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Product Info Summary
| SKU: | PA1212 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IF, IHC, IHC-F, ICC, WB |
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Product info
Product Name
Anti-beta Catenin/CTNNB1 Antibody Picoband®
View all beta-Catenin Antibodies
SKU/Catalog Number
PA1212
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-beta Catenin/CTNNB1 Antibody catalog # PA1212. Tested in IF, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-beta Catenin/CTNNB1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1212)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human beta Catenin, identical to the related rat and mouse sequences.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1212 is reactive to CTNNB1 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
95 kDa
Calculated molecular weight
85497 MW
Background of beta-Catenin
Catenins are proteins found in complexes with cadherin cell adhesion molecules of animal cells. The first two catenins that were identified became known as alpha-catenin and beta-catenin. Alpha-catenin can bind to beta-catenin and can also bind actin. Beta-catenin binds the cytoplasmic domain of some cadherins. Beta-catenin is an adherens junction protein. It plays an important role in various aspects of liver biology including liver development (both embryonic and postnatal), liver regeneration following partial hepatectomy. HGF-induced hepatpomegaly, liver zonation, and pathogenesis of liver cancer.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1212 is guaranteed for IF, IHC, IHC-F, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat
Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Mouse, Rat, Human
Immunocytochemistry , 0.5-1μg/ml, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5μg/ml, Human
Positive Control
WB: MM453 Cell, MCF-7 Cell, HELA Cell
IHC: Human Mammary Cancer Tissue
IHC-F: Rat Intestine Tissue
ICC/IF: A431 cell
Validation Images & Assay Conditions
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Anti-beta Catenin antibody, PA1212, Western blotting
Lane 1: MM453 Cell Lysate
Lane 2: MCF-7 Cell Lysate
Lane 3: HELA Cell Lysate
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Anti-beta Catenin antibody, PA1212, IHC(P)
IHC(P): Human Mammary Cancer Tissue
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Anti-beta Catenin antibody, PA1212, IHC(F)
IHC(F): Rat Intestine Tissue
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Figure 4. IF analysis of Beta Catenin using anti-Beta Catenin antibody (PA1212).
Beta Catenin was detected in immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL rabbit anti-Beta Catenin Antibody (PA1212) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Protein Target Info & Infographic
Gene/Protein Information For CTNNB1 (Source: Uniprot.org, NCBI)
Gene Name
CTNNB1
Full Name
Catenin beta-1
Weight
85497 MW
Superfamily
beta-catenin family
Alternative Names
Catenin beta-1;Beta-catenin;CTNNB1;CTNNB;OK/SW-cl.35, PRO2286; CTNNB1 CTNNB, EVR7, MRD19, NEDSDV, armadillo catenin beta 1 catenin beta-1|catenin (cadherin-associated protein), beta 1, 88kDa
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CTNNB1, check out the CTNNB1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CTNNB1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-beta Catenin/CTNNB1 Antibody Picoband® (PA1212)
Hello CJ!
PA1212 has been cited in 23 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
LINC00184 involved in the regulatory network of ANGPT2 via ceRNA mediated miR-145 inhibition in gastric cancer
Histone deacetylase HDAC4 promotes the proliferation and invasion of glioma cells
Novel chemiluminescent Western blot blocking and antibody incubation solution for enhanced antibody‐antigen interaction and increased specificity
Piao HY,Guo S,Wang Y,Zhang J.Exosome-transmitted lncRNA PCGEM1 promotes invasive and metastasis in gastric cancer by maintaining the stability of SNAI1.Clin Transl Oncol.2020 Jun 9.doi:10.1007/s12094-020-02412-9.Epub ahead of print.PMID:32519176.
Species: Human
PA1212 usage in article: APP:WB, SAMPLE:AGS CELL AND MKN45 CELL, DILUTION:1:200
Dark Aberrant Crypt Foci with activated Wnt pathway are related to tumorigenesis in the colon of AOM-treated rat
Anti-cancer drug 3, 3%u2032-diindolylmethane activates Wnt4 signaling to enhance gastric cancer cell stemness and tumorigenesis
Effects of atorvastatin on porcine aqueous humour outflow and trabecular meshwork cells
MgCl2 and ZnCl2 promote human umbilical vein endothelial cell migration and invasion and stimulate epithelial-mesenchymal transition via the Wnt/?-catenin pathway
Oestrogen and parathyroid hormone alleviate lumbar intervertebral disc degeneration in ovariectomized rats and enhance Wnt/?-catenin pathway activity
Targeted p53 activation by saRNA suppresses human bladder cancer cells growth and metastasis
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