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Key=store_id Key=entity_id, value=100506 Key=attribute_set_id, value=4 Key=type_id, value=simple Key=sku, value=FEK0672 Key=has_options, value=0 Key=required_options, value=0 Key=created_at, value=2021-03-08 07:08:29 Key=updated_at, value=2024-09-26 01:19:13 Key=mst_search_weight, value=0 Key=status, value=Enabled Key=name, value=Human Cathepsin D PicoKine® Quick ELISA Kit Key=cj_search_index Key=publication_count, value=0 Key=qty_in_warehouses Key=is_recurring, value=No Key=visibility, value=Catalog, Search Key=tax_class_id, value=Taxable Goods Key=featured, value=No Key=featured_image, value=Yes Key=fb_product, value= Key=custom_product_page, value=No Key=free_shipping, value=No Key=template, value=tag-quick-elisa-kits Key=quantity_and_stock_status Key=googleshopping_exclude, value=No Key=yoast_keyword_score, value=-1365 Key=yoast_content_score, value=60 Key=price, value=499.0000 Key=weight, value=1.0000 Key=meta_title, value=Human Cathepsin D PicoKine® Quick ELISA Kit | Boster Bio Key=meta_description, value=Human Cathepsin D PicoKine™ Quick ELISA Kit (FEK0672) is a quantitative ELISA for measuring Cathepsin D in human cell culture supernatants, cell lysates, serum & plasma. Key=image, value=no_selection Key=small_image, value=no_selection Key=thumbnail, value=no_selection Key=url_key, value=human-cathepsin-d-picokine-trade-quick-elisa-kit-fek0672-boster Key=url_path, value=human-cystatin-c-picokine-trade-elisa-kit-ek0678-boster Key=options_container, value=Product Info Column Key=msrp_display_actual_price_type, value= Key=gift_message_available, value=No Key=size, value=96 wells/kit, with removable strips. Key=protein_name, value=Cathepsin D Key=gene_full_name, value=cathepsin D Key=uniprot_id, value=P07339 Key=assay_range, value=156 pg/ml - 10,000 pg/ml Key=sensitivity, value=<10 pg/ml Key=applications, value=ELISA Key=reactivity, value=Human Key=cross_reactivity, value=There is no detectable cross-reactivity with other relevant proteins. Key=principle, value=polyclonal antibody from goat|polyclonal antibody from goat Key=anticoagulant, value=heparin, EDTA Key=assay_procedure_step_1, value=10,000 pg/ml, 5,000 pg/ml, 2,500 pg/ml, 1,250 pg/ml, 625 pg/ml, 312.5 pg/ml, 156.25 pg/ml Key=source_company, value=Boster FEK0672 Key=product_category, value=Picokine ELISA Kits, Fast ELISA Kits Key=research_category, value=Cardiovascular, Caspases, Cell Biology, Cell Type Markers, Neural Stem Cells, Stem Cells, Tags & Cell Markers, Tumor Associated Key=swatch_image, value=no_selection Key=rating_value, value=100 Key=review_count, value=10 Key=mp_exclude_sitemap, value=Yes Key=cj_related_products, value=Neuronal Ceroid-lipofuscinoses:PB9047|PB9048|A01361|A07500|PB9781|M02690 Key=description, value=Human Cathepsin D PicoKine® Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Human CTSD in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays. Key=short_description, value=Human Cathepsin D PicoKine® Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Human CTSD in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays. Key=meta_keyword, value=Human Cathepsin D Quick ELISA Kit Key=background, value=Cathepsin D is a protein that in humans is encoded by the CTSD gene. This proteinase is a member of the peptidase C1 family, having a specificity similar to but narrower than that of pepsin A. It is mapped to 11p15.5. The cDNA encodes a 412-amino acid protein with 20 and 44 amino acids in a pre- and prosegment, respectively. Cathepsin D is one of the lysosomal proteinases. It is ubiquitously expressed and is involved in proteolytic degradation, cell invasion, and apoptosis. Mutations in this gene are involved in the pathogenesis of several diseases, including breast cancer and possibly Alzheimer disease and it has been considered as a breast cancer tumor marker. Key=gene_name, value=CTSD Key=sample_type, value=cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) Key=kit_components, value=10 ng/tube Key=intra_inter_assay_cv, value=
Intra-Assay Precision
Inter-Assay Precision
Sample
1
2
3
1
2
3
n
16
16
16
24
24
24
Mean (pg/ml)
191
1701
5274
179
1699
5098
Standard deviation
13.56
100.36
358.63
13.43
108.74
433.33
CV (%)
7.1%
5.9%
6.8%
7.5%
6.4%
8.5%
Key=sample_data, value=
Concentration (pg/ml)
0
156
312
625
1250
2500
5000
10000
O.D.
0.052
0.123
0.255
0.462
0.775
1.343
1.769
2.094
Key=competitor_equivalent_skus, value= Key=immunogen, value=Expression system for standard: NS0; Immunogen sequence: L21-L412 Key=storage, value=Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.) Key=reproducibility, value=
Lots
Lot 1 (pg/ml)
Lot 2 (pg/ml)
Lot 3 (pg/ml)
Lot 4 (pg/ml)
Mean (pg/ml)
Standard Deviation
CV (%)
Sample 1
191
201
226
217
209
11.48
5.5%
Sample 2
1701
1814
1695
1596
1702
76.57
4.5%
Sample 3
5274
5267
4806
4977
5081
157.51
3.1%
*number of samples for each test n=16. Key=custom_attribute_1, value=EK0672 Key=custom_attribute_2, value=1 Key=custom_attribute_3, value=3ml Key=options Key=media_gallery Key=extension_attributes Key=tier_price Key=tier_price_changed Key=category_ids Key=is_salable Key=website_ids Key=request_path Key=rating_summary Key=_cache_instance_store_filter Key=media_gallery_images
Human Cathepsin D PicoKine® Quick ELISA Kit
Cathepsin D ELISA kit, 90-minute one-step protocol
Human Cathepsin D PicoKine® Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Human CTSD in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
*Question: How many samples can I assay/run in this kit?
Description
Human Cathepsin D PicoKine® Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Human CTSD in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)
Cite This Product
Human Cathepsin D PicoKine® Quick ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # FEK0672)
Cathepsin D is a protein that in humans is encoded by the CTSD gene. This proteinase is a member of the peptidase C1 family, having a specificity similar to but narrower than that of pepsin A. It is mapped to 11p15.5. The cDNA encodes a 412-amino acid protein with 20 and 44 amino acids in a pre- and prosegment, respectively. Cathepsin D is one of the lysosomal proteinases. It is ubiquitously expressed and is involved in proteolytic degradation, cell invasion, and apoptosis. Mutations in this gene are involved in the pathogenesis of several diseases, including breast cancer and possibly Alzheimer disease and it has been considered as a breast cancer tumor marker.
Kit Components
Catalog number
Description
Quantity
Volume
FEK0672-CAP
Anti-tag Pre-coated 96-well Strip Microplate
1
12 strips of 8 wells
EK0672-ST
Human Cathepsin D Standard
2
10 ng/tube
FEK0672-A
Human CTSD Antibody A
1
3ml
FEK0672-B
Human CTSD Antibody B
1
3ml
AR1106-1
Sample Diluent
1
15ml
AR1106-7
TBS-T Wash Buffer (25x)
1
12ml
AR1104
Color Developing Reagent (TMB)
1
10ml
AR1105
Stop Solution
1
10ml
PLA-SEA
Adhesive Plate Sealers
2
Piece
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
Microplate Reader capable of reading absorbance at 450nm.
Automated plate washer (optional)
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.
Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.
Human Cathepsin D PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human Cathepsin D PicoKine® Quick ELISA Kit within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
Intra-Assay Precision
Inter-Assay Precision
Sample
1
2
3
1
2
3
n
16
16
16
24
24
24
Mean (pg/ml)
191
1701
5274
179
1699
5098
Standard deviation
13.56
100.36
358.63
13.43
108.74
433.33
CV (%)
7.1%
5.9%
6.8%
7.5%
6.4%
8.5%
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of Cathepsin D in ELISA kits from four different production batches/lots.
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on CTSD, check out the CTSD Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for CTSD: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].