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Product Info Summary
| SKU: | M00154-1 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, WB |
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Product info
Product Name
Anti-CD19 Rabbit Monoclonal Antibody
SKU/Catalog Number
M00154-1
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-CD19 Rabbit Monoclonal Antibody catalog # M00154-1. Tested in WB, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CD19 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00154-1)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
AOEB-3
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human CD19
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00154-1 is reactive to CD19 in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
119 kDa
Calculated molecular weight
61128 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00154-1 is guaranteed for Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:1000
FC 1:50
Validation Images & Assay Conditions
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All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.
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All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.
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Western blot analysis of CD19 expression in (1) Jurkat cell lysate; (2) Rat spleen lysate.
Protein Target Info & Infographic
Gene/Protein Information For CD19 (Source: Uniprot.org, NCBI)
Gene Name
CD19
Full Name
B-lymphocyte antigen CD19
Weight
61128 MW
Alternative Names
B-lymphocyte antigen CD19;B-lymphocyte surface antigen B4;Differentiation antigen CD19;T-cell surface antigen Leu-12;CD19;CD19; CD19 B4, CVID3 CD19 molecule B-lymphocyte CD19|B-lymphocyte surface B4|T-cell surface Leu-12|differentiation CD19
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CD19, check out the CD19 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CD19: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CD19 Rabbit Monoclonal Antibody (M00154-1)
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No publications found for M00154-1
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Customer Reviews
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Customer Q&As
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15 Customer Q&As for Anti-CD19 Rabbit Monoclonal Antibody
Question
We are currently using anti-CD19 Rabbit Monoclonal antibody M00154-1 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on feline tissues as well?
Verified Customer
Verified customer
Asked: 2019-12-23
Answer
The anti-CD19 Rabbit Monoclonal antibody (M00154-1) has not been validated for cross reactivity specifically with feline tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-12-23
Question
Please see the WB image, lot number and protocol we used for spleen using anti-CD19 Rabbit Monoclonal antibody M00154-1. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-11-19
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-11-19
Question
Our lab want to know about to test anti-CD19 Rabbit Monoclonal antibody M00154-1 on human spleen for research purposes, then I may be interested in using anti-CD19 Rabbit Monoclonal antibody M00154-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-10-02
Answer
The products we sell, including anti-CD19 Rabbit Monoclonal antibody M00154-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-10-02
Question
Is a blocking peptide available for product anti-CD19 Rabbit Monoclonal antibody (M00154-1)?
Verified Customer
Verified customer
Asked: 2019-08-27
Answer
We do provide the blocking peptide for product anti-CD19 Rabbit Monoclonal antibody (M00154-1). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-08-27
Question
We have observed staining in mouse blood. Any tips? Is anti-CD19 Rabbit Monoclonal antibody supposed to stain blood positively?
Verified Customer
Verified customer
Asked: 2019-08-12
Answer
According to literature blood does express CD19. According to Uniprot.org, CD19 is expressed in spleen, tonsil, blood, b-cell, embryonic kidney, among other tissues. Regarding which tissues have CD19 expression, here are a few articles citing expression in various tissues:
B-cell, Pubmed ID: 15489334
Blood, Pubmed ID: 1370948
Embryonic kidney, Pubmed ID: 17525332
Spleen, Pubmed ID: 14702039
Tonsil, Pubmed ID: 2472450, 1375324
Boster Scientific Support
Answered: 2019-08-12
Question
Is this M00154-1 anti-CD19 Rabbit Monoclonal antibody reactive to the isotypes of CD19?
Verified Customer
Verified customer
Asked: 2019-08-02
Answer
The immunogen of M00154-1 anti-CD19 Rabbit Monoclonal antibody is A synthesized peptide derived from human CD19. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-08-02
Question
Is there a BSA free version of anti-CD19 Rabbit Monoclonal antibody M00154-1 available?
Verified Customer
Verified customer
Asked: 2018-12-03
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-CD19 Rabbit Monoclonal antibody M00154-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-12-03
Question
We need using your anti-CD19 Rabbit Monoclonal antibody for antigen receptor-mediated signaling pathway studies. Has this antibody been tested with western blotting on jurkat cell lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2018-11-29
Answer
We appreciate your inquiry. This M00154-1 anti-CD19 Rabbit Monoclonal antibody is validated on jurkat cell lysate. It is guaranteed to work for WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2018-11-29
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for spleen using anti-CD19 Rabbit Monoclonal antibody M00154-1. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2018-08-13
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-08-13
Question
Will anti-CD19 Rabbit Monoclonal antibody M00154-1 work for WB with spleen?
Verified Customer
Verified customer
Asked: 2018-08-09
Answer
According to the expression profile of spleen, CD19 is highly expressed in spleen. So, it is likely that anti-CD19 Rabbit Monoclonal antibody M00154-1 will work for WB with spleen.
Boster Scientific Support
Answered: 2018-08-09
Question
I was wanting to use your anti-CD19 Rabbit Monoclonal antibody for WB for human spleen on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human spleen identification?
Verified Customer
Verified customer
Asked: 2018-05-17
Answer
As indicated on the product datasheet, M00154-1 anti-CD19 Rabbit Monoclonal antibody has been tested for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human spleen in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-05-17
Question
I see that the anti-CD19 Rabbit Monoclonal antibody M00154-1 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2017-09-12
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2017-09-12
Question
My question regarding product M00154-1, anti-CD19 Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
S. Kulkarni
Verified customer
Asked: 2014-03-07
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00154-1 anti-CD19 Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2014-03-07
Question
Does M00154-1 anti-CD19 Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
A. Johnson
Verified customer
Asked: 2014-02-03
Answer
As indicated on the product datasheet, M00154-1 anti-CD19 Rabbit Monoclonal antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2014-02-03
Question
My boss were content with the WB result of your anti-CD19 Rabbit Monoclonal antibody. However we have seen positive staining in spleen cell membrane using this antibody. Is that expected? Could you tell me where is CD19 supposed to be expressed?
L. Li
Verified customer
Asked: 2013-02-20
Answer
Based on literature, spleen does express CD19. Generally CD19 expresses in cell membrane. Regarding which tissues have CD19 expression, here are a few articles citing expression in various tissues:
B-cell, Pubmed ID: 15489334
Blood, Pubmed ID: 1370948
Embryonic kidney, Pubmed ID: 17525332
Spleen, Pubmed ID: 14702039
Tonsil, Pubmed ID: 2472450, 1375324
Boster Scientific Support
Answered: 2013-02-20
