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Product Info Summary
| SKU: | A00154-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-CD19 Antibody Picoband®
SKU/Catalog Number
A00154-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-CD19 Antibody Picoband® catalog # A00154-1. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CD19 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00154-1)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human CD19 recombinant protein (Position: P20-Y259).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A00154-1 is reactive to CD19 in Human, Mouse
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
85 kDa
Calculated molecular weight
61.128kDa
Background of CD19
B-lymphocyte antigen CD19, also known as CD19 (Cluster of Differentiation 19), is a protein that in humans is encoded by the CD19 gene. It is found on the surface of B-cells, a type of white blood cell. Lymphocytes proliferate and differentiate in response to various concentrations of different antigens. The ability of the B cell to respond in a specific, yet sensitive manner to the various antigens is achieved with the use of low-affinity antigen receptors. The CD19 gene encodes a cell surface molecule that assembles with the antigen receptor of B lymphocytes in order to decrease the threshold for antigen receptor-dependent stimulation.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00154-1 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
ELISA, 0.1-0.5μg/ml
Positive Control
WB: mouse spleen tissue, mouse thymus tissue
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of CD19 using anti-CD19 antibody (A00154-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: mouse spleen tissue lysates,
Lane 2: mouse thymus tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD19 antigen affinity purified polyclonal antibody (Catalog # A00154-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD19 at approximately 85KD. The expected band size for CD19 is at 61KD.
Protein Target Info & Infographic
Gene/Protein Information For CD19 (Source: Uniprot.org, NCBI)
Gene Name
CD19
Full Name
B-lymphocyte antigen CD19
Weight
61.128kDa
Alternative Names
B-lymphocyte antigen CD19; B-lymphocyte surface antigen B4; Differentiation antigen CD19; T-cell surface antigen Leu-12; CD19; CD19 CD19 B4, CVID3 CD19 molecule B-lymphocyte CD19|B-lymphocyte surface B4|T-cell surface Leu-12|differentiation CD19
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CD19, check out the CD19 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CD19: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CD19 Antibody Picoband® (A00154-1)
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No publications found for A00154-1
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Customer Q&As
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16 Customer Q&As for Anti-CD19 Antibody Picoband®
Question
Is a blocking peptide available for product anti-CD19 antibody (A00154-1)?
J. Krishna
Verified customer
Asked: 2019-11-21
Answer
We do provide the blocking peptide for product anti-CD19 antibody (A00154-1). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-11-21
Question
Is this A00154-1 anti-CD19 antibody reactive to the isotypes of CD19?
Verified Customer
Verified customer
Asked: 2019-11-20
Answer
The immunogen of A00154-1 anti-CD19 antibody is E. coli-derived human CD19 recombinant protein (Position: P20-Y259). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-11-20
Question
We bought anti-CD19 antibody for ELISA on tonsil in a previous project. I am using mouse, and We want to use the antibody for WB next. I was wanting to use examining tonsil as well as b-cell in our next experiment. Could you please give me some suggestion on which antibody would work the best for WB?
Verified Customer
Verified customer
Asked: 2019-08-26
Answer
I looked at the website and datasheets of our anti-CD19 antibody and it appears that A00154-1 has been tested on mouse in both ELISA and WB. Thus A00154-1 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-08-26
Question
We are currently using anti-CD19 antibody A00154-1 for mouse tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it possible that the antibody can work on pig tissues as well?
Verified Customer
Verified customer
Asked: 2019-04-16
Answer
The anti-CD19 antibody (A00154-1) has not been validated for cross reactivity specifically with pig tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-04-16
Question
My team were happy with the WB result of your anti-CD19 antibody. However we have been able to see positive staining in embryonic kidney cell membrane using this antibody. Is that expected? Could you tell me where is CD19 supposed to be expressed?
G. Krishna
Verified customer
Asked: 2019-02-08
Answer
From literature, embryonic kidney does express CD19. Generally CD19 expresses in cell membrane. Regarding which tissues have CD19 expression, here are a few articles citing expression in various tissues:
B-cell, Pubmed ID: 15489334
Blood, Pubmed ID: 1370948
Embryonic kidney, Pubmed ID: 17525332
Spleen, Pubmed ID: 14702039
Tonsil, Pubmed ID: 2472450, 1375324
Boster Scientific Support
Answered: 2019-02-08
Question
Is there a BSA free version of anti-CD19 antibody A00154-1 available?
Verified Customer
Verified customer
Asked: 2018-12-20
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-CD19 antibody A00154-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-12-20
Question
Will anti-CD19 antibody A00154-1 work for ELISA with embryonic kidney?
Verified Customer
Verified customer
Asked: 2018-11-27
Answer
According to the expression profile of embryonic kidney, CD19 is highly expressed in embryonic kidney. So, it is likely that anti-CD19 antibody A00154-1 will work for ELISA with embryonic kidney.
Boster Scientific Support
Answered: 2018-11-27
Question
I have attached the WB image, lot number and protocol we used for embryonic kidney using anti-CD19 antibody A00154-1. Please let me know if you require anything else.
K. Williams
Verified customer
Asked: 2018-11-13
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-11-13
Question
I see that the anti-CD19 antibody A00154-1 works with ELISA, what is the protocol used to produce the result images on the product page?
F. Williams
Verified customer
Asked: 2018-08-07
Answer
You can find protocols for ELISA on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2018-08-07
Question
I have a question about product A00154-1, anti-CD19 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2017-12-13
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00154-1 anti-CD19 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-12-13
Question
I am interested in using your anti-CD19 antibody for pp2a and ier3 regulate pi3k/akt signaling studies. Has this antibody been tested with western blotting on mouse thymus tissue? We would like to see some validation images before ordering.
J. Johnson
Verified customer
Asked: 2016-10-13
Answer
Thank you for your inquiry. This A00154-1 anti-CD19 antibody is tested on mouse thymus tissue, spleen tissue. It is guaranteed to work for ELISA, WB in human, mouse. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2016-10-13
Question
I was wanting to use your anti-CD19 antibody for ELISA for mouse embryonic kidney on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse embryonic kidney identification?
G. Walker
Verified customer
Asked: 2015-06-30
Answer
It shows on the product datasheet, A00154-1 anti-CD19 antibody has been tested for ELISA, WB on human, mouse tissues. We have an innovator award program that if you test this antibody and show it works in mouse embryonic kidney in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2015-06-30
Question
My question regards to test anti-CD19 antibody A00154-1 on mouse embryonic kidney for research purposes, then I may be interested in using anti-CD19 antibody A00154-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
P. Brown
Verified customer
Asked: 2015-02-23
Answer
The products we sell, including anti-CD19 antibody A00154-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2015-02-23
Question
Would A00154-1 anti-CD19 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
J. Bhatt
Verified customer
Asked: 2014-12-08
Answer
It shows on the product datasheet, A00154-1 anti-CD19 antibody as been tested on ELISA. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2014-12-08
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for embryonic kidney using anti-CD19 antibody A00154-1. Let me know if you need anything else.
A. Banerjee
Verified customer
Asked: 2014-12-05
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2014-12-05
Question
We have observed staining in mouse spleen. Are there any suggestions? Is anti-CD19 antibody supposed to stain spleen positively?
Z. Collins
Verified customer
Asked: 2013-11-14
Answer
According to literature spleen does express CD19. According to Uniprot.org, CD19 is expressed in spleen, tonsil, blood, b-cell, embryonic kidney, among other tissues. Regarding which tissues have CD19 expression, here are a few articles citing expression in various tissues:
B-cell, Pubmed ID: 15489334
Blood, Pubmed ID: 1370948
Embryonic kidney, Pubmed ID: 17525332
Spleen, Pubmed ID: 14702039
Tonsil, Pubmed ID: 2472450, 1375324
Boster Scientific Support
Answered: 2013-11-14
