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Product Info Summary
| SKU: | PA1016-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, WB |
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Product info
Product Name
Anti-C-C chemokine receptor type 5 CCR5 Antibody Picoband®
SKU/Catalog Number
PA1016-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-C-C chemokine receptor type 5 CCR5 Antibody catalog # PA1016-1. Tested in Flow Cytometry, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-C-C chemokine receptor type 5 CCR5 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1016-1)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human CCR5.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1016-1 is reactive to CCR5 in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
40 kDa
Calculated molecular weight
40524 MW
Background of CCR5
Human CC chemokine receptor 5 (CCR5), which is 17.5 kb from the CMKBR2 gene, encodes a 352-amino acid protein with a calculated molecular mass of 40,600 Da. It is organized into four exons and two introns. Human CCR5 is a co-receptor for macrophage-tropic strains of human immunodeficiency virus (HIV)-1 and is expressed by bone-marrow-derived cells. CCR5 is expressed on neurons, astrocytes, and microglia in the central nervous system, and on epithelium, endothelium, vascular smooth muscle and fibroblasts in other tissues. Functionally, CCR5 mediates the activation of cells by the chemokines macrophage inflammatory protein-1alpha, macrophage inflammatory protein-1beta, and RANTES, and serves as a fusion cofactor for macrophage-tropic strains of human immunodeficiency virus type 1.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1016-1 is guaranteed for Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Flow Cytometry (Fixed), 1-3μg/1x106 cells, Human
Positive Control
WB: COLO320 Cell, MCF-7 Cell, SMMC Cell, JURKAT Cell
Validation Images & Assay Conditions
Click image to see more details
Anti-CCR5 antibody, PA1016-1, Western blotting
Lane 1: COLO320 Cell Lysate
Lane 2: MCF-7 Cell Lysate
Lane 3: SMMC Cell Lysate
Lane 4: JURKAT Cell Lysate
Protein Target Info & Infographic
Gene/Protein Information For CCR5 (Source: Uniprot.org, NCBI)
Gene Name
CCR5
Full Name
C-C chemokine receptor type 5
Weight
40524 MW
Superfamily
G-protein coupled receptor 1 family
Alternative Names
C-C chemokine receptor type 5;C-C CKR-5;CC-CKR-5;CCR-5;CCR5;CHEMR13;HIV-1 fusion coreceptor;CD195;CCR5;CMKBR5; CCR5 CC-CKR-5, CCCKR5, CCR-5, CD195, CKR-5, CKR5, CMKBR5, IDDM22 C-C motif chemokine receptor 5 C-C chemokine receptor type 5|C-C motif chemokine receptor 5 A159A|HIV-1 fusion coreceptor|chemokine (C-C motif) receptor 5|chemokine receptor CCR5|chemokine recptor CCR5 Delta32|chemr13
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CCR5, check out the CCR5 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CCR5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-C-C chemokine receptor type 5 CCR5 Antibody Picoband® (PA1016-1)
Hello CJ!
PA1016-1 has been cited in 2 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Study on CCR5 analogs and affinity peptides
Li G, Xu H, Zhu S, Xu W, Qin S, Liu S, Tu G, Peng H, Qiu S, Yu S, Zhu Q, Fan B, Zheng C, Li G, Liang S. Brain Res Bull. 2013 Jan;90:79-87. Doi: 10.1016/J.Brainresbull.2012.10.002. Epub 2012 Oct 9. Effects Of Neferine On Ccl5 And Ccr5 Expression In...
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Customer Q&As
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5 Customer Q&As for Anti-C-C chemokine receptor type 5 CCR5 Antibody Picoband®
Question
We have observed staining in human visceral pleura. Are there any suggestions? Is anti-CCR5 antibody supposed to stain visceral pleura positively?
Verified Customer
Verified customer
Asked: 2019-08-21
Answer
Based on literature visceral pleura does express CCR5. Based on Uniprot.org, CCR5 is expressed in visceral pleura, macrophage, among other tissues. Regarding which tissues have CCR5 expression, here are a few articles citing expression in various tissues:
Macrophage, Pubmed ID: 8663314
Boster Scientific Support
Answered: 2019-08-21
Question
Our lab want to know about using your anti-CCR5 antibody for chemotaxis studies. Has this antibody been tested with western blotting on smmc cell lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-05-23
Answer
We appreciate your inquiry. This PA1016-1 anti-CCR5 antibody is tested on colo320 cell lysate, smmc cell lysate, jurkat cell lysate. It is guaranteed to work for Flow Cytometry, WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-05-23
Question
We were happy with the WB result of your anti-CCR5 antibody. However we have observed positive staining in macrophage cell membrane using this antibody. Is that expected? Could you tell me where is CCR5 supposed to be expressed?
B. Roberts
Verified customer
Asked: 2018-10-05
Answer
From what I have seen in literature, macrophage does express CCR5. Generally CCR5 expresses in cell membrane. Regarding which tissues have CCR5 expression, here are a few articles citing expression in various tissues:
Macrophage, Pubmed ID: 8663314
Boster Scientific Support
Answered: 2018-10-05
Question
We ordered your anti-CCR5 antibody for WB on visceral pleura in the past. I am using human, and We intend to use the antibody for Flow Cytometry next. I would like examining visceral pleura as well as macrophage in our next experiment. Could give a recommendation on which antibody would work the best for Flow Cytometry?
Verified Customer
Verified customer
Asked: 2018-09-24
Answer
I viewed the website and datasheets of our anti-CCR5 antibody and it appears that PA1016-1 has been tested on human in both WB and Flow Cytometry. Thus PA1016-1 should work for your application. Our Boster satisfaction guarantee will cover this product for Flow Cytometry in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for Flow Cytometry detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2018-09-24
Question
We are currently using anti-CCR5 antibody PA1016-1 for human tissue, and we are satisfied with the Flow Cytometry results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on pig tissues as well?
K. Dhar
Verified customer
Asked: 2017-04-25
Answer
The anti-CCR5 antibody (PA1016-1) has not been tested for cross reactivity specifically with pig tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-04-25
