Anti-Caspase-3 CASP3 Antibody

Product Name

Anti-Caspase-3 CASP3 Antibody

View all Caspase-3 Antibodies

SKU/Catalog Number

A00334

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-Caspase-3 CASP3 Antibody (Catalog # A00334). Tested in ELISA, IHC-P applications. This antibody reacts with Human.

Storage & Handling

Caspase-3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-Caspase-3 CASP3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00334)

Host

Rabbit

Contents

Caspase-3 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Caspase-3 antibody was raised against a 17 amino acid synthetic peptide near the center of human Caspase-3. The immunogen is located within amino acids 70 - 120 of Caspase-3.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

At least two isoforms of Caspase-3 are known to exist.

Reactive Species

A00334 is reactive to CASP3 in Human

Reconstitution

Observed Molecular Weight

68 kDa

Calculated molecular weight

31608 MW

Background of Caspase-3

Caspases are a family of cysteine proteases that can be divided into the apoptotic and inflammatory caspase subfamilies. Unlike the apoptotic caspases, members of the inflammatory subfamily are generally not involved in cell death but are associated with the immune response to microbial pathogens. The apoptotic subfamily can be further divided into initiator caspases, which are activated in response to death signals, and executioner caspases, which are activated by the initiator caspases and are responsible for cleavage of cellular substrates that ultimately lead to cell death. Caspase-3 is synthesized as an inactive proenzyme that undergoes proteolytic cleavage by caspases 8, 9 and 10 to produce 2 subunits, termed p20 and p11. These subunits dimerize to form the active enzyme. Caspase-3 proteolytically cleaves and activates other proteins such as caspases 6, 7 and 9.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A00334 is guaranteed for ELISA, IHC-P Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Caspase-3 antibody can be used for detection of Caspase-3 by immunohistochemistry at 5 μg/mL.
Antibody validated: Immunohistochemistry in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

Gene/Protein Information For CASP3 (Source: Uniprot.org, NCBI)

Alternative Names

CPP32, SCA-1, CPP32B, CPP32, Caspase-3, Apopain, CASP-3 CASP3 CPP32, CPP32B, SCA-1 caspase 3 caspase-3|CASP-3|CPP-32|PARP cleavage protease|SREBP cleavage activity 1|apopain|caspase 3, apoptosis-related cysteine peptidase|caspase 3, apoptosis-related cysteine protease|cysteine protease CPP32|procaspase3|protein Yama

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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