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Product Info Summary
| SKU: | PA1735 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-Caspase-8(p18)/CASP8 Antibody Picoband®
SKU/Catalog Number
PA1735
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Caspase-8 (p18)/CASP8 Antibody catalog # PA1735. Tested in IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Caspase-8(p18)/CASP8 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1735)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human Caspase-8(p18).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1735 is reactive to CASP8 in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
18 kDa
Calculated molecular weight
55391 MW
Background of Caspase-8
Caspase 8 is a caspase protein. It most likely acts upon caspase 3. This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. The human CASP8 gene, whose product is also known as caspase 8 and FLICE, encodes an interleukin-1beta converting enzyme (ICE)-related cysteine protease that is activated by the engagement of several different death receptors. Caspase 8 is immediately recruited to the Fas receptor once it oligomerizes, and its protease activity is crucial for the apoptotic response generated by the resulting death-inducing signaling complex (DISC). This gene contains at least 11 exons spanning approximately 30kb on human chromosome band 2q33-34. This region of human chromosome 2 was previously reported as the location of the CASP10 gene, whose product is closely related to caspase 8. Caspase-8 deficiency in humans is compatible with normal development and shows that caspase-8 has a postnatal role in immune activation of naive lymphocytes.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1735 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Western blot, 0.1-0.5μg/ml, Human
Positive Control
WB: A549 Whole Cell, HELA Whole Cell
IHC: Human Tonsil Tissue
Validation Images & Assay Conditions
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Anti-Caspase-8(p18) antibody, PA1735, Western blotting
All lanes: Anti Caspase-8(p18) (PA1735) at 0.5ug/ml
Lane 1: A549 Whole Cell Lysate at 40ug
Lane 2: HELA Whole Cell Lysate at 40ug
Predicted bind size: 18KD
Observed bind size: 18KD
Click image to see more details
Anti-Caspase-8(p18) antibody, PA1735,IHC(P)
IHC(P): Human Tonsil Tissue
Protein Target Info & Infographic
Gene/Protein Information For CASP8 (Source: Uniprot.org, NCBI)
Gene Name
CASP8
Full Name
Caspase-8
Weight
55391 MW
Superfamily
peptidase C14A family
Alternative Names
Caspase-8;CASP-8;3.4.22.61;Apoptotic cysteine protease;Apoptotic protease Mch-5;CAP4;FADD-homologous ICE/ced-3-like protease;FADD-like ICE;FLICE;ICE-like apoptotic protease 5;MORT1-associated ced-3 homolog;MACH;Caspase-8 subunit p18;Caspase-8 subunit p10;CASP8;MCH5; CASP8 ALPS2B, CAP4, Casp-8, FLICE, MACH, MCH5 caspase 8 caspase-8|FADD-homologous ICE/CED-3-like protease|FADD-like ICE|ICE-like apoptotic protease 5|MACH-alpha-1/2/3 protein|MACH-beta-1/2/3/4 protein|MORT1-associated ced-3 homolog|apoptotic cysteine protease|apoptotic protease Mch-5|caspase 8, apoptosis-related cysteine peptidase|caspase 8, apoptosis-related cysteine protease
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CASP8, check out the CASP8 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CASP8: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Caspase-8(p18)/CASP8 Antibody Picoband® (PA1735)
Hello CJ!
PA1735 has been cited in 16 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Inhibition of proliferation of prostate cancer cell line, PC-3, in vitro and in vivo using (%u2212)-gossypol
Protective effect of RIP and c-FLIP in preventing liver cancer cell apoptosis induced by TRAIL
Inhibitory activity of apogossypol in human prostate cancerin vitroandin vivo
Intravenous Administration Is an Effective and Safe Route for Cancer Gene Therapy Using the Bifidobacterium-Mediated Recombinant HSV-1 Thymidine Kinase and Ganciclovir
Inhibition of proliferation of prostate cancer cell line, PC-3, in vitro and in vivo using (?)-gossypol
Croton Tiglium Extract Induces Apoptosis via Bax/Bcl-2 Pathways in Human Lung Cancer A549 Cells
2-DG-Regulated RIP and c-FLIP Effect on Liver Cancer Cell Apoptosis Induced by TRAIL
Liu N, Chen T, Wang X, Yang D, Xue B, Zhu H. Febs Lett. 2015 Apr 2;589(8):897-903. Doi: 10.1016/J.Febslet.2015.02.026. Epub 2015 Mar 3. Msi1 Confers Resistance To Trail By Activating Erk In Liver Cancer Cells.
Lin Hd, He Cq, Luo Ql, Zhang Jl, Zeng Dx. Rheumatol Int. 2012 Mar;32(3):759-66. Doi: 10.1007/S00296-010-1629-5. Epub 2010 Dec 28. The Effect Of Low-Level Laser To Apoptosis Of Chondrocyte And Caspases Expression, Including Caspase-8 And Caspase-3 ...
Guo H, Luo Q, Zhang J, Lin H, Xia L, He C. Joint Bone Spine. 2011 Dec;78(6):604-10. Doi: 10.1016/J.Jbspin.2011.01.009. Epub 2011 Mar 11. Comparing Different Physical Factors On Serum Tnf-?? Levels, Chondrocyte Apoptosis, Caspase-3 And Caspase-8 Ex...
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Customer Reviews
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Customer Q&As
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17 Customer Q&As for Anti-Caspase-8(p18)/CASP8 Antibody Picoband®
Question
Is this PA1735 anti-Caspase-8(p18)/CASP8 antibody reactive to the isotypes of CASP8?
Verified Customer
Verified customer
Asked: 2019-12-17
Answer
The immunogen of PA1735 anti-Caspase-8(p18)/CASP8 antibody is A synthetic peptide corresponding to a sequence at the N-terminus of human Caspase-8 (p18) (257-277aa NNHNFAKAREKVPKLHSIRDR). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-12-17
Question
We want using your anti-Caspase-8(p18)/CASP8 antibody for activation of cysteine-type endopeptidase activity involved in apoptotic process studies. Has this antibody been tested with western blotting on hela whole cell lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-10-29
Answer
We appreciate your inquiry. This PA1735 anti-Caspase-8(p18)/CASP8 antibody is validated on a549 whole cell lysate, hela whole cell lysate, human tonsil tissue. It is guaranteed to work for IHC, WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-10-29
Question
Is a blocking peptide available for product anti-Caspase-8(p18)/CASP8 antibody (PA1735)?
Verified Customer
Verified customer
Asked: 2019-08-15
Answer
We do provide the blocking peptide for product anti-Caspase-8(p18)/CASP8 antibody (PA1735). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-08-15
Question
Would anti-Caspase-8(p18)/CASP8 antibody PA1735 work for IHC with t-cell?
Verified Customer
Verified customer
Asked: 2019-07-17
Answer
According to the expression profile of t-cell, CASP8 is highly expressed in t-cell. So, it is likely that anti-Caspase-8(p18)/CASP8 antibody PA1735 will work for IHC with t-cell.
Boster Scientific Support
Answered: 2019-07-17
Question
Will PA1735 anti-Caspase-8(p18)/CASP8 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-07-01
Answer
You can see on the product datasheet, PA1735 anti-Caspase-8(p18)/CASP8 antibody as been tested on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-07-01
Question
I got a high background from the bands on my western blot gel. Here are the parameters that I used: - Protein loading 70 µg - SDS gel 10% - Electrophoresis 60V 60 min & 120V 95 min; - Blocking 5% milk RT 30 min - Primary antibody 1:2000 dilution & 4°C overnight incubation - Secondary antibody α-Rabbit –HRP RT incubation for 1 hour Please advise how we can lower the background.
Verified Customer
Verified customer
Asked: 2019-05-31
Answer
Try to repeat your western blot with the following changes: - Protein loading 50 µg - Blocking 5% non-fat dry milk & incubate at 4°C for 2 hours with slow shaking - Primary antibody 1:4000 dilution Then the background of your gel should be reduced.
Boster Scientific Support
Answered: 2019-05-31
Question
We are currently using anti-Caspase-8(p18)/CASP8 antibody PA1735 for human tissue, and we are well pleased with the IHC results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on dog tissues as well?
Verified Customer
Verified customer
Asked: 2019-05-24
Answer
The anti-Caspase-8(p18)/CASP8 antibody (PA1735) has not been validated for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-05-24
Question
We are interested in to test anti-Caspase-8(p18)/CASP8 antibody PA1735 on human t-cell for research purposes, then I may be interested in using anti-Caspase-8(p18)/CASP8 antibody PA1735 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
L. Carter
Verified customer
Asked: 2019-03-14
Answer
The products we sell, including anti-Caspase-8(p18)/CASP8 antibody PA1735, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-03-14
Question
I see that the anti-Caspase-8(p18)/CASP8 antibody PA1735 works with IHC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2018-12-27
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2018-12-27
Question
My question regarding product PA1735, anti-Caspase-8(p18)/CASP8 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
R. Lewis
Verified customer
Asked: 2018-11-21
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA1735 anti-Caspase-8(p18)/CASP8 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-11-21
Question
We ordered your anti-Caspase-8(p18)/CASP8 antibody for IHC on leukocyte a few months ago. I am using human, and We are going to use the antibody for WB next. We need examining leukocyte as well as small intestine peyer's patch in our next experiment. Could give a recommendation on which antibody would work the best for WB?
Verified Customer
Verified customer
Asked: 2018-07-11
Answer
I viewed the website and datasheets of our anti-Caspase-8(p18)/CASP8 antibody and I see that PA1735 has been validated on human in both IHC and WB. Thus PA1735 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2018-07-11
Question
Our team were content with the WB result of your anti-Caspase-8(p18)/CASP8 antibody. However we have been able to see positive staining in b-cell thymus cytoplasm. using this antibody. Is that expected? Could you tell me where is CASP8 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2018-05-30
Answer
From what I have seen in literature, b-cell thymus does express CASP8. Generally CASP8 expresses in cytoplasm. Regarding which tissues have CASP8 expression, here are a few articles citing expression in various tissues:
B-cell, and Thymus, Pubmed ID: 8681376
Leukocyte, Pubmed ID: 12010809, 15489334
T-cell, Pubmed ID: 8755496
Boster Scientific Support
Answered: 2018-05-30
Question
Please see the WB image, lot number and protocol we used for t-cell using anti-Caspase-8(p18)/CASP8 antibody PA1735. Please let me know if you require anything else.
T. Huang
Verified customer
Asked: 2018-03-20
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-03-20
Question
I was wanting to use your anti-Caspase-8(p18)/CASP8 antibody for IHC for human t-cell on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human t-cell identification?
Verified Customer
Verified customer
Asked: 2018-01-11
Answer
You can see on the product datasheet, PA1735 anti-Caspase-8(p18)/CASP8 antibody has been validated for IHC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human t-cell in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-01-11
Question
Is there a BSA free version of anti-Caspase-8(p18)/CASP8 antibody PA1735 available?
A. Wu
Verified customer
Asked: 2017-11-17
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Caspase-8(p18)/CASP8 antibody PA1735 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2017-11-17
Question
We have observed staining in human leukocyte. What should we do? Is anti-Caspase-8(p18)/CASP8 antibody supposed to stain leukocyte positively?
D. Jackson
Verified customer
Asked: 2014-12-25
Answer
Based on literature leukocyte does express CASP8. Based on Uniprot.org, CASP8 is expressed in small intestine peyer's patch, b-cell thymus, t-cell, leukocyte, among other tissues. Regarding which tissues have CASP8 expression, here are a few articles citing expression in various tissues:
B-cell, and Thymus, Pubmed ID: 8681376
Leukocyte, Pubmed ID: 12010809, 15489334
T-cell, Pubmed ID: 8755496
Boster Scientific Support
Answered: 2014-12-25
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for t-cell using anti-Caspase-8(p18)/CASP8 antibody PA1735. Let me know if you need anything else.
J. Krishna
Verified customer
Asked: 2013-12-11
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2013-12-11
