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Product Info Summary
| SKU: | PB9854 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Cathepsin D/CTSD Antibody Picoband®
View all Cathepsin D Antibodies
SKU/Catalog Number
PB9854
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Cathepsin D/CTSD Antibody Picoband® catalog # PB9854. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Cathepsin D/CTSD Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9854)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human Cathepsin D recombinant protein (Position: M201-L412). Human Cathepsin D shares 84.4% and 84% amino acid (aa) sequence identity with mouse and rat Cathepsin D, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
PB9854 is reactive to CTSD in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
28, 45 kDa
Calculated molecular weight
44552 MW
Background of Cathepsin D
Cathepsin D is a protein that in humans is encoded by the CTSD gene. This proteinase is a member of the peptidase C1 family, having a specificity similar to but narrower than that of pepsin A. It is mapped to 11p15.5. The cDNA encodes a 412-amino acid protein with 20 and 44 amino acids in a pre- and prosegment, respectively. Cathepsin D is one of the lysosomal proteinases. It is ubiquitously expressed and is involved in proteolytic degradation, cell invasion, and apoptosis. Mutations in this gene are involved in the pathogenesis of several diseases, including breast cancer and possibly Alzheimer disease and it has been considered as a breast cancer tumor marker.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9854 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Immunocytochemistry/Immunofluorescence, 2μg/ml, Human
Flow Cytometry (Fixed), 1-3μg/1x106 cells, Human
Positive Control
WB: human HepG2 whole cell, human A431 whole cell, human MCF-7 whole cell, human Jurkat whole cell
IHC: human intestinal cancer tissue
ICC/IF: MCF-7 cell
FCM: SiHa cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of CTSD using anti-CTSD antibody (PB9854).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates,
Lane 2: human A431 whole cell lysates,
Lane 3: human MCF-7 whole cell lysates,
Lane 4: human Jurkat whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CTSD antigen affinity purified polyclonal antibody (Catalog # PB9854) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CTSD at approximately 28, 45 kDa. The expected band size for CTSD is at 45 kDa.
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Figure 2. IHC analysis of Cathepsin D using anti-Cathepsin D antibody (PB9854).
Cathepsin D was detected in paraffin-embedded section of human intestinal cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Cathepsin D Antibody (PB9854) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
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Figure 3. Flow Cytometry analysis of SiHa cells using anti-CTSD antibody (PB9854).
Overlay histogram showing SiHa cells stained with PB9854 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CTSD Antibody (PB9854,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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Figure 4. IF analysis of CTSD using anti-CTSD antibody (PB9854).
CTSD was detected in immunocytochemical section of MCF-7 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti-CTSD Antibody (PB9854) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Protein Target Info & Infographic
Gene/Protein Information For CTSD (Source: Uniprot.org, NCBI)
Gene Name
CTSD
Full Name
Cathepsin D
Weight
44552 MW
Superfamily
peptidase A1 family
Alternative Names
Cathepsin D;3.4.23.5;Cathepsin D light chain;Cathepsin D heavy chain;CTSD;CPSD; CTSD CLN10, CPSD, HEL-S-130P cathepsin D cathepsin D|ceroid-lipofuscinosis, neuronal 10|epididymis secretory sperm binding protein Li 130P|lysosomal aspartyl peptidase|lysosomal aspartyl protease
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CTSD, check out the CTSD Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CTSD: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Cathepsin D/CTSD Antibody Picoband® (PB9854)
Hello CJ!
PB9854 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Inhibition of autophagosome-lysosome fusion by ginsenoside Ro via the ESR2-NCF1-ROS pathway sensitizes esophageal cancer cells to 5-fluorouracil-induced cell death via the CHEK1-mediated DNA damage checkpoint
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Customer Q&As
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5 Customer Q&As for Anti-Cathepsin D/CTSD Antibody Picoband®
Question
We purchased anti-Cathepsin D/CTSD antibody for ICC on melanoma in a previous experiment. I am using human, and We want to use the antibody for Flow Cytometry next. you antibody examining melanoma as well as plasma in our next experiment. Could you please give me some suggestion on which antibody would work the best for Flow Cytometry?
Verified Customer
Verified customer
Asked: 2020-03-03
Answer
I looked at the website and datasheets of our anti-Cathepsin D/CTSD antibody and it seems that PB9854 has been validated on human in both ICC and Flow Cytometry. Thus PB9854 should work for your application. Our Boster satisfaction guarantee will cover this product for Flow Cytometry in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for Flow Cytometry detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2020-03-03
Question
My lab would like using your anti-Cathepsin D/CTSD antibody for ceroid lipofuscinosis studies. Has this antibody been tested with western blotting on hepg2 whole cell lysates? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-12-16
Answer
Thanks for your inquiry. This PB9854 anti-Cathepsin D/CTSD antibody is tested on hepg2 whole cell lysates, a549 whole cell lysates, siha cells. It is guaranteed to work for Flow Cytometry, IHC, ICC, WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-12-16
Question
We have observed staining in human adult mammalian kidney. Are there any suggestions? Is anti-Cathepsin D/CTSD antibody supposed to stain adult mammalian kidney positively?
Verified Customer
Verified customer
Asked: 2019-08-30
Answer
From literature adult mammalian kidney does express CTSD. From Uniprot.org, CTSD is expressed in adult mammalian kidney, kidney, liver, melanoma, plasma, platelet, spleen, among other tissues. Regarding which tissues have CTSD expression, here are a few articles citing expression in various tissues:
Kidney, Pubmed ID: 15489334
Liver, Pubmed ID: 1426530, 8393577, 19159218
Melanoma, Pubmed ID: 12643545, 17081065
Plasma, Pubmed ID: 16335952
Platelet, Pubmed ID: 16263699
Spleen, Pubmed ID: 8467789
Boster Scientific Support
Answered: 2019-08-30
Question
We are currently using anti-Cathepsin D/CTSD antibody PB9854 for human tissue, and we are content with the IHC results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2019-05-31
Answer
The anti-Cathepsin D/CTSD antibody (PB9854) has not been validated for cross reactivity specifically with bovine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-05-31
Question
My boss were well pleased with the WB result of your anti-Cathepsin D/CTSD antibody. However we have observed positive staining in spleen lysosome. melanosome. secreted, using this antibody. Is that expected? Could you tell me where is CTSD supposed to be expressed?
Verified Customer
Verified customer
Asked: 2018-03-28
Answer
From literature, spleen does express CTSD. Generally CTSD expresses in lysosome. melanosome. secreted,. Regarding which tissues have CTSD expression, here are a few articles citing expression in various tissues:
Kidney, Pubmed ID: 15489334
Liver, Pubmed ID: 1426530, 8393577, 19159218
Melanoma, Pubmed ID: 12643545, 17081065
Plasma, Pubmed ID: 16335952
Platelet, Pubmed ID: 16263699
Spleen, Pubmed ID: 8467789
Boster Scientific Support
Answered: 2018-03-28
