphate:DNA deoxynucleotidylexotransferase|terminal addition enzyme|terminal deoxynucleotidyltransferase|terminal deoxyribonucleotidyltransferase|terminal transferase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on DNTT, check out the DNTT Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for DNTT: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Terminal deoxynucleotidyl transferase (TdT) DNTT Rabbit Monoclonal Antibody, Clone#RM379 (M05546-1)
Hello CJ!
M05546-1 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Jiang Y, Yu M, Hu X, Han L, Yang K, Ba H, Zhang Z, Yin B, Yang XP, Li Z, Wang J. Cell Death Differ. 2017 Apr;24(4):660-671. doi: 10.1038/cdd.2016.162. Epub 2017 Feb 10. STAT1 mediates transmembrane TNF-alpha-induced formation of death-inducing sig...
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Customer Q&As
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5 Customer Q&As for Anti-Terminal deoxynucleotidyl transferase (TdT) DNTT Rabbit Monoclonal Antibody, Clone#RM379
Question
My colleagues were well pleased with the WB result of your anti-Terminal deoxynucleotidyl transferase (TdT) Rabbit Monoclonal antibody, Clone
Verified Customer
Verified customer
Asked: 2020-05-08
Answer
From what I have seen in literature, thymus does express DNTT. Generally DNTT expresses in nucleus. Regarding which tissues have DNTT expression, here are a few articles citing expression in various tissues:
Testis, Pubmed ID: 15489334
Thymus, Pubmed ID: 11473582, 15164054
Boster Scientific Support
Answered: 2020-05-08
Question
We have tried in the past anti-Terminal deoxynucleotidyl transferase (TdT) Rabbit Monoclonal antibody, Clone
Verified Customer
Verified customer
Asked: 2019-11-22
Answer
I took a look at the website and datasheets of our anti-Terminal deoxynucleotidyl transferase (TdT) Rabbit Monoclonal antibody, Clone
Boster Scientific Support
Answered: 2019-11-22
Question
We are currently using anti-Terminal deoxynucleotidyl transferase (TdT) Rabbit Monoclonal antibody, Clone#RM379 M05546-1 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on canine tissues as well?
Verified Customer
Verified customer
Asked: 2019-11-13
Answer
The anti-Terminal deoxynucleotidyl transferase (TdT) Rabbit Monoclonal antibody, Clone#RM379 (M05546-1) has not been tested for cross reactivity specifically with canine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-11-13
Question
We have been able to see staining in human bone marrow. Any tips? Is anti-Terminal deoxynucleotidyl transferase (TdT) Rabbit Monoclonal antibody, Clone#RM379 supposed to stain bone marrow positively?
R. Li
Verified customer
Asked: 2015-11-18
Answer
From literature bone marrow does express DNTT. From Uniprot.org, DNTT is expressed in bone marrow, thymus, testis, among other tissues. Regarding which tissues have DNTT expression, here are a few articles citing expression in various tissues:
Testis, Pubmed ID: 15489334
Thymus, Pubmed ID: 11473582, 15164054
Boster Scientific Support
Answered: 2015-11-18
Question
I am looking for using your anti-Terminal deoxynucleotidyl transferase (TdT) Rabbit Monoclonal antibody, Clone#RM379 for base-excision repair studies. Has this antibody been tested with western blotting on jurkat cells? We would like to see some validation images before ordering.
G. Roberts
Verified customer
Asked: 2015-11-04
Answer
We appreciate your inquiry. This M05546-1 anti-Terminal deoxynucleotidyl transferase (TdT) Rabbit Monoclonal antibody, Clone#RM379 is tested on jurkat cells, human thymus tissue. It is guaranteed to work for IHC, WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2015-11-04
