Product Info Summary
SKU: | M00004 |
---|---|
Size: | 100ug/vial |
Reactive Species: | Chicken, Cow, Dog, Human, Mouse, Rat |
Host: | Mouse |
Application: | Flow Cytometry, IF, IHC, WB |
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Product info
Product Name
Anti-Catenin, beta (p120) CTNNB1 Monoclonal Antibody
View all beta-Catenin Antibodies
SKU/Catalog Number
M00004
Size
100ug/vial
Form
Liquid
Description
Boster Bio Anti-Catenin, beta (p120) CTNNB1 Monoclonal Antibody (Catalog # M00004). Tested in Flow Cytometry, IF, WB, IHC applications. This antibody reacts with Human, Mouse, Rat, Cow, Dog, Chicken.
Storage & Handling
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Cite This Product
Anti-Catenin, beta (p120) CTNNB1 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00004)
Host
Mouse
Contents
Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Clonality
Monoclonal
Clone Number
Clone: 15B8
Isotype
IgG1, kappa
Immunogen
Recombinant chicken β-catenin
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00004 is reactive to CTNNB1 in Chicken, Cow, Dog, Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 100 µL
Calculated molecular weight
85497 MW
Background of beta-Catenin
Beta-catenin associates with the cytoplasmic portion of E-cadherin, which is necessary for the function of E-cadherin as an adhesion molecule. In normal tissues, beta-catenin is localized to the membrane of epithelial cells, consistent with its role in the cell adhesion complex. In breast ductal neoplasia, beta-catenin is usually localized in cellular membranes. However, in lobular neoplasia, a marked redistribution of beta-catenin throughout the cytoplasm results in a diffuse cytoplasmic pattern. Immuno-staining of beta-catenin and E-cadherin is helps in the accurate identification of ductal and lobular neoplasms, including a distinction between low-grade ductal carcinoma in situ (DCIS) and lobular carcinoma. Additionally, some rectal and gastric adenocarcinomas demonstrate diffuse cytoplasmic beta-catenin staining and a lack of membranous staining, mimicking the staining pattern observed with lobular breast carcinomas.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00004 is guaranteed for Flow Cytometry, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.
Validation Images & Assay Conditions
Click image to see more details
Formalin-fixed, paraffin-embedded human tonsil stained with Anti-Beta-Catenin (p120) Monoclonal Antibody (15B8).
Protein Target Info & Infographic
Gene/Protein Information For CTNNB1 (Source: Uniprot.org, NCBI)
Gene Name
CTNNB1
Full Name
Catenin beta-1
Weight
85497 MW
Superfamily
beta-catenin family
Alternative Names
Catenin beta-1;Beta-catenin;CTNNB1;CTNNB;OK/SW-cl.35, PRO2286; CTNNB1 CTNNB, EVR7, MRD19, NEDSDV, armadillo catenin beta 1 catenin beta-1|catenin (cadherin-associated protein), beta 1, 88kDa
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CTNNB1, check out the CTNNB1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CTNNB1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Catenin, beta (p120) CTNNB1 Monoclonal Antibody (M00004)
Hello CJ!
M00004 has been cited in 13 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Xu C,Liu F,Xiang G,Cao L,Wang S,Liu J,Meng Q,Xu D,Lv S,Jiao J,Niu Y.β-Catenin nuclear localization positively feeds back on EGF/EGFR-attenuated AJAP1 expression in breast cancer.J Exp Clin Cancer Res.2019 Jun 6;38(1):238.doi:10.1186/s13046-019-1252-6.PMID:31171012;PMCID:PMC6554977.
Species: Human,Mouse
M00004 usage in article: APP:IHC, SAMPLE:BREAST TISSUE, DILUTION:1:200
Dark Aberrant Crypt Foci with activated Wnt pathway are related to tumorigenesis in the colon of AOM-treated rat
Anti-cancer drug 3, 3%u2032-diindolylmethane activates Wnt4 signaling to enhance gastric cancer cell stemness and tumorigenesis
Oestrogen and parathyroid hormone alleviate lumbar intervertebral disc degeneration in ovariectomized rats and enhance Wnt/?-catenin pathway activity
Targeted p53 activation by saRNA suppresses human bladder cancer cells growth and metastasis
Wnt/?-catenin pathway is required for epithelial to mesenchymal transition in CXCL12 over expressed breast cancer cells
Curcumin alleviates glucocorticoid-induced osteoporosis through the regulation of the Wnt signaling pathway
Prolonged overexpression of Wnt10b induces epidermal keratinocyte transformation through activating EGF pathway
Celastrol Ameliorates Ulcerative Colitis-Related Colorectal Cancer in Mice via Suppressing Inflammatory Responses and Epithelial-Mesenchymal Transition
Combination of lithium chloride and pEGFP-N1-BmK CT effectively decreases proliferation and migration of C6 glioma cells
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