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Product Info Summary
| SKU: | PA1308 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Caspase-8 (P18)/CASP8 Antibody Picoband®
SKU/Catalog Number
PA1308
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Caspase-8 (P18)/CASP8 Antibody catalog # PA1308. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Caspase-8 (P18)/CASP8 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1308)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human Caspase-8(P18), different from the related mouse sequence by one amino acid.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1308 is reactive to CASP8 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
55 kDa
Calculated molecular weight
55391 MW
Background of Caspase-8
Caspase 8 is a caspase protein. It most likely acts upon caspase 3. This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. The human CASP8 gene, whose product is also known as caspase 8 and FLICE, encodes an interleukin-1beta converting enzyme (ICE)-related cysteine protease that is activated by the engagement of several different death receptors. Caspase 8 is immediately recruited to the Fas receptor once it oligomerizes, and its protease activity is crucial for the apoptotic response generated by the resulting death-inducing signaling complex (DISC). This gene contains at least 11 exons spanning approximately 30kb on human chromosome band 2q33-34. This region of human chromosome 2 was previously reported as the location of the CASP10 gene, whose product is closely related to caspase 8. Caspase-8 deficiency in humans is compatible with normal development and shows that caspase-8 has a postnatal role in immune activation of naive lymphocytes.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1308 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse
Positive Control
WB: Rat Thymus Tissue, Rat Liver Tissue, MCF-7 Whole Cell, HELA Whole Cell, SMMC Whole Cell
Validation Images & Assay Conditions
Click image to see more details
Anti-Caspase-8(P18) antibody, PA1308, Western blotting
All lanes: Anti Caspase-8(P18) (PA1308) at 0.5ug/ml
Lane 1: Rat Thymus Tissue Lysate at 50ug
Lane 2: Rat Liver Tissue Lysate at 50ug
Lane 3: MCF-7 Whole Cell Lysate at 40ug
Lane 4: HELA Whole Cell Lysate at 40ug
Lane 5: SMMC Whole Cell Lysate at 40ug
Predicted bind size: 55KD
Observed bind size: 55KD
Protein Target Info & Infographic
Gene/Protein Information For CASP8 (Source: Uniprot.org, NCBI)
Gene Name
CASP8
Full Name
Caspase-8
Weight
55391 MW
Superfamily
peptidase C14A family
Alternative Names
Caspase-8;CASP-8;3.4.22.61;Apoptotic cysteine protease;Apoptotic protease Mch-5;CAP4;FADD-homologous ICE/ced-3-like protease;FADD-like ICE;FLICE;ICE-like apoptotic protease 5;MORT1-associated ced-3 homolog;MACH;Caspase-8 subunit p18;Caspase-8 subunit p10;CASP8;MCH5; CASP8 ALPS2B, CAP4, Casp-8, FLICE, MACH, MCH5 caspase 8 caspase-8|FADD-homologous ICE/CED-3-like protease|FADD-like ICE|ICE-like apoptotic protease 5|MACH-alpha-1/2/3 protein|MACH-beta-1/2/3/4 protein|MORT1-associated ced-3 homolog|apoptotic cysteine protease|apoptotic protease Mch-5|caspase 8, apoptosis-related cysteine peptidase|caspase 8, apoptosis-related cysteine protease
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CASP8, check out the CASP8 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CASP8: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Caspase-8 (P18)/CASP8 Antibody Picoband® (PA1308)
Hello CJ!
PA1308 has been cited in 17 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Zhou L,Yu W,Jayabalan DS,Niesvizky R,Jaffrey SR,Huang X,Xu G.Caspase-8 Inhibition Prevents the Cleavage and Degradation of E3 Ligase Substrate Receptor Cereblon and Potentiates Its Biological Function.Front Cell Dev Biol.2020 Dec 17;8:605989.doi:10.3389/f
Species: Human
PA1308 usage in article: APP:WB, SAMPLE:HEK293 CELL AND HELA CELL, DILUTION:NA
Inhibition of proliferation of prostate cancer cell line, PC-3, in vitro and in vivo using (%u2212)-gossypol
Protective effect of RIP and c-FLIP in preventing liver cancer cell apoptosis induced by TRAIL
Inhibitory activity of apogossypol in human prostate cancerin vitroandin vivo
Intravenous Administration Is an Effective and Safe Route for Cancer Gene Therapy Using the Bifidobacterium-Mediated Recombinant HSV-1 Thymidine Kinase and Ganciclovir
Inhibition of proliferation of prostate cancer cell line, PC-3, in vitro and in vivo using (?)-gossypol
Croton Tiglium Extract Induces Apoptosis via Bax/Bcl-2 Pathways in Human Lung Cancer A549 Cells
2-DG-Regulated RIP and c-FLIP Effect on Liver Cancer Cell Apoptosis Induced by TRAIL
Liu N, Chen T, Wang X, Yang D, Xue B, Zhu H. Febs Lett. 2015 Apr 2;589(8):897-903. Doi: 10.1016/J.Febslet.2015.02.026. Epub 2015 Mar 3. Msi1 Confers Resistance To Trail By Activating Erk In Liver Cancer Cells.
Lin Hd, He Cq, Luo Ql, Zhang Jl, Zeng Dx. Rheumatol Int. 2012 Mar;32(3):759-66. Doi: 10.1007/S00296-010-1629-5. Epub 2010 Dec 28. The Effect Of Low-Level Laser To Apoptosis Of Chondrocyte And Caspases Expression, Including Caspase-8 And Caspase-3 ...
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Customer Reviews
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Customer Q&As
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16 Customer Q&As for Anti-Caspase-8 (P18)/CASP8 Antibody Picoband®
Question
Is this PA1308 anti-Caspase-8 (P18)/CASP8 antibody reactive to the isotypes of CASP8?
Verified Customer
Verified customer
Asked: 2020-02-10
Answer
The immunogen of PA1308 anti-Caspase-8 (P18)/CASP8 antibody is A synthetic peptide corresponding to a sequence in the middle region of human Caspase-8(P18)(240-259aa DKVYQMKSKPRGYCLIINNH), different from the related mouse sequence by one amino acid. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-02-10
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for t-cell using anti-Caspase-8 (P18)/CASP8 antibody PA1308. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-01-24
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-01-24
Question
We have observed staining in human leukocyte. Do you have any suggestions? Is anti-Caspase-8 (P18)/CASP8 antibody supposed to stain leukocyte positively?
Verified Customer
Verified customer
Asked: 2019-12-30
Answer
According to literature leukocyte does express CASP8. According to Uniprot.org, CASP8 is expressed in small intestine peyer's patch, b-cell thymus, t-cell, leukocyte, among other tissues. Regarding which tissues have CASP8 expression, here are a few articles citing expression in various tissues:
B-cell, and Thymus, Pubmed ID: 8681376
Leukocyte, Pubmed ID: 12010809, 15489334
T-cell, Pubmed ID: 8755496
Boster Scientific Support
Answered: 2019-12-30
Question
I have a question about product PA1308, anti-Caspase-8 (P18)/CASP8 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-10-14
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA1308 anti-Caspase-8 (P18)/CASP8 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-10-14
Question
I was wanting to use your anti-Caspase-8 (P18)/CASP8 antibody for WB for human t-cell on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human t-cell identification?
Verified Customer
Verified customer
Asked: 2019-08-30
Answer
You can see on the product datasheet, PA1308 anti-Caspase-8 (P18)/CASP8 antibody has been tested for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human t-cell in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-08-30
Question
Is a blocking peptide available for product anti-Caspase-8 (P18)/CASP8 antibody (PA1308)?
Verified Customer
Verified customer
Asked: 2019-08-23
Answer
We do provide the blocking peptide for product anti-Caspase-8 (P18)/CASP8 antibody (PA1308). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-08-23
Question
I see that the anti-Caspase-8 (P18)/CASP8 antibody PA1308 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-07-25
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-07-25
Question
Does PA1308 anti-Caspase-8 (P18)/CASP8 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Z. Jha
Verified customer
Asked: 2018-12-18
Answer
As indicated on the product datasheet, PA1308 anti-Caspase-8 (P18)/CASP8 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-12-18
Question
Here is the WB image, lot number and protocol we used for t-cell using anti-Caspase-8 (P18)/CASP8 antibody PA1308. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2018-03-12
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-03-12
Question
Does anti-Caspase-8 (P18)/CASP8 antibody PA1308 work for WB with t-cell?
Verified Customer
Verified customer
Asked: 2017-10-16
Answer
According to the expression profile of t-cell, CASP8 is highly expressed in t-cell. So, it is likely that anti-Caspase-8 (P18)/CASP8 antibody PA1308 will work for WB with t-cell.
Boster Scientific Support
Answered: 2017-10-16
Question
Our lab were happy with the WB result of your anti-Caspase-8 (P18)/CASP8 antibody. However we have observed positive staining in t-cell cytoplasm. using this antibody. Is that expected? Could you tell me where is CASP8 supposed to be expressed?
J. Banerjee
Verified customer
Asked: 2017-06-27
Answer
From what I have seen in literature, t-cell does express CASP8. Generally CASP8 expresses in cytoplasm. Regarding which tissues have CASP8 expression, here are a few articles citing expression in various tissues:
B-cell, and Thymus, Pubmed ID: 8681376
Leukocyte, Pubmed ID: 12010809, 15489334
T-cell, Pubmed ID: 8755496
Boster Scientific Support
Answered: 2017-06-27
Question
I am interested in using your anti-Caspase-8 (P18)/CASP8 antibody for apoptotic cleavage of cellular proteins studies. Has this antibody been tested with western blotting on liver tissue? We would like to see some validation images before ordering.
R. Johnson
Verified customer
Asked: 2015-11-09
Answer
Thanks for your inquiry. This PA1308 anti-Caspase-8 (P18)/CASP8 antibody is tested on rat thymus tissue, tissue lysate, liver tissue, hela whole cell lysate, smmc whole cell lysate. It is guaranteed to work for WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2015-11-09
Question
Will anti-Caspase-8 (P18)/CASP8 antibody PA1308 work on feline WB with b-cell thymus?
J. Jones
Verified customer
Asked: 2015-05-25
Answer
Our lab technicians have not tested anti-Caspase-8 (P18)/CASP8 antibody PA1308 on feline. You can run a BLAST between feline and the immunogen sequence of anti-Caspase-8 (P18)/CASP8 antibody PA1308 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated feline samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in feline b-cell thymus in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2015-05-25
Question
Is there a BSA free version of anti-Caspase-8 (P18)/CASP8 antibody PA1308 available?
P. Miller
Verified customer
Asked: 2014-06-02
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-Caspase-8 (P18)/CASP8 antibody PA1308 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2014-06-02
Question
We need to test anti-Caspase-8 (P18)/CASP8 antibody PA1308 on human t-cell for research purposes, then I may be interested in using anti-Caspase-8 (P18)/CASP8 antibody PA1308 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
R. Krishna
Verified customer
Asked: 2013-05-03
Answer
The products we sell, including anti-Caspase-8 (P18)/CASP8 antibody PA1308, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2013-05-03
Question
We are currently using anti-Caspase-8 (P18)/CASP8 antibody PA1308 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on canine tissues as well?
V. Williams
Verified customer
Asked: 2013-04-03
Answer
The anti-Caspase-8 (P18)/CASP8 antibody (PA1308) has not been validated for cross reactivity specifically with canine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2013-04-03
