Click image to see more details
Product Info Summary
| SKU: | FC00154-PerCP |
|---|---|
| Size: | 100 tests, 200 tests |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | Flow Cytometry |
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Product info
Product Name
Anti-human CD19 Monoclonal Antibody PerCP Conjugated, Flow Validated
SKU/Catalog Number
FC00154-PerCP
Size
100 tests, 200 tests
Form
Liquid
Description
Boster Bio Anti-human CD19 Monoclonal Antibody PerCP Conjugated, Flow Validated (Catalog# FC00154-PerCP). Tested in Flow Cytometry application(s). This antibody reacts with Human.
Storage & Handling
Store at 2-8°C. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-human CD19 Monoclonal Antibody PerCP Conjugated, Flow Validated (Boster Biological Technology, Pleasanton CA, USA, Catalog # FC00154-PerCP)
Host
Mouse
Contents
PBS pH 7.2, 0.2% (w/v) BSA, 0.09% (w/v) sodium azide
Clonality
Monoclonal
Clone Number
Clone: 4G7
Isotype
IgG1,k
Immunogen
Human CCL (chronic lymphocytic leukemia) cells
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
FC00154-PerCP is reactive to CD19 in Human
Reconstitution
Observed Molecular Weight
68 kDa
Calculated molecular weight
61128 MW
Background of CD19
B-lymphocyte antigen CD19, also known as CD19 (Cluster of Differentiation 19), is a protein that in humans is encoded by the CD19 gene. It is found on the surface of B-cells, a type of white blood cell. Lymphocytes proliferate and differentiate in response to various concentrations of different antigens. The ability of the B cell to respond in a specific, yet sensitive manner to the various antigens is achieved with the use of low-affinity antigen receptors. The CD19 gene encodes a cell surface molecule that assembles with the antigen receptor of B lymphocytes in order to decrease the threshold for antigen receptor-dependent stimulation.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
FC00154-PerCP is guaranteed for Flow Cytometry Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
User needs to optimize the dilution ratio for this antibody.
Validation Images & Assay Conditions
Click image to see more details
Lymphocytes gated human PBMCs stained with PerCP conjugated anti-human CD19 (clone 4G7, red histogram). PBMCs stained with MOPC-31C IgG1 PerCP isotype control (Blue histogram).
Protein Target Info & Infographic
Gene/Protein Information For CD19 (Source: Uniprot.org, NCBI)
Gene Name
CD19
Full Name
B-lymphocyte antigen CD19
Weight
61128 MW
Alternative Names
B-lymphocyte antigen CD19;B-lymphocyte surface antigen B4;Differentiation antigen CD19;T-cell surface antigen Leu-12;CD19;CD19; CD19 B4, CVID3 CD19 molecule B-lymphocyte CD19|B-lymphocyte surface B4|T-cell surface Leu-12|differentiation CD19
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CD19, check out the CD19 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CD19: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-human CD19 Monoclonal Antibody PerCP Conjugated, Flow Validated (FC00154-PerCP)
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No publications found for FC00154-PerCP
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Customer Reviews
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Customer Q&As
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10 Customer Q&As for Anti-human CD19 Monoclonal Antibody PerCP Conjugated, Flow Validated
Question
See below the WB image, lot number and protocol we used for blood using anti-human CD19 Monoclonal antibody PerCP Conjugated, Flow Validated FC00154-PerCP. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-03-09
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-03-09
Question
Is a blocking peptide available for product anti-human CD19 Monoclonal antibody PerCP Conjugated, Flow Validated (FC00154-PerCP)?
Verified Customer
Verified customer
Asked: 2019-09-10
Answer
We do provide the blocking peptide for product anti-human CD19 Monoclonal antibody PerCP Conjugated, Flow Validated (FC00154-PerCP). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-09-10
Question
I was wanting to use your anti-human CD19 Monoclonal antibody PerCP Conjugated, Flow Validated for Flow Cytometry for human blood on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human blood identification?
Verified Customer
Verified customer
Asked: 2019-07-23
Answer
You can see on the product datasheet, FC00154-PerCP anti-human CD19 Monoclonal antibody PerCP Conjugated, Flow Validated has been validated for Flow Cytometry on human tissues. We have an innovator award program that if you test this antibody and show it works in human blood in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-07-23
Question
Does anti-human CD19 Monoclonal antibody PerCP Conjugated, Flow Validated FC00154-PerCP work for Flow Cytometry with blood?
Verified Customer
Verified customer
Asked: 2019-07-15
Answer
According to the expression profile of blood, CD19 is highly expressed in blood. So, it is likely that anti-human CD19 Monoclonal antibody PerCP Conjugated, Flow Validated FC00154-PerCP will work for Flow Cytometry with blood.
Boster Scientific Support
Answered: 2019-07-15
Question
Will FC00154-PerCP anti-human CD19 Monoclonal antibody PerCP Conjugated, Flow Validated work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-06-13
Answer
It shows on the product datasheet, FC00154-PerCP anti-human CD19 Monoclonal antibody PerCP Conjugated, Flow Validated as been tested on Flow Cytometry. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-06-13
Question
I see that the anti-human CD19 Monoclonal antibody PerCP Conjugated, Flow Validated FC00154-PerCP works with Flow Cytometry, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2018-04-19
Answer
You can find protocols for Flow Cytometry on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2018-04-19
Question
My question regarding product FC00154-PerCP, anti-human CD19 Monoclonal antibody PerCP Conjugated, Flow Validated. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2017-08-01
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free FC00154-PerCP anti-human CD19 Monoclonal antibody PerCP Conjugated, Flow Validated, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-08-01
Question
Is there a BSA free version of anti-human CD19 Monoclonal antibody PerCP Conjugated, Flow Validated FC00154-PerCP available?
G. Dhar
Verified customer
Asked: 2017-05-08
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-human CD19 Monoclonal antibody PerCP Conjugated, Flow Validated FC00154-PerCP are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2017-05-08
Question
I am interested in to test anti-human CD19 Monoclonal antibody PerCP Conjugated, Flow Validated FC00154-PerCP on human blood for research purposes, then I may be interested in using anti-human CD19 Monoclonal antibody PerCP Conjugated, Flow Validated FC00154-PerCP for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
P. Carter
Verified customer
Asked: 2015-05-26
Answer
The products we sell, including anti-human CD19 Monoclonal antibody PerCP Conjugated, Flow Validated FC00154-PerCP, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2015-05-26
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for blood using anti-human CD19 Monoclonal antibody PerCP Conjugated, Flow Validated FC00154-PerCP. Let me know if you need anything else.
C. Krishna
Verified customer
Asked: 2015-02-02
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2015-02-02
