Click image to see more details
Product Info Summary
| SKU: | PB9048 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-Cathepsin D/CTSD Antibody Picoband®
View all Cathepsin D Antibodies
SKU/Catalog Number
PB9048
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Cathepsin D/CTSD Antibody Picoband® catalog # PB9048. Tested in IHC, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Cathepsin D/CTSD Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9048)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived mouse Cathepsin D recombinant protein (Position: E65-L410). Mouse Cathepsin D shares 85% and 91% amino acid (aa) sequences identity with human and rat Cathepsin D, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9048 is reactive to Ctsd in Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
45 kDa
Calculated molecular weight
44954 MW
Background of Cathepsin D
Cathepsin D is a protein that in humans is encoded by the CTSD gene. This proteinase is a member of the peptidase C1 family, having a specificity similar to but narrower than that of pepsin A. It is mapped to 11p15.5. The cDNA encodes a 412-amino acid protein with 20 and 44 amino acids in a pre- and prosegment, respectively. Cathepsin D is one of the lysosomal proteinases. It is ubiquitously expressed and is involved in proteolytic degradation, cell invasion, and apoptosis. Mutations in this gene are involved in the pathogenesis of several diseases, including breast cancer and possibly Alzheimer disease and it has been considered as a breast cancer tumor marker.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9048 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Mouse
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Mouse, Rat, By Heat
Positive Control
WB: Mouse Liver Tissue, Mouse Brain Tissue, Mouse Thymus Tissue, NEURO Whole Cell,
IHC: Mouse Kidney tissue, Mouse Liver tissue, rat liver tissue
Validation Images & Assay Conditions
Click image to see more details
Figure 1. IHC analysis of Cathepsin D using anti-Cathepsin D antibody (PB9048).
Cathepsin D was detected in paraffin-embedded section of Mouse Kidney Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Cathepsin D Antibody (PB9048) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 2. IHC analysis of Cathepsin D using anti-Cathepsin D antibody (PB9048).
Cathepsin D was detected in paraffin-embedded section of Mouse Liver Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Cathepsin D Antibody (PB9048) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 3. Western blot analysis of Cathepsin D using anti-Cathepsin D antibody (PB9048).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: Mouse Liver Tissue Lysate
Lane 2: Mouse Brain Tissue Lysate
Lane 3: Mouse Thymus Tissue Lysate
Lane 4: NEURO Whole Cell Lysate
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Cathepsin D antigen affinity purified polyclonal antibody (Catalog # PB9048) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Cathepsin D at approximately 45KD. The expected band size for Cathepsin D is at 45KD.
Click image to see more details
Figure 4. IHC analysis of Cathepsin D using anti-Cathepsin D antibody (PB9048).
Cathepsin D was detected in paraffin-embedded section of rat liver tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Cathepsin D Antibody (PB9048) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For Ctsd (Source: Uniprot.org, NCBI)
Gene Name
Ctsd
Full Name
Cathepsin D
Weight
44954 MW
Superfamily
peptidase A1 family
Alternative Names
Cathepsin D;3.4.23.5;Ctsd; CTSD CLN10, CPSD, HEL-S-130P cathepsin D cathepsin D|ceroid-lipofuscinosis, neuronal 10|epididymis secretory sperm binding protein Li 130P|lysosomal aspartyl peptidase|lysosomal aspartyl protease
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Ctsd, check out the Ctsd Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Ctsd: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Cathepsin D/CTSD Antibody Picoband® (PB9048)
Hello CJ!
PB9048 has been cited in 2 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Ma WQ,Sun XJ,Zhu Y,Liu NF. PDK4 promotes vascular calcification by interfering with autophagic activity and metabolic reprogramming. Cell Death Dis.2020 Nov 17;11(11):991.doi:10.1038/s41419-020-03162-w. PMID:33203874;PMCID:PMC7673024.
Species: Rat
Inhibition of autophagosome-lysosome fusion by ginsenoside Ro via the ESR2-NCF1-ROS pathway sensitizes esophageal cancer cells to 5-fluorouracil-induced cell death via the CHEK1-mediated DNA damage checkpoint
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-Cathepsin D/CTSD Antibody Picoband®?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Anti-Cathepsin D/CTSD Antibody Picoband®
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
16 Customer Q&As for Anti-Cathepsin D/CTSD Antibody Picoband®
Question
We are currently using anti-Cathepsin D/CTSD antibody PB9048 for mouse tissue, and we are content with the WB results. The species of reactivity given in the datasheet says mouse, rat. Is it possible that the antibody can work on bovine tissues as well?
C. Miller
Verified customer
Asked: 2020-02-18
Answer
The anti-Cathepsin D/CTSD antibody (PB9048) has not been tested for cross reactivity specifically with bovine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-02-18
Question
I have attached the WB image, lot number and protocol we used for melanoma using anti-Cathepsin D/CTSD antibody PB9048. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-10-24
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-10-24
Question
We bought anti-Cathepsin D/CTSD antibody for WB on adult mammalian kidney last year. I am using rat, and We want to use the antibody for IHC-P next. I was wanting to use examining adult mammalian kidney as well as melanoma in our next experiment. Could you please give me some suggestion on which antibody would work the best for IHC-P?
Verified Customer
Verified customer
Asked: 2019-10-01
Answer
I viewed the website and datasheets of our anti-Cathepsin D/CTSD antibody and it appears that PB9048 has been validated on rat in both WB and IHC-P. Thus PB9048 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC-P in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC-P detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-10-01
Question
Is a blocking peptide available for product anti-Cathepsin D/CTSD antibody (PB9048)?
Verified Customer
Verified customer
Asked: 2019-09-25
Answer
We do provide the blocking peptide for product anti-Cathepsin D/CTSD antibody (PB9048). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-09-25
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for melanoma using anti-Cathepsin D/CTSD antibody PB9048. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-09-24
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-09-24
Question
Will PB9048 anti-Cathepsin D/CTSD antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-07-24
Answer
It shows on the product datasheet, PB9048 anti-Cathepsin D/CTSD antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-07-24
Question
I was wanting to use your anti-Cathepsin D/CTSD antibody for WB for rat melanoma on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat melanoma identification?
Verified Customer
Verified customer
Asked: 2019-06-24
Answer
As indicated on the product datasheet, PB9048 anti-Cathepsin D/CTSD antibody has been validated for IHC-P, WB on mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat melanoma in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-06-24
Question
My colleagues were happy with the WB result of your anti-Cathepsin D/CTSD antibody. However we have been able to see positive staining in melanoma lysosome. melanosome. secreted, using this antibody. Is that expected? Could you tell me where is CTSD supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-05-29
Answer
From what I have seen in literature, melanoma does express CTSD. Generally CTSD expresses in lysosome. melanosome. secreted,. Regarding which tissues have CTSD expression, here are a few articles citing expression in various tissues:
Kidney, Pubmed ID: 15489334
Liver, Pubmed ID: 1426530, 8393577, 19159218
Melanoma, Pubmed ID: 12643545, 17081065
Plasma, Pubmed ID: 16335952
Platelet, Pubmed ID: 16263699
Spleen, Pubmed ID: 8467789
Boster Scientific Support
Answered: 2019-05-29
Question
Can you help my question with product PB9048, anti-Cathepsin D/CTSD antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-04-16
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PB9048 anti-Cathepsin D/CTSD antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-04-16
Question
Is this PB9048 anti-Cathepsin D/CTSD antibody reactive to the isotypes of CTSD?
Verified Customer
Verified customer
Asked: 2019-02-21
Answer
The immunogen of PB9048 anti-Cathepsin D/CTSD antibody is E.coli-derived mouse Cathepsin D recombinant protein (Position: E65-L410). Mouse Cathepsin D shares 85% and 91% amino acid (aa) sequences identity with human and rat Cathepsin D, respectively. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-02-21
Question
Do you have a BSA free version of anti-Cathepsin D/CTSD antibody PB9048 available?
G. Wu
Verified customer
Asked: 2018-03-28
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-Cathepsin D/CTSD antibody PB9048 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-03-28
Question
We have been able to see staining in mouse melanoma. Do you have any suggestions? Is anti-Cathepsin D/CTSD antibody supposed to stain melanoma positively?
Verified Customer
Verified customer
Asked: 2018-03-28
Answer
Based on literature melanoma does express CTSD. Based on Uniprot.org, CTSD is expressed in adult mammalian kidney, kidney, liver, melanoma, plasma, platelet, spleen, among other tissues. Regarding which tissues have CTSD expression, here are a few articles citing expression in various tissues:
Kidney, Pubmed ID: 15489334
Liver, Pubmed ID: 1426530, 8393577, 19159218
Melanoma, Pubmed ID: 12643545, 17081065
Plasma, Pubmed ID: 16335952
Platelet, Pubmed ID: 16263699
Spleen, Pubmed ID: 8467789
Boster Scientific Support
Answered: 2018-03-28
Question
Will anti-Cathepsin D/CTSD antibody PB9048 work for WB with melanoma?
Verified Customer
Verified customer
Asked: 2017-06-20
Answer
According to the expression profile of melanoma, CTSD is highly expressed in melanoma. So, it is likely that anti-Cathepsin D/CTSD antibody PB9048 will work for WB with melanoma.
Boster Scientific Support
Answered: 2017-06-20
Question
I see that the anti-Cathepsin D/CTSD antibody PB9048 works with WB, what is the protocol used to produce the result images on the product page?
C. Miller
Verified customer
Asked: 2016-12-27
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2016-12-27
Question
We are interested in using your anti-Cathepsin D/CTSD antibody for r-mmu-1442490; collagen degradation studies. Has this antibody been tested with western blotting on neuro whole cell lysate? We would like to see some validation images before ordering.
H. Gonzalez
Verified customer
Asked: 2015-05-20
Answer
Thank you for your inquiry. This PB9048 anti-Cathepsin D/CTSD antibody is tested on mouse thymus tissue, kidney tissue, tissue lysate, liver tissue, brain tissue, neuro whole cell lysate. It is guaranteed to work for IHC-P, WB in mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2015-05-20
Question
My lab would like to test anti-Cathepsin D/CTSD antibody PB9048 on rat melanoma for research purposes, then I may be interested in using anti-Cathepsin D/CTSD antibody PB9048 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
V. Krishna
Verified customer
Asked: 2013-11-19
Answer
The products we sell, including anti-Cathepsin D/CTSD antibody PB9048, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2013-11-19
