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Product Info Summary
| SKU: | PB9800 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-CD19 Antibody Picoband®
SKU/Catalog Number
PB9800
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-CD19 Antibody Picoband® catalog # PB9800. Tested in IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CD19 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9800)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human CD19, different from the related mouse sequence by seven amino acids.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
PB9800 is reactive to CD19 in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
95 kDa
Calculated molecular weight
61128 MW
Background of CD19
B-lymphocyte antigen CD19, also known as CD19 (Cluster of Differentiation 19), is a protein that in humans is encoded by the CD19 gene. It is found on the surface of B-cells, a type of white blood cell. Lymphocytes proliferate and differentiate in response to various concentrations of different antigens. The ability of the B cell to respond in a specific, yet sensitive manner to the various antigens is achieved with the use of low-affinity antigen receptors. The CD19 gene encodes a cell surface molecule that assembles with the antigen receptor of B lymphocytes in order to decrease the threshold for antigen receptor-dependent stimulation.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9800 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human, By Heat
Positive Control
WB: human Raji whole cell, human Ramos whole cell, human Daudi whole cell
IHC: human appendix tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of CD19 using anti-CD19 antibody (PB9800).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Raji whole cell lysates,
Lane 2: human Ramos whole cell lysates,
Lane 3: human Daudi whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD19 antigen affinity purified polyclonal antibody (Catalog # PB9800) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD19 at approximately 95 kDa. The expected band size for CD19 is at 61 kDa.
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Figure 2. IHC analysis of CD19 using anti-CD19 antibody (PB9800).
CD19 was detected in a paraffin-embedded section of human appendix tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CD19 Antibody (PB9800) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For CD19 (Source: Uniprot.org, NCBI)
Gene Name
CD19
Full Name
B-lymphocyte antigen CD19
Weight
61128 MW
Alternative Names
B-lymphocyte antigen CD19;B-lymphocyte surface antigen B4;Differentiation antigen CD19;T-cell surface antigen Leu-12;CD19;CD19; CD19 B4, CVID3 CD19 molecule B-lymphocyte CD19|B-lymphocyte surface B4|T-cell surface Leu-12|differentiation CD19
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CD19, check out the CD19 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CD19: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CD19 Antibody Picoband® (PB9800)
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No publications found for PB9800
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Customer Q&As
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5 Customer Q&As for Anti-CD19 Antibody Picoband®
Question
Our lab used your anti-CD19 antibody for IHC on b-cell in a previous project. I am using human, and We are going to use the antibody for WB next. I would like examining b-cell as well as blood in our next experiment. Could you please give me some suggestion on which antibody would work the best for WB?
Verified Customer
Verified customer
Asked: 2020-01-16
Answer
I have checked the website and datasheets of our anti-CD19 antibody and I see that PB9800 has been tested on human in both IHC and WB. Thus PB9800 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2020-01-16
Question
My lab would like using your anti-CD19 antibody for antigen activates b cell receptor (bcr) leading to generation of second messengers studies. Has this antibody been tested with western blotting on sw620 whole cell lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-12-26
Answer
We appreciate your inquiry. This PB9800 anti-CD19 antibody is tested on raji whole cell lysate, a549 whole cell lysate, sw620 whole cell lysate. It is guaranteed to work for IHC, WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-12-26
Question
We were content with the WB result of your anti-CD19 antibody. However we have observed positive staining in tonsil cell membrane using this antibody. Is that expected? Could you tell me where is CD19 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-09-27
Answer
Based on literature, tonsil does express CD19. Generally CD19 expresses in cell membrane. Regarding which tissues have CD19 expression, here are a few articles citing expression in various tissues:
B-cell, Pubmed ID: 15489334
Blood, Pubmed ID: 1370948
Embryonic kidney, Pubmed ID: 17525332
Spleen, Pubmed ID: 14702039
Tonsil, Pubmed ID: 2472450, 1375324
Boster Scientific Support
Answered: 2019-09-27
Question
We have observed staining in human tonsil. Any tips? Is anti-CD19 antibody supposed to stain tonsil positively?
Verified Customer
Verified customer
Asked: 2019-08-07
Answer
From literature tonsil does express CD19. From Uniprot.org, CD19 is expressed in spleen, tonsil, blood, b-cell, embryonic kidney, among other tissues. Regarding which tissues have CD19 expression, here are a few articles citing expression in various tissues:
B-cell, Pubmed ID: 15489334
Blood, Pubmed ID: 1370948
Embryonic kidney, Pubmed ID: 17525332
Spleen, Pubmed ID: 14702039
Tonsil, Pubmed ID: 2472450, 1375324
Boster Scientific Support
Answered: 2019-08-07
Question
We are currently using anti-CD19 antibody PB9800 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on monkey tissues as well?
E. Yang
Verified customer
Asked: 2017-12-12
Answer
The anti-CD19 antibody (PB9800) has not been tested for cross reactivity specifically with monkey tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-12-12
