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Product Info Summary
| SKU: | A00198-1 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-Toll-like receptor 9 TLR9 Antibody
SKU/Catalog Number
A00198-1
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Toll-like receptor 9 TLR9 Antibody (Catalog # A00198-1). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse.
Storage & Handling
TLR9 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-Toll-like receptor 9 TLR9 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00198-1)
Host
Rabbit
Contents
TLR9 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
TLR9 antibody was raised against a peptide corresponding to 16 amino acids near the carboxy terminus of human TLR9. The immunogen is located within amino acids 960 - 1010 of TLR9.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
ITCH antibody is human, mouse and rat reactive. At least three isoforms of ITCH are known to exist; this antibody only recognizes the two longest isoforms. This antibody is predicted to not cross-react with other members of the Nedd4 protein family.
Reactive Species
A00198-1 is reactive to TLR9 in Human, Mouse
Reconstitution
Observed Molecular Weight
68 kDa
Calculated molecular weight
115860 MW
Background of TLR9
Toll-like receptors (TLRs) are evolutionarily conserved pattern-recognition molecules resembling the toll proteins that mediate antimicrobial responses in Drosophila. These proteins recognize different microbial products during infection and serve as an important link between the innate and adaptive immune responses. TLR9 forms a subfamily along with TLR7 and TLR8 that recognize viral RNA and CpG DNA sequences and are localized in intracellular acidic compartments such as the phagolysosome. Unlike other TLRs which act through adaptor molecules such as TOLLIP, TIRAP, TRIF, and MyD88 to activate various kinases and transcription factors to respond to potential infection, TLR9 is strictly dependent on MyD88.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00198-1 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
TLR9 antibody can be used for detection of TLR9 by Western blot at 0.5 to 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2 μg/mL. For immunofluorescence start at 10 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of TLR9 in mouse spleen cell lysate with TLR9 antibody at (A) 0.5 and (B) 1 μg/mL.
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Immunohistochemistry of TLR9 in mouse spleen cells with TLR9 antibody at 2 μg/mL.
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Immunofluorescence of TLR9 in mouse spleen cells with TLR9 antibody at 10 μg/mL.
Protein Target Info & Infographic
Gene/Protein Information For TLR9 (Source: Uniprot.org, NCBI)
Gene Name
TLR9
Full Name
Toll-like receptor 9
Weight
115860 MW
Superfamily
Toll-like receptor family
Alternative Names
CD289, Toll-like receptor 9 TLR9 CD289 toll like receptor 9 toll-like receptor 9
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TLR9, check out the TLR9 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TLR9: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Toll-like receptor 9 TLR9 Antibody (A00198-1)
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